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Method for separating anti-tumor polysaccharide component from black fungus by applying radial chromatography

The technology of black fungus polysaccharide and black fungus is applied in the directions of anti-tumor drugs, drug combinations, etc., can solve the problems of insignificant anti-colon cancer activity and the like, and achieve the effects of convenient industrial production, high yield and good therapeutic effect.

Inactive Publication Date: 2012-08-29
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Its defects are mainly: the anti-colon cancer activity is not obvious, only a certain activity of scavenging free radicals

Method used

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  • Method for separating anti-tumor polysaccharide component from black fungus by applying radial chromatography
  • Method for separating anti-tumor polysaccharide component from black fungus by applying radial chromatography
  • Method for separating anti-tumor polysaccharide component from black fungus by applying radial chromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1, a kind of method that applies radial chromatography to separate antitumor polysaccharide component from black fungus, carries out following steps successively:

[0049] 1) Put 1,000g of black fungus into 25,000ml of hot water at a temperature of 65-70°C and insulate and extract for 180 minutes to obtain black fungus polysaccharide extract.

[0050] 2) Concentrate the black fungus polysaccharide extract with a rotary evaporator (100Pa, 40°C) to obtain a concentrated solution; the carbohydrate content in the concentrated solution is 2.8 mg / ml; the phenol-sulfuric acid colorimetric method can be used to detect the concentration in the concentrated solution carbohydrate content;

[0051] Add Savag reagent accounting for 50% of the volume of the concentrated solution to the concentrated solution, shake repeatedly, and then centrifuge at 3000 rpm for 15 minutes to remove the protein precipitate, and obtain the supernatant as a polysaccharide aqueous solution fro...

Embodiment 2

[0062] Embodiment 2, a method of applying radial chromatography to separate anti-tumor polysaccharide components from black fungus, the following steps are carried out in sequence:

[0063] 1) Put 500g of black fungus into 10,000ml of hot water at a temperature of 70°C and insulate and extract for 180 minutes to obtain black fungus polysaccharide extract.

[0064] 2) Concentrate the black fungus polysaccharide extract with a rotary evaporator (100Pa, 40°C) to obtain a concentrated solution; the carbohydrate content in the concentrated solution is 3.0mg / ml; use the phenol-sulfuric acid colorimetric method to detect the carbohydrates in the concentrated solution the content of the compound;

[0065] Add Savag reagent accounting for 50% of the volume of the concentrated solution to the concentrated solution, shake repeatedly, and then centrifuge at 3000 rpm for 20 minutes to remove the protein precipitate, and obtain the supernatant as a polysaccharide aqueous solution from which...

Embodiment 3

[0075] Embodiment 3, a kind of method that applies radial chromatography to separate antitumor polysaccharide component from black fungus, carries out the following steps successively:

[0076] 1) Put 1,500g of black fungus into 30,000ml of hot water at 70°C for 200 minutes to obtain black fungus polysaccharide extract.

[0077] 2) Concentrate the black fungus polysaccharide extract with a rotary evaporator (100Pa, 39°C) to obtain a concentrated solution; the carbohydrate content in the concentrated solution is 2.5mg / ml; use the phenol-sulfuric acid colorimetric method to detect the carbohydrates in the concentrated solution the content of the compound;

[0078] Add Savag reagent accounting for 50% of the volume of the concentrated solution to the concentrated solution, shake repeatedly, and then centrifuge at 3000 rpm for 15 minutes to remove the protein precipitate, and obtain the supernatant as a polysaccharide aqueous solution from which the protein has been removed;

[0...

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Abstract

The invention discloses a method for separating an anti-tumor polysaccharide component from a black fungus by applying radial chromatography. The method comprises the following steps of: 1) extracting the black fungus with hot water; 2) adding Savag agent after concentrating the extracting liquid of the black fungus polysaccharide, shaking and centrifuging; 3) adding alcohol with the volume concentration being more than or equal to 95% into a polysaccharide aqueous solution free from protein, performing alcohol precipitation, and cleaning the obtained precipitation; 4) carrying out freeze drying on coarse black fungus polysaccharide with anti-tumor activity; 5) dissolving the freeze-dried coarse black fungus polysaccharide in distilled water, thereby obtaining a sample solution; pumping the sample solution into a radial chromatograph, selecting DEAE-52 cellulose as padding; respectively eluting by using the distilled water and NaCl solution with the concentration of 0.2M; and 6) collecting the eluent corresponding to the NaCl solution with the concentration of 0.2M, and performing dialysis, concentration and drying in turn, thereby obtaining the black fungus polysaccharide with anti-tumor activity.

Description

technical field [0001] The invention belongs to the technical field of functional food development, and relates to a separation and purification method of functional food active ingredient polysaccharide and utilization of black fungus, in particular to a method for separating and preparing polysaccharide with anti-tumor activity from black fungus. Background technique [0002] Black fungus is a nutrient-rich edible fungus, which not only has significant anticoagulant and prevents the deposition of cholesterol on blood vessels, and prevents thrombosis, but also Chinese medicine has confirmed that black fungus has a good anti-tumor effect. According to literature reports, black fungus The anti-tumor effect of fungus has a lot to do with the presence of black fungus polysaccharides. At present, the separation method of black fungus polysaccharide mainly adopts column chromatography (axial chromatography) method, and the separation of black fungus polysaccharide with anti-tumor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P35/00
Inventor 宋广磊王婧波杜琪珍
Owner ZHEJIANG GONGSHANG UNIVERSITY
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