43 results about "Photobacterium phosphoreum" patented technology

Methods for the isolation and identification of a toxicant in a sample are disclosed. Luminescent biological agents (i.e., bacteria) having sensitivity to a toxicant or an isolatable component in a sample are used to provide visually discernable zones of luminescent inhibition in the presence of a toxicant (or) in the presence of an isolatable sample component as separated by paper or thin layer chromatography. Kits for use in conjunction with the identification of a toxicant in a sample are also described, which include a luminescent biological reagent as the visualizing agent. Particular examples of luminescent bacterial agents useful in the practice of the present invention include photobacterium leoganthi, photobacterium phosphoreum, Vibrio fischeri, Vibrio harveyi a luminescent fungi, a luminescent fish extract, a luminescent dinoflagellate and fluorescent microorganisms, such as Cypridina. Potential toxicants in a liquid sample, a solid sample, or in a gaseous sample may be identified and further chemically characterized using the described methods. The isolation of potential toxicants in a sample through the processing of a sample through a separation phase matrix such as chromatography paper or TLC plate, followed by exposure to luminescent biological agent, provides for a rapid and inexpensive method for identifying pesticides, herbicides and heavy metals in a known or unknown sample.

The invention discloses a strain of photobacterium Hb1029 and its application. Experiments have proved that the photobacterium Hb1029 of the present invention is effective against various plant fungi such as banana wilt, tomato wilt, guava branch blight, pitaya fruit rot, and citrus. Canker and other plant bacterial pathogens have shown good inhibitory effect; meanwhile, Photobacterium Hb1029 has growth inhibitory or insecticidal activity against plant pests such as beet armyworm, Bactrocera dorsalis, and citrus leaf miner. The biocontrol bacterium has insecticidal and bacteriostatic effects, and has a wide control spectrum. Moreover, the insecticidal and antibacterial effects of the bacterial strain are stable after repeated subculture. It has a good fresh-keeping effect when used for fruit fresh-keeping, and has good safety at the same time, and has important economic significance.

The invention discloses a mermaid Photobacterium hemolysin Hly with immune protection effect ch protein, the present invention constructs the recombinant vector pMD18T-hly ch , recombinant expression vector pET32a‑hly ch and prokaryotic expression hly ch Gene, preparation of recombinant Hly ch Protein, and use SDS-PAGE analysis and Western blot to verify Hly ch protein, and animal protection experiments to evaluate its protective power. Hly of Photobacterium mermaid obtained in the present invention ch Protein has good immune protection. It laid the foundation for the development of the genetically engineered subunit vaccine of Photobacterium mermaidus.

The invention provides a preparation method of photobacterium damsela vaccines of cynoglossus semilaevis, which is characterized by comprising the steps of: inoculating ST1 strains of photobacterium damsela on an ordinary nutrient agar bouillon culture medium, cultivating for 24h at the temperature of 28 DEG C, and adding formaldehyde with the final concentration of 0.3% into the culture medium to inactivate the photobacterium damsela for 48h at the temperature of 28 DEG C so as to obtain inactivated bacteria; soaking milkvetch roots with distilled water, and then decocting, filtering and concentrating to obtain milkvetch lixivium; and mixing the inactivated bacteria and the obtained milkvetch lixivium to prepare the photobacterium damsela vaccines of the cynoglossus semilaevis. In the invention, the vaccines have the advantages of enabling the cynoglossus semilaevis to produce good immune response, enhancing the immunity of the cynoglossus semilaevis to the pathogen (photobacterium damsela), and increasing the survival rate of cultivated cynoglossus semilaevis, the output of the cultivated cynoglossus semilaevis, and the economic benefit of the cultivation and production of the cynoglossus semilaevis. The problem that the cynoglossus semilaevis is easy to catch diseases caused by the photobacterium damsela during the cultivation and production process at present is fundamentally solved. The invention also discloses an application method of the photobacterium damsela vaccines of the cynoglossus semilaevis.

The invention belongs to the technical field of mariculture disease prevention and control, and in particular relates to an RPA reaction system suitable for rapid detection of Photobacterium mermaid subspecies mermaid. Specific primers were designed to carry out the amplification reaction of recombinase polymerase, and after gel electrophoresis verification of the product, it was judged qualitatively whether the detection sample contained Photobacterium mermaida subsp. The invention provides a stable, specific and highly efficient Recombinase Polymerase Amplification (RPA) reaction system of Photobacterium mermaidi subsp. The research on RPA detection technology of Photobacterium mermaid subspecies has laid a technical foundation for the accurate and rapid detection of RPA of Photobacterium mermaid subspecies.

The invention discloses an application of an extracellular protein EF-Tu of Photobacterium mermaidi with immune protection function in the preparation of a subunit vaccine of Photobacterium mermaidi, wherein the preparation method of the extracellular protein EF-Tu of Photobacterium mermaidi comprises pMD18-T Construction of ‑EF‑Tu recombinant vector, construction of plasmid pET32a‑EF‑Tu, inducible expression of extracellular protein EF‑Tu of Photobacterium mermaida and verification of extracellular protein EF‑Tu of Photobacterium mermaidi. The present invention provides an extracellular protein EF-Tu of Photobacterium mermaidi with immune protection, which is expressed and purified by a prokaryotic expression system, and the immune protection of the extracellular protein EF-Tu of Photobacterium mermaidi is evaluated through animal experiments. It lays the foundation for the further development of photobacteria mermaid subunit vaccine and nucleic acid vaccine selection.

The present invention relates to a chondroitin sulfate lyase and its encoding gene and application. The chondroitin sulfate lyase enCSase in the present invention is isolated from Photobacterium sp. QA16, the amino acid sequence is shown in SEQ ID NO.2, and the nucleotide sequence of the encoding gene is shown in SEQ ID NO.1. The chondroitin sulfate lyase enCSase in the present invention is a new type of CSase ABC enzyme, which has high enzymatic activity on chondroitin sulfate, dermatan sulfate and hyaluronic acid, and degrades chondroitin sulfate, dermatan sulfate and hyaluronic acid. It is in endonuclease mode; it can be used in the preparation of chondroitin sulfate oligosaccharide, dermatan sulfate oligosaccharide and hyaluronic acid oligosaccharide, and has broad application prospects in the fields of medicine and food.

The invention belongs to the technical field of detection of environmental pollution, and discloses a method for detecting the toxicity of luminescent bacteria. The method comprises the following steps of: 1, activating strains, namely dissolving freeze dried powder of photobacterium phosphoreum, inoculating on a slant and generating the strains for 3 times for later use; 2, culturing the strains, namely inoculating the generated strains to a liquid nutrient medium by using an inoculating loop and culturing for 12 hours at the temperature of 20 DEG C and at 180rpm; 3, balancing bacteria liquid, namely adding an appropriate amount of the bacteria liquid into 20 milliliters of NaC1 solution at the concentration of 3 percent, controlling light value measurement ranges to be between 0.5 and 5million, and agitating for 40 minutes at the temperature of 20 DEG C; 4, adding samples and detecting luminous intensity, namely adding the bacteria liquid which is agitated for 40 minutes into 200 to 800 microliters of NaC1 blank solution at the concentration of 3 percent and 800 microliters of NaC1 polluted solution at the concentration of 3 percent by using a continuous sample injector, immediately and uniformly mixing for 2 minutes on a vortex mixer, standing for 15 minutes at the temperature of 20 DEG C, and placing a sample in a fluorescence detector to detect the luminous intensity; and 5, calculating the suppression ratio of the sample to the luminous intensity by a double alternate comparison method. The method has the characteristics of convenience and high precision.

The invention discloses photobacterium phosphoreum freeze-dried powder and a preparation method thereof. The photobacterium phosphoreum freeze-dried powder comprises the following components in percentage by weight: 25-35 percent of skimmed milk, 20-24 percent of mycose, 0.7-1.0 percent of arginine, 1-1.5 percent of sodium chloride and the balance of thalli. The photobacterium phosphoreum freeze-dried powder adopts a compound cryoprotectant; and experiments prove that the compound cryoprotectant is greatly improved in freezing resistance, has a higher protective property than a simplex protective agent and has good stability. The average value of luminous intensity of the photobacterium phosphoreum freeze-dried powder provided by the invention is about 1,843mv and approximate to a model expected value 1,820. When the toxicity of ZnSO4.7H2O, K2Cr2O7 and C6H5OH is detected with photobacterium phosphoreum freeze-dried powder prepared in different batches, the results show that the EC50 value variation coefficients detected by the photobacterium phosphoreum freeze-dried powder prepared in different batches are less than 6%.