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Luminous bacteria and application thereof in detecting general biological toxicity in food or water sample

A technology of food and bacteria, applied in the field of luminescent bacteria and its application in the overall biological toxicity detection of food or water samples, can solve the problems of reducing the scope of application, doubting the authenticity and reliability of toxicity assessment, and achieving broad application Scope, realistic evaluation, effects of far-reaching application prospects

Inactive Publication Date: 2009-10-21
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, from an application point of view, when the luminescent bacteria method is used for detection, the addition of such a high salt concentration will affect the properties of the sample to be tested and change, thus casting doubt on the authenticity and reliability of its toxicity assessment
Generally speaking, when using luminescent bacteria to detect toxic and harmful substances, the temperature requirements are relatively strict, and they must be near the optimum growth temperature of bacteria. Obviously, this will reduce its scope of application.

Method used

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  • Luminous bacteria and application thereof in detecting general biological toxicity in food or water sample
  • Luminous bacteria and application thereof in detecting general biological toxicity in food or water sample
  • Luminous bacteria and application thereof in detecting general biological toxicity in food or water sample

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] Obtained Photobacterium CCTCC M 208027

[0094] Put 5.00g of deep-sea silt from the sea area of ​​Ganyu in Haizhou Bay, place it in 100ml basal medium, incubate at 30℃, 200 rpm for 2 days, insert 1% of the inoculum into the same medium for passage 3 times. Then take 0.5ml of culture medium and dilute it gradually, take 10 -4 -10 -7 Each 0.1 ml of the diluted liquid was spread on a basal medium plate, cultured at 30°C for 2 days, and then observed in a dark room to pick out fluorescent colonies. They were respectively inoculated into basal salt culture test tubes, cultured in a shaker at 30° C. and 200 revolutions per minute for 24 hours, and their luminescence effects were detected with a chemiluminescence instrument.

[0095] The medium formula is:

[0096] Yeast extract 5.00g

[0097] Tryptone 5.00g

[0098] Sodium chloride NaCl 9.00g

[0099] Potassium chloride KCl 0.50g

[0100] Calcium chloride CaCl 2 ×2H 2 O 1.00g

[0101] Magnesium chloride MgCl 2 ×6H 2 O 3.00g

[01...

Embodiment 2

[0113] Preparation of Photobacterium CCTCC M 208027 Lyophilized Powder

[0114] The Photobacterium CCTCC M 208027 culture solution cultivated in Example 1 to the mid-log phase of growth was immediately placed on an ice water bath to perform the following operations:

[0115] Centrifuge for 5 minutes at 4℃, 15000g / min, discard the supernatant, resuspend once with 10ml of basic culture medium (slowly pipette evenly with a micropipette or dropper), and then centrifuge at 15000g / min at 4℃ After 5 minutes, discard the supernatant and use a freeze dryer to make it into a lyophilized powder of Photobacterium CCTCC M 208027.

[0116] The resulting solid powder is the finished product of Photobacterium CCTCC M 208027 freeze-dried powder.

Embodiment 3

[0118] Application of Photobacterium CCTCC M 208027 in the biological toxicity assessment of mixed toxicity simulation samples

[0119] The test simulation prepared contains a variety of toxic and harmful substances (the concentration of each toxic substance is IC 50 ) Mixed water samples H1, H2, H3, the types of toxic and hazardous substances contained in each sample are 2, 4, and 6 respectively (prepared according to Table 1, "+" represents the added type), and the water samples H1, H2, and H3 were diluted with concentration gradients (2 times, 4 times, 6 times, 8 times, 10 times), and then the Photobacterium CCTCC M 208027 obtained in Example 1 was used to detect the response to different samples and samples of different concentrations. Happening. A 50% inhibition of luminescence value is used as the toxicity criterion. See the result Figure 6 .

[0120] Table 1 Types of toxic and hazardous substances added to mixed water samples

[0121] Hg

As

Cr 6+

...

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PUM

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Abstract

The invention discloses luminous bacillus CCTCC M 208027 and application thereof in detecting general biological toxicity in a food or water sample. The invention also discloses a kit that contains luminous bacillus CCTCC M 208027 and is capable of detecting general biological toxicity in the food or water sample.

Description

Technical field [0001] The field of poison detection of the present invention particularly relates to a luminescent bacteria and its application in the detection of overall biological toxicity of food or water samples. Background technique [0002] Food safety is an issue that humans can never ignore. With the continuous improvement of people’s living standards, food-borne diseases caused by heavy metals, pesticide residues, antibiotics and hormones, toxins produced by spoiled food, migration of packaging materials and other toxic and harmful substances contaminating food have become a concern for food safety in today’s society. Focus. There are many reasons for the contamination of food by toxic and harmful substances, including external factors, such as spraying pesticides in the production process of agricultural products, adding auxiliary materials during food processing, etc.; there are also internal factors, such as chemical reactions during food processing; and water. , Ai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12Q1/02
Inventor 翟琦巍洪源范陈中建
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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