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Multiplex PCR detection method of pathogenic bacteria of fish sarcoidosis

A technology for sarcoidosis and pathogenic bacteria, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of long detection cycle of pathogenic bacteria and few types of one-time detection, saving labor cost and time cost, increasing Detected throughput, highly specific effects

Pending Publication Date: 2020-03-13
SHENZHEN INST OF GUANGDONG OCEAN UNIV +1
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Problems solved by technology

[0006] In order to make up for the deficiencies of the prior art and solve the problems of long detection period of pathogenic bacteria and few types of one-time detection in the prior art, the present invention provides a primer set and method for simultaneously detecting six kinds of fish sarcoidosis pathogenic bacteria
Through agarose electrophoresis, it is possible to determine which pathogenic bacteria caused sarcoidosis in fish

Method used

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  • Multiplex PCR detection method of pathogenic bacteria of fish sarcoidosis
  • Multiplex PCR detection method of pathogenic bacteria of fish sarcoidosis
  • Multiplex PCR detection method of pathogenic bacteria of fish sarcoidosis

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Embodiment Construction

[0043] The following examples can enable those skilled in the art to understand the present invention more fully, but do not limit the present invention in any way.

[0044] The present invention provides a primer set and method for simultaneously detecting six kinds of sarcoidosis pathogenic bacteria in multiplex PCR detection, and the specific scheme is as follows:

[0045]Genomic DNA was extracted from Aeromonas hydrophila, Photobacterium mermaidii subsp. piscicida, Nocardia asteratus, Aeromonas schutti, Nocardia amberii, and Nocardia salmonicida.

[0046] The design and screening of primers, the primers are as follows:

[0047] Aeromonas hydrophila upstream primer, its nucleotide sequence is shown in SED ID NO.1,

[0048] Aeromonas hydrophila downstream primer, its nucleotide sequence is shown in SED ID NO.2,

[0049] The upstream primer of Photobacterium mermaidus subsp. piscida, the nucleotide sequence of which is shown in SED ID NO.3,

[0050] The downstream primer o...

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Abstract

The invention relates to a PCR detection method of pathogenic bacteria of fish sarcoidosis, comprising the following steps of: extracting genomic DNA of Aeromonas hydrophila, Photobacterium damselae subsp. piscicida, Nocardia asteroids, Aeromonas schubertii, Nocardia seriolae and Nocardia salmonicida; carrying out primer design on the genomic DNA of pathogenic bacteria; and carrying out amplification and detection on the genomic DNA of Aeromonas hydrophila, Photobacterium damselae subsp. piscicida, Nocardia asteroids, Aeromonas schubertii, Nocardia seriolae and Nocardia salmonicida. Various pathogenic bacteria of sarcoidosis can be detected by the method, and the method has high specificity and sensitivity.

Description

technical field [0001] The invention relates to the field of gene detection, more specifically, to a method for detecting fish sarcoidosis pathogenic bacteria by multiplex PCR. Background technique [0002] Fish sarcoidosis is a common and frequently-occurring disease in aquaculture. The main symptom is white nodules in internal organs, and the histopathological diagnosis is granuloma. This disease course of disease is long, and infection speed is fast, and mortality rate is as high as 100%, and summer and autumn season is the peak period of morbidity, and water temperature is prone to fall ill when 25~27 ℃. It mainly harms more than 20 kinds of commercial fish such as snakehead (Ophiocephalus argus), large yellow croaker (Larimichthys crocea), perch (Lateolabrax japonicus), and pomfret (Trachinotus ovatus). The disease often breaks out in my country's inland and southeast coastal areas, causing huge economic losses to the aquaculture industry. [0003] Epidemiological sur...

Claims

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Application Information

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IPC IPC(8): C12Q1/689C12Q1/686C12Q1/04
CPCC12Q1/689C12Q1/686C12Q2600/16C12Q2537/143
Inventor 夏洪丽夏立群鲁义善程俊樊慧敏张子雯陈文捷龙梦喻大鹏
Owner SHENZHEN INST OF GUANGDONG OCEAN UNIV
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