41 results about "Indicator organism" patented technology

The invention discloses a water quality monitoring method based on motion features of an aquatic organism on a picture. A carassius auratus is taken as an indicator organism, two cameras are employed to shoot a swimming picture sequence of the carassius auratus in a fish tank and mean motional velocity, steering number of times, average angle of turning, average inclination angle of the body and time for staying at the upper part of water in a unit time are solved according to the picture. Water quality is automatically judged according to the unusual changes of the five indicators so as to alarm when wastewater is detected to have an exceeding concentration. The method comprises the following steps of: firstly, employing two cameras to shoot the swimming picture sequence of the carassius auratus in the fish tank, detecting target area information of the carassius auratus on the pictures by applying an image segmentation technology in two picture sequences, solving positions and angles of the carassius auratus on the frame pictures according to the target area information, then solving the mean motional velocity, the steering number of times, the average angle of turning, the average inclination angle of the body and the time for staying at the upper part of water in a unit time according to the positions and angles information of the carassius auratus on all the frame images and judging pollution degree of the water according to the five indicators finally. The water quality monitoring method based on the motion features of an aquatic organism on a picture has the advantages of simplicity and easy implementation and solves the problems of the unicity of physical and chemical testing in water quality monitoring and the like.

The invention discloses an early warning method for burst pollution of source water through aquatic organism behavior image extraction, which comprises the following steps of: ensuring that raw water to be monitored continuously passes through a monitoring container with an indicating organism by using a pump, continuously and automatically monitoring the behavior of an aquatic organism in the monitoring container by using a charge coupled device (CCD) camera, acquiring and storing an activity state data sequence of the aquatic organism with time by combining an image identification system and using a computer, processing to obtain related parameters such as an activity state average activity speed, an average activity height, an average interval, average turn times and the like of the aquatic organism, establishing a corresponding database, and comprehensively comparing behavior change data under the condition of different polluted water quality with behavior data under the blank condition to realize accurate and real-time early warning. The acquired data can be transmitted, stored and analyzed in real time, an alarm is automatically given by a communication device, and the method has the characteristics of quick response, high sensitivity and real-time, on-line and in-situ early warning.

The invention discloses an in-situ real-time intelligent biological detection device for protecting the river health. The device comprises an electronic cabin, a sampler mounted below the electronic cabin, and a sampling net mounted below the sampler, wherein a river water power and water quality information real-time acquisition device is mounted above the electronic cabin; a storage battery, a motor, a high-pressure pump, a microprocessor and a data memory card are mounted in the electronic cabin; a high-pressure water gun connected with the high-pressure pump is mounted on the inner wall of the sampler; an image identification system controlled by a microprocessor is mounted at the top of the sampler; the bottom of the sampler is in an open structure and is connected with the sampling net through a traction rope; the sampling net comprises a side net capable of stretching and contracting up and down and a turnable bottom net nested at the bottom of the side net. The invention further discloses an in-situ real-time intelligent biological detection method for protecting the river health, and the variety, the number and the size information of indicator organisms on an in-situ riverbed of a river can be continuously, automatically and intelligently detected on the premise of not destroying a river bottom substrate ecological environment.

The invention provides a river ecological flow determining method with the benthos diversity as a target. By means of the method, the research methods of ecological water requirements are enriched, and meanwhile, the ecological flow determining method with benthos as indicator organisms is provided. The method comprises the steps that river segment selection is researched; the benthos in a river segment are researched and ecological environment elements are inspected on site; the type of a benthos habitat and the characteristic parameter threshold value of the benthos habitat are determined; the optimal type of the benthos habitat which maintain the benthos diverse is determined; the fluvial geomorphology measurement of the river segment is researched; a two-dimensional hydrodynamics modelis constructed; the benthos habitat of the river segment is simulated in different flows; the optimal flow for maintaining the benthos diverse is determined. The method has the advantages that the ecological flow which enables the benthos diversity to be highest can be very accurately obtained, and the problem that by means of a general ecological flow determining method, the result cannot reflect the actual demands of aquatic organisms is solved.

A biological sensor based system for detecting odors, which comprises (a) a compartment for completely or partially secluding and enclosing a biological sensor located therewithin that is configured to enable the biological sensor to perform normal animal motions as well as an odor detection operation; (b) an air inlet in the compartment through which odor laden flows into an interior of the compartment; (c) an air outlet through which the odor laden air is dischargeable from the compartment; (d) an animal depressible button which is movably connected to a wall of the compartment and is depressible by the biological sensor upon detection that the introduced odor is a predetermined target odor; (e) a force sensor connected to the button for sensing the pressing parameters applied by the biological sensor onto the pushbutton; (f) an analysis unit for determining whether the pressing parameters are within an expected range for the biological sensor and for transmitting an alert signal to a law enforcement entity or relevant official authorities when the pressing parameters are within the expected range and the pressing parameters are indicative of a known reaction of the biological sensor after detecting the predetermined target odor.

A process challenge device tailored to mimic the resistance of a particular product to a particular biological inactivation, disinfection, or sterilization process, and used to challenge the process, thus providing a means to validate the efficacy of the process. The process challenge device is used by subjecting the device containing indicator organisms to an inactivation or sterilization process, and culturing any surviving indicator organisms as a means to assess the efficacy of procedures for the inactivation of microorganisms. The device uses a self-containing biological indicator (SCBI) with a biological indicator and media ampule located within a plastic vial. By altering the materials and / or configuration of the SCBI a wide range of resistances to sterilant gas processes may be achieved.

The invention relates to application of adult hermetia illucens serving as an indicator organism for nonuse of pesticide in organic agriculture and a species breeding method of the adult hermetia illucens, and provides a symbiosis species breeding method of the adult hermetia illucens and organic vegetables in a vegetable greenhouse. The reconstructed vegetable greenhouse is adopted, and the method comprises the following steps that 1, high temperature greenhouse closing is conducted on planting land, and insecticidal and germicidal treatment are conducted; 2, vegetables are planted, and feathered adult hermetia illucens is put in the vegetable greenhouse for common species breeding; 3, artificial control and trapping lamp and trapping board trapping and killing are conducted on pests in the breeding process; 4, an ultraviolet lamp and an ozone machine are adopted for disease control in the species breeding process. The mode of combining adult hermetia illucens breeding and vegetable planting in the vegetable greenhouse is used for breeding adult hermetia illucens breeding and planting vegetables, and on one hand, the vegetable greenhouse only needs to be subjected to simple remodification to prevent the adult hermetia illucens from flying away; on the other hand, a difference between the living temperature and humidity of the adult hermetia illucens and the living temperature and humidity of vegetable planting is small, and symbiosis of the adult hermetia illucens and the vegetables can be achieved.

The biocontainer of the present invention provides a low cost, simple solution of many of the problems encountered during shipping, freezing and thawing of biopharmaceutical materials. The present invention enables a user to monitor the temperature profile of each biopharmaceutical container during the cryogenic process, so as to ensure the integrity of materials within each biocontainer by using a pre-installed and pre-sterilized temperature sensor. In some embodiments, the sensor assembly includes a wireless transmitter and is capable of transmitting information regarding the measured reading. In other embodiments, the sensor assembly includes a processing unit, which determines whether the temperature profile is acceptable. In a further embodiment, an indicator is included, such that the processing unit may indicate whether the biopharmaceutical material has been properly frozen. In other embodiments, the sensor assembly also includes a storage element, which is capable of storing various parameters during the freezing process.

The invention discloses a biological monitoring system and a biological monitoring method for monitoring a sudden change of high-turbidity and low-temperature water quality. The system is characterized by comprising a water pretreatment system, a signal acquisition and analysis system and a signal transmission system; by internally-designed turbidity-removing and temperature-raising measurements, the technology and the system can ensure that the monitored water is a normal living environment for an indicating creature under a high-turbidity and low-temperature condition; on a basis of detecting and analyzing biologic behavior, on-line monitoring for comprehensive toxicity of the water can be realized; and according to a built-in standard change trend of the biologic behavior, the behavior change of the indicating creature is analyzed, the biologic behavior in the monitored water is ensured to be true, the change condition of the water quality is accurately represented, and the requirements of different monitoring departments for a stable long-time monitoring are met.

Methods and systems (301) are provided to predicting premature birth condition in a subject. The method for predicting or monitoring premature birth condition in a subject comprises processing a biological sample obtained from the subject to generate data indicative of a distribution of a plurality of populations of microbes of different types in the biological sample. A presence, absence, or relative amount of an individual population of the plurality of populations of microbes may be indicative of a premature birth condition. Next, a trained algorithm may be used to process the data to determine a presence, absence, or relative amount of the individual population of microbe. Next, based on the presence, absence, or relative amount, the subject may be identified as having the premature birth condition, such as, for example, in a report.

The invention belongs to the field of environmental protection, and discloses a compatibility method for acquiring an optimal combined biological effect by testing a binary mixture. The method comprises the following steps: (1) the testing method is based on GB / T 15441-1995, and photobacterium phosphoreum is used as an indicator organism to test the single toxicity EC50-A and EC50-B of A and B compounds; (2) according to the EC50-A and the EC50-B, a mixed solution with an equivalent effect ratio is prepared by a testing method which is based on GB / T15441-1995, the biological effect concentration of the mixed solution is tested to obtain the concentration CA and CB of A and B in a mixed system when inhibition of the mixed system to the biological effect is 50 percent, and a combined biological effect index TU1:1 is calculated in the equivalent effect ratio; and (3) a combined biological effect regulation of the binary mixed system can be predicted according to the testing result TU1:1 of the combined biological effect, which is calculated in the equivalent effect ratio, and the optimal biological effect point can be acquired. The method can be applied in the aspects of environmental science, medication, pesticide and the like.

The invention relates to a real-time earth surface water body water quality monitoring method based on biological motion trail change. The monitoring method comprises the following steps of: 1, arranging a first monitoring point (19) and a second monitoring point (20), and arranging a first automatic sample storage bottle, a second automatic sample storage bottle, a third automatic sample storagebottle and a fourth automatic sample storage bottle through pipelines; 2, taking a water sample into the first automatic sample storage bottle (9) and the third automatic sample storage bottle (11), and automatically rotating for storage; 3, taking biological monitoring samples into the second automatic sample storage bottle (10) and the fourth automatic sample storage bottle (12), and feeding thesample into a biological monitoring vessel (15) of planktonic indicator organisms; 4, arranging a high-definition camera (22) relative to the biological monitoring vessel (15), tracking plankton (18)through the high-definition camera (22), and recording the movement condition of the plankton; 5, performing contrastive analysis according to the plankton motion trail diagram so as to monitor the water quality condition of the water body in real time; and 6, determining physical and chemical indexes of laboratory analysis according to the water quality change indicated by the plankton.

The invention belongs to the technical field of environmental engineering, and particularly relates to a method for quickly starting an A2 / O system by utilizing dredged sediment and a judgment methodfor successful starting. Dredged sediment is taken and subjected to stuffy aeration for 1-3 days, the stuffy-aerated sediment is added into an A2O+MBR reactor, the dissolved oxygen content of each tank is controlled, and a nutrient solution is continuously fed. The methods have the characteristics of resource utilization, treatment of waste with waste, high pollutant removal rate and excellent sludge performance. Continuous culturing is conducted for 5 days or more, sludge water in an aerobic tank is taken for 2-3 times every day, indicator organisms are observed under a microscope, the numberof immobilized ciliates and rotifers is recorded, the average number of the immobilized ciliates and the average number of the rotifers in 1ml of the sludge water in the aerobic tank are calculated,and when the number of the immobilized ciliates is continuously more than or equal to 800 for three times and the number of otifers is more than or equal to 45 and less than or equal to 150, it is indicted that the reactor is started successfully. The judgement method can rapid and directly reflect the change of the environment and the starting process of the reactor, and the process is guided intime.

The invention discloses a method for treating black and odorous water through a combination of an indigenous microbial film and microalgae. The method comprises the following steps: a pretreatment process of biological contact oxidation and co-cultivation of the indigenous microbial film: pretreating black and odorous water through a contact oxidation method, performing co-cultivation of indigenous microorganisms at the same time, and conducting a series of biochemical reactions such as nitrosation, nitrification and denitrification under the action of rich microorganisms so as to finally forma nitrogen-separated water body, thereby reducing the content of ammonia nitrogen in the water body and improving the quality of the water body; and microalgal enhancement treatment: carrying out microalgal enhancement treatment on pretreated effluent so as to realize purification and reutilization of the black and odorous water. According to the method, the black and odorous water is pretreatedby virtue of contact oxidation and co-cultivation of the indigenous microbial film, and then ultraviolet-induced Haematococcus pluvialis M1 is inoculated to further purify the water; and due to the characteristic that the Haematococcus pluvialis can be converted into red hard-shell spores from green vegetative cells under stress conditions (strong light and nitrogen deficiency), the Haematococcuspluvialis can be used as an indicator organism for verifying the treatment degree of the black and odorous water.

The invention discloses a method for monitoring and evaluating environment quality of Laizhou Bay marine ranching on the basis of living body response. Four key species of different living habits areselected as biological indicators from Laizhou Bay marine ranching, one biomarker of gene expression or enzyme activity levels is selected from target tissue of each biological indicator, various types of biomarker data are integrated by using a multi-biomarker pollution index MPI method, and monitoring and evaluation on biomarkers of multiple species at multiple levels are achieved. Defects of indirectness, secondary pollution and the like of conventional chemical detection can be overcome; hysteresis of biological community diversity detection can be overcome; and the method is an effectiveand sensitive biological monitoring method, in addition, the method is low in professional requirement on operators, rapid in detection speed and low in cost, and technical support can be provided forearly warning and biological monitoring system establishment for marine ranching environments.

The invention relates to a method that monitors the combined pollution of heavy metals and polycyclic aromatic hydrocarbon in soil in the technical field of environmental protection, which comprises the following steps: step 1. indicator organism is contaminated; step 2. an earthworm coelomocyte is extracted; step 3. the cell to be measured is collected; step 4. the cell to be measured is washed; step 5. the cell to be measured is dyed; step 6. the survival rate and the early apoptosis rate of the cell are measured; and step 7. the combined pollution degree of the heavy metals and the polycyclic aromatic hydrocarbon in the soil is determined according to the degree of the survival rate and the early apoptosis rate of the cell. The method determines the combined pollution degree of the heavy metals and the polycyclic aromatic hydrocarbon in the soil by analyzing the survival rate and the early apoptosis rate of the earthworm coelomocyte, thus having sensitivity, high efficiency, simple and convenient operation and good repetitiveness, being particularly applicable to the detection of the combined pollution situation of the heavy metals and the polycyclic aromatic hydrocarbon in the soil in old industrial bases, and having good early warning action to the combined pollution of the heavy metals and the polycyclic aromatic hydrocarbon in the soil.

The disclosure relates to a method and a system for determining the fertility status of a female and / or the status of a biomarker in a mammal or human being. A method the method comprises determining, for a plurality of first time intervals, a continuous series of temperature data points relating to a body temperature. The method further comprises determining, in the series of data points, for each one of one of the plurality of first time intervals, a difference between a temperature maximum and a temperature minimum to obtain a plurality of temperature amplitudes. A series of temperature amplitudes is determined from the plurality of temperature amplitudes and determining if a first portion of the series of temperature amplitudes varies from a second portion of the series of temperature amplitudes, the variation indicating a change in the status of the biomarker.

The invention belongs to the technical field of biological monitoring and evaluation of antibiotic environmental pollution, and particularly relates to a method for detecting and evaluating the environmental risk of sulfamethoxazole by using a paramecium biomarker and IBR, wherein the environmental concentration and the environmental risk of sulfamethoxazole are rapidly evaluated by various biomarkers of sensitive indicator organisms and IBR integrated analysis.

The invention discloses a method for jointly treating black and odorous water by using indigenous microbial membranes and microalgae, including a pretreatment process for co-cultivating indigenous microbial membranes by biological contact oxidation: pretreating black and odorous water through contact oxidation, and simultaneously cultivating Indigenous microorganisms, under the action of abundant microorganisms, through a series of biochemical reactions such as nitrosation, nitrification and denitrification, finally form nitrogen out of the water body, thereby reducing the ammonia nitrogen content in the water body and improving the water quality; microalgae enhanced treatment: the pretreatment The final effluent is treated with microalgae intensified treatment, which can realize the purification and reuse of black and odorous water; the black and odorous water is pretreated by contact oxidation and co-cultured with indigenous microbial membranes, and then inoculated with UV-induced Haematococcus pluvialis M1 for further purification Water quality: Haematococcus pluvialis can be transformed from green vegetative cells into red hard-shelled spores under stress conditions (strong light, nitrogen deficiency), making it an indicator organism for verifying the treatment degree of black and odorous water.

The invention provides a direct-viewing, accurate and practical combined application method of 'water body sampling, bionidicator, water environmental simulation and watershed health diagnosis' for diagnosing health of a watershed ecology system aiming at the problem that watershed pollution affects the health of the ecology system and dominant species and pollutants are different in different watershed biology systems. The method comprises the following steps of: firstly, rating the dominant species of the watershed biology system, and analyzing types of key pollutants of the watershed so as to select a biological indicator; secondly, designing a water environment simulation area according to the similarity law; thirdly, determining a watershed sampling point, setting a water intake so as to introduce watershed water body into the water environment simulation area, and planting and / or breeding the biological indicator in the water environment simulation area; and finally, draining the wastewater in the simulation area into the watershed water body by using a discharging system. By adopting the method, the health of the watershed biology system is determined by observing the health condition of the biological indicator in the simulation area, and the method has high scientificity and practicality, is suitable for health diagnosis of the watershed biology systems which are in different scales and contain different pollutants, and has the advantages of direct viewing and accurate determination.

The long afterglow immunochromatographic test strip includes a bottom plate and a sample area, a detection area and an adsorption area which are sequentially connected on the bottom plate; the sample area contains long afterglow nanospheres coupled with the first biomarker; the long afterglow nanospheres Including polymer microspheres and long-lasting luminescent materials embedded in the polymer microspheres; the first biomarker can specifically bind to the target of the new coronavirus contained in the test sample; A detection line and a quality control line are set; the detection line is sprayed with a second biomarker that can specifically bind to the target to be detected; the quality control line is sprayed with an activity verification substance; the activity verification substance is used to indicate the effectiveness of the biomarker . The long afterglow immunochromatographic test strip has the advantages of convenient detection, high sensitivity, accurate test, low cost and easy promotion.

The invention belongs to the technical field of biological monitoring and evaluation of antibiotic environmental pollution, and particularly relates to a method for detecting and evaluating levofloxacin environmental risk by using paramecium biomarkers and IBR, wherein the method is used for rapidly evaluating the levofloxacin environmental concentration and environmental risk by using multiple biomarkers of a sensitive indicator organism and IBR integration analysis.

The invention belongs to the technical field of biological monitoring and evaluation of antibiotic environmental pollution, and particularly relates to a method for detecting and evaluating tetracycline environmental risk by using paramecium biomarkers and IBR, wherein tetracycline environmental concentration and environmental risk are rapidly evaluated by using various biomarkers of sensitive indicator organisms and IBR integrated analysis.

A point of care testing (POCT) instrument (1) for analyzing biological samples and a POCT system (10) comprising a plurality of such POCT instruments (1) are proposed. The POCT instrument (1) comprises a housing (2), a measurement unit (3) designed for collecting data indicative of at least one biological parameter of a biological sample, a main display (4), and an auxiliary display (5).