Method for detecting and evaluating environmental risk of sulfamethoxazole by using paramecium biomarker and IBR
A biomarker, sulfamethoxazole technology, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of high quality, low practicability and long cycle.
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[0055] 1. Materials and methods
[0056] 1.1 Indicator organisms
[0057]Paramecia (Parameci μm ca μdat μm), Cilia, Oligomembrane, Hymenostomy, Paramecia, Paramecia genus. Widely distributed in still water bodies rich in organic matter. It is sensitive to the stimulation of environmental pollutants, so it is often used in the evaluation research of environmental pollutants.
[0058] 1.2 Cultivation of Paramecium
[0059] It can be collected in fresh water bodies rich in plankton, pick single cells, wheat grain culture medium, establish monoclonal culture lines in a constant temperature and humidity incubator at 20-25°C, starvation-induced conjugation, large-scale pure culture, and logarithmic growth Phase individuals were used for sulfamethoxazole testing.
[0060] 1.3 Setting of Sulfamethoxazole Detection Concentration Range
[0061] According to various literature reports and environmental monitoring reports, the experimental concentration of sulfamethoxazole in the wat...
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