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A kind of daphnia highly concentrated food and its preparation method

A high-concentration, flea-like technology is applied in the field of preparing flea-like high-concentration food, can solve problems such as water flea suffocation, interference of test results, etc., and achieves the effects of low price, improved accuracy, and improved culture efficiency.

Active Publication Date: 2016-06-15
武汉润江生态科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention well solves the problem of suffocation of daphnia due to the lack of oxygen in the water body at night due to the excessive addition of live algae during the cultivation process of daphnia, and also solves the problem of interference to the test results caused by the addition of live algae in the daphnia toxicity test. question

Method used

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  • A kind of daphnia highly concentrated food and its preparation method
  • A kind of daphnia highly concentrated food and its preparation method
  • A kind of daphnia highly concentrated food and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A highly concentrated food of daphnia, prepared from the following raw materials:

[0028]

[0029] The described chlorella pyrenoidosa is measured by the wet weight of the chlorella pyrenoidosa.

Embodiment 2

[0031] A preparation method of daphnia highly concentrated food, the steps are:

[0032] a. preparation of culture medium, prepare 10L algal culture medium according to table 1

[0033] Table 1 Algae medium composition list

[0034] the element

Content (mg)

CaCl 2 2H 2 o

116.0

K H 2 PO 4

1533.0

urea

5000.0

MgSO 4

2000.0

K 2 HPO 4

2400.0

Biotin

0.005

Na 2 EDTA

250.0

CoCl 2 6H 2 o

0.10

Na 2 MoO 4 2H 2 o

0.06

CuSO 4 5H 2 o

0.10

Thiamin HCL

1.0

FeCl 3 6H2O

10.0

Vitamin B12

0.005

[0035] h 3 BO 3

10.0

ZnSO 4 7H 2 o

0.22

MnCl 2 4H 2 o

1.80

[0036] b. High temperature sterilization

[0037] Seal the prepared 10L culture medium with cotton cloth, sterilize it in an autoclave at 121°C for 30 minutes, and cool it for later use.

[0038] ...

Embodiment 3

[0047] Comparative Test:

[0048] Using self-made high-concentration food for daphnia, daphnia food produced by BBE in Germany and live algae, the experiment of feeding daphnia was carried out in the laboratory to compare the growth and reproduction of daphnia.

[0049] The experiment was divided into 3 groups, namely the self-made daphnia-like high-concentration food-fed group, the German BBE daphnia-like food-fed group and the living algae-fed group.

[0050] The experiment was carried out in a 250ml beaker. At the beginning of the experiment, 100ml of diluent was added to each beaker. The preparation of the diluent was prepared according to the requirements in the national standard GB / T13266-91 "Water Quality-Materials to (Magna) Acute Toxicity Determination Method". Then add 4 water fleas, the age is 3 days after birth, keep the indoor temperature at 25°C, add 4μl concentrated solution every day, observe continuously for 11 days, record the growth and reproduction of water...

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Abstract

The invention discloses a high-concentration food of daphnia and a preparation method thereof, which comprises chlorella pyrenoidum, sodium alginate, carrageenan, xanthan gum, sodium carboxymethyl cellulose, potassium sorbate and magnesium carbonate in a certain proportion formulated. The invention solves the problem of lack of oxygen at night caused by adding too much living algae in the cultivation of daphnia, reduces the influence caused by adding living algae when the daphnia is used as an indicator organism for toxicity testing, and improves the accuracy of toxicity testing. Because the product is a highly concentrated food, only a small amount can be added when cultivating daphnia, which can greatly reduce the feeding frequency and improve the cultivation efficiency of daphnia.

Description

technical field [0001] The invention belongs to the field of water environment monitoring, and more specifically relates to the preparation of high-concentration daphnia food by using chlorella pyrenoidum, sodium alginate, xanthan gum, carrageenan, carboxymethylcellulose sodium potassium sorbate and magnesium carbonate method. At the same time, it involves the cultivation of Daphnia and the comprehensive toxicity test of Daphnia. This food is a highly concentrated food, suitable for feeding daphnia, fish, shrimp, etc., with high protein content, rich nutrition and long shelf life. technical background [0002] With the rapid development of social economy, my country's water environment problems are becoming more and more serious. At present, nearly 40% of the river sections in the country are seriously polluted. According to the data in the Water Resources Bulletin of the Ministry of Water Resources, the water quality of the national rivers from 2001 to 2011 was worse than ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033A23K50/80A23K10/10A23K10/40
Inventor 王英才李立印士勇刘辉朱圣清
Owner 武汉润江生态科技有限公司
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