Compatibility method for acquiring optimal combined biological effect by testing binary mixture
A technology that combines biological and biological effects, used in biological testing, chemiluminescence/bioluminescence, analysis by chemically reacting materials, etc.
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Embodiment 1
[0074] (1) Determination steps of single compound biological effect concentration
[0075] Add 0.25ml of Luminescent bacteria (purchased from Nanjing Institute of Soil Science, Chinese Academy of Sciences) solution (T3) into a volumetric flask containing 5ml of medium, and cultivate for 12 hours to make a shake flask bacteria solution; then take 0.2ml shake flask bacteria solution to 20ml 3% sodium chloride, aerated and stirred for 40 minutes to make a working bacteria solution. Take an appropriate amount of malononitrile and p-nitrobenzaldehyde to make a standard series of logarithmic gradients, with a volume of 0.8ml, add 0.2ml of working bacteria solution, shake well, expose to poison for 15 minutes, and measure the decrease in light value of the system before and after exposure degree, the concentration at which the inhibition rate is 50% is calculated by interpolation method, which is the EC of the substance (or system) 50 value. Calculated EC 50 values are respectiv...
Embodiment 2
[0109] (1) Determination steps of single compound biological effect concentration
[0110]Add 0.25ml of Luminescent Bacteria solution (T3) to a volumetric flask containing 5ml of culture medium, and cultivate for 12 hours to make a shake flask solution; then take 0.2ml of shake flask solution into 20ml of 3% sodium chloride and aerate Stir for 40 minutes to make a working bacteria solution. Take an appropriate amount of phthalonitrile (compound A) and p-dimethylaminobenzaldehyde (compound B) to form a standard series of logarithmic gradients, with a volume of 0.8ml, add 0.2ml of working bacteria solution, shake well, and infect for 15 minutes. Measure the reduction degree of the light value of the system before and after exposure, and use the interpolation method to calculate the concentration when the inhibition rate is 50%, which is the EC of the substance (or system) 50 value. Calculate the EC of phthalonitrile (A compound) and p-dimethylaminobenzaldehyde (B compound) 50...
Embodiment 3
[0131] (1) Determination steps of single compound biological effect concentration
[0132] Add 0.25ml of luminescent bacteria solution (T3) to a volumetric flask containing 5ml of medium, and cultivate for 12 hours to make a shake flask solution; then take 0.2ml shake flask solution into 20ml of 3% sodium chloride, Gas stirring for 40min to make working bacteria liquid. Take an appropriate amount of acetonitrile (substance A) and terephthalaldehyde (substance B) to form a standard series of logarithmic gradients, with a volume of 0.8ml, add 0.2ml of working bacteria solution, shake well, expose for 15 minutes, and measure the system before and after exposure. The reduction degree of light value, the concentration at which the inhibition rate is 50% is calculated by interpolation method, which is the EC of the substance (or system) 50 value. Calculated EC 50 The values are respectively 0.189, 6.892×10 -5 mol / L.
[0133] (2) Determination of the joint biological effect of...
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