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Method for monitoring combined pollution of soil heavy metal and polycyclic aromatic hydrocarbon

A technology for polycyclic aromatic hydrocarbons and complex pollution, applied in biochemical equipment and methods, measuring devices, and microbial determination/inspection, can solve the problems of unsuitable soil pollution monitoring and early warning, poor sensitivity, and long experiment cycle, etc., to achieve Easy to operate, good warning effect, good repeatability

Inactive Publication Date: 2009-01-14
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its disadvantages are: this method is limited to cell poisoning in vitro, and the accumulation of neutral red is used as the detection index. The experimental period is long and the sensitivity is poor. It cannot truly reflect the damage of coelomocytes under the stress of pollutants, so it is not suitable for Monitoring and early warning of soil pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Use fiber needles combined with ultrasonic stimulation to extract coelomocytes from earthworms contaminated with single Cd: robust adult earthworms with an average body weight of 300 mg and rings. Colon cleansing for 24 hours, with a concentration of 0.01mol L -1 , PBS buffer solution of pH 7.2 to wash the earthworms repeatedly, add 3mL PBS containing 2.5mM ethylene glycol-bis-(2-aminoethyl)tetraacetic acid (EGTA) to a 10mL centrifuge tube with a length of 1cm and an inner diameter of 0.5mm The hollow fiber needle tube is inserted into the body cavity of the earthworm and put into the centrifuge tube. Stimulate the earthworms quickly with an ultrasonic instrument with a frequency of 28KHz and a power of 300W for 2 seconds, and the liquid in the thin tube of the fiber needle can be seen to turn yellow quickly. Move the cells into a 50mL centrifuge tube and add 12mL of Hank’s solution to wash three times.

[0019] Collect the cells to be tested in a 10 mL centrifuge tube...

Embodiment 2

[0026] Use fiber needles combined with ultrasonic stimulation to extract coelomocytes from earthworms contaminated with single Phe: robust adult earthworms with an average body weight of 300 mg and a ring. Colon cleansing for 24 hours, with a concentration of 0.01mol L -1, PBS buffer solution of pH 7.2 to wash the earthworms repeatedly, add 3mL PBS containing 2.5mM ethylene glycol-bis-(2-aminoethyl)tetraacetic acid (EGTA) to a 10mL centrifuge tube with a length of 1cm and an inner diameter of 0.5mm The hollow fiber needle tube is inserted into the body cavity of the earthworm and put into the centrifuge tube. Stimulate the earthworms quickly with an ultrasonic instrument with a frequency of 28KHz and a power of 300W for 2 seconds, and the liquid in the thin tube of the fiber needle can be seen to turn yellow quickly. Move the cells into a 50mL centrifuge tube and add 12mL of Hank’s solution to wash three times.

[0027] Collect the cells to be tested in a 10 mL centrifuge tub...

Embodiment 3

[0033] Using fiber needles combined with ultrasonic stimulation to extract coelomocytes from earthworms polluted by Cd-Phe compound poisoning: robust adult earthworms with an average body weight of 300 mg and annulus. Colon cleansing for 24 hours, with a concentration of 0.01molL -1 , PBS buffer solution of pH 7.2 to wash the earthworms repeatedly, add 3mL PBS containing 2.5mM ethylene glycol-bis-(2-aminoethyl)tetraacetic acid (EGTA) to a 10mL centrifuge tube with a length of 1cm and an inner diameter of 0.5mm The hollow fiber needle tube is inserted into the body cavity of the earthworm and put into the centrifuge tube. Stimulate the earthworms quickly with an ultrasonic instrument with a frequency of 28KHz and a power of 300W for 2 seconds, and the liquid in the thin tube of the fiber needle can be seen to turn yellow quickly. Move the cells into a 50mL centrifuge tube and add 12mL of Hank’s solution to wash three times.

[0034] Collect the cells to be tested in a 10 mL ce...

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PUM

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Abstract

The invention relates to a method that monitors the combined pollution of heavy metals and polycyclic aromatic hydrocarbon in soil in the technical field of environmental protection, which comprises the following steps: step 1. indicator organism is contaminated; step 2. an earthworm coelomocyte is extracted; step 3. the cell to be measured is collected; step 4. the cell to be measured is washed; step 5. the cell to be measured is dyed; step 6. the survival rate and the early apoptosis rate of the cell are measured; and step 7. the combined pollution degree of the heavy metals and the polycyclic aromatic hydrocarbon in the soil is determined according to the degree of the survival rate and the early apoptosis rate of the cell. The method determines the combined pollution degree of the heavy metals and the polycyclic aromatic hydrocarbon in the soil by analyzing the survival rate and the early apoptosis rate of the earthworm coelomocyte, thus having sensitivity, high efficiency, simple and convenient operation and good repetitiveness, being particularly applicable to the detection of the combined pollution situation of the heavy metals and the polycyclic aromatic hydrocarbon in the soil in old industrial bases, and having good early warning action to the combined pollution of the heavy metals and the polycyclic aromatic hydrocarbon in the soil.

Description

technical field [0001] The invention relates to a soil pollution monitoring method in the technical field of environmental protection, in particular to a method for monitoring complex pollution of soil heavy metals and polycyclic aromatic hydrocarbons. Background technique [0002] Soil complex pollution is a hot spot in the frontier of environmental protection scientific research, but it is also a research difficulty. Due to the complexity of combined pollution and the limitations of experimental methods, there is a lack of experimental methods for quantitative characterization of combined pollution of pollutants. In recent years, earthworms have been used as carriers to test chemical substances that may cause soil environmental pollution, and the possible environmental hazards of these chemical substances have been evaluated based on their toxicity to earthworms. With the development of biotechnology at the cellular level, subcellular level, and molecular level, the detec...

Claims

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Application Information

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IPC IPC(8): G01N33/00C12Q1/02
Inventor 朱江陆贻通
Owner SHANGHAI JIAO TONG UNIV
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