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Photobacterium lyophilized powder and preparation method thereof

A technology of photobacillus and freeze-dried powder, which is applied in the direction of bacteria and preserved microorganisms, can solve the problems of poor stability of photobacillus freeze-dried powder, and achieve the goal of improving stability and activity persistence, improving stability and excellent stability Effect

Active Publication Date: 2017-12-22
深圳市朗石科学仪器有限公司
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Problems solved by technology

[0003] In order to overcome the problem of poor stability of the existing photobacteria freeze-dried powder, the present invention provides a kind of photobacteria freeze-dried powder and its preparation method

Method used

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  • Photobacterium lyophilized powder and preparation method thereof
  • Photobacterium lyophilized powder and preparation method thereof
  • Photobacterium lyophilized powder and preparation method thereof

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Embodiment Construction

[0019] In order to make the purpose, technical solutions and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the accompanying drawings and implementation examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0020] see figure 1 , the first embodiment of the present invention provides a preparation method S10 of Photobacteria freeze-dried powder, which includes the following steps:

[0021] Step S101, preparing a lyophilized suspension; mixing the photobacteria to be prepared with protective additives to form a lyophilized suspension;

[0022] Step S102, preparing and obtaining a pre-frozen product; pre-freezing the lyophilized suspension to obtain a pre-frozen product, wherein the pre-freezing temperature is 5-10°C lower than the eutectic point temperature of the lyophilized suspensio...

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Abstract

The present invention relates to photobacterium lyophilized powder and a preparation method thereof, desired photobacterium and a protective additive are mixed to form a lyophilized suspension; the lyophilized suspension is frozen to pre-freezing temperature to obtain a pre-frozen product, sublimation drying and desorption drying are in turn performed on the pre-frozen product, the sublimation drying includes at least two temperature-raising and temperature-maintaining processes, so that the pre-frozen product is gradually raised from the pre-freezing temperature to first temperature, and the first temperature is 48 DEG C-64 DEG C higher than the pre-freezing temperature. The present invention further provides the photobacterium lyophilized powder, and the photobacterium lyophilized powder is prepared by the preparation method of the photobacterium lyophilized powder. The method overcomes the problem of the poor stability of the existing photobacterium lyophilized powder, improves the storage stability of the photobacterium lyophilized powder and prolongs the shelf life of the photobacterium lyophilized powder.

Description

【Technical field】 [0001] The invention relates to the field of water quality monitoring, in particular to a freeze-dried powder of photobacteria and a preparation method thereof. 【Background technique】 [0002] With the current situation of environmental pollution in our country, water quality issues have been widely concerned. Among them, the current water quality is detected by the luminescent bacteria toxicity detection method. The luminescent bacteria toxicity detection method has the characteristics of simple detection operation and accurate detection. Since the relative luminosity of luminescent bacteria is related to the comprehensive toxicity of water quality, the monitoring of water quality can be realized by detecting the changes of sensitive luminescent bacteria. In the prior art, freeze-dried powder of luminescent bacteria is generally used for water quality monitoring. Due to the limitations of the existing freeze-dried powder process of Photobacteria, the ex...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04
CPCC12N1/04C12N1/20
Inventor 李劲松严百平顾伟平杨艳华陈尧
Owner 深圳市朗石科学仪器有限公司
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