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A Strain of Photobacterium hb1029 and Its Application

A technology of photobacteria and fermentation liquid, applied in the direction of application, bacteria, fungicides, etc., can solve problems such as food poisoning, pesticide residues, environmental pollution, etc., and achieve the effects of reduced use, low cost, and a wide spectrum of control

Active Publication Date: 2020-09-08
POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI
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  • Abstract
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Problems solved by technology

[0007] An object of the present invention is to provide a high-efficiency, broad-spectrum, and stable-effect photobacterium neophotobacterium in view of the problems of pesticide residues, food poisoning, and environmental pollution caused by the large-scale use of chemical agents in the prevention and control of agricultural diseases and insect pests and post-harvest fruit preservation.

Method used

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  • A Strain of Photobacterium hb1029 and Its Application
  • A Strain of Photobacterium hb1029 and Its Application
  • A Strain of Photobacterium hb1029 and Its Application

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Embodiment Construction

[0029] Isolation of Photobacterium Hb1029

[0030] Isolation of Photobacteria by NBTA, an obligate medium for commensal bacteria of entomopathogenic nematodes. Three healthy larvae of S. mellonella were selected, placed in a petri dish covered with sterilized filter paper, and 500 μL of entomopathogenic nematodes (concentration of 500-600 larvae / mL) were sucked and dropped on the body surface of S. mellonella, and cultured overnight at 25°C. Disinfect the surface of the Mellonella mellonella larvae infected by entomopathogenic nematodes with 75% alcohol cotton, cut off the forefeet, take the body fluid (10-20 μL) into a centrifuge tube containing 200 μL sterile water, mix well, and inoculate On NBTA medium (beef extract 5.0g, peptone 10.0g, sodium chloride 5.0g, agar 20.0g, musk bromothymol blue 0.025g, triphenyltetrazolium chloride 0.040g, water 1L) plate, After coating evenly, incubate at 28°C for 48 hours at a constant temperature, and the blue single colony that appears o...

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Abstract

The invention discloses a strain of photobacterium Hb1029 and its application. Experiments have proved that the photobacterium Hb1029 of the present invention is effective against various plant fungi such as banana wilt, tomato wilt, guava branch blight, pitaya fruit rot, and citrus. Canker and other plant bacterial pathogens have shown good inhibitory effect; meanwhile, Photobacterium Hb1029 has growth inhibitory or insecticidal activity against plant pests such as beet armyworm, Bactrocera dorsalis, and citrus leaf miner. The biocontrol bacterium has insecticidal and bacteriostatic effects, and has a wide control spectrum. Moreover, the insecticidal and antibacterial effects of the bacterial strain are stable after repeated subculture. It has a good fresh-keeping effect when used for fruit fresh-keeping, and has good safety at the same time, and has important economic significance.

Description

technical field [0001] The invention belongs to the technical field of biopesticides, in particular to a new bacterial strain and its application, in particular to a photorhabdus luminescens Hb1029 and its application. Background technique [0002] At present, the prevention and control of plant diseases and insect pests and the post-harvest preservation of fruits mainly rely on chemical pesticides. However, the extensive use of chemical pesticides will not only cause excessive pesticide residues and resistance of diseases and insect pests, but also pollute the environment and cause problems such as human and animal poisoning. Due to its low side effects, environmental friendliness, and safety to humans and animals, biopesticides are green pesticides with broad prospects and have become an important choice in biological pest control measures. [0003] Studies have shown that entomopathogenic nematodes are safe for humans and animals because they are non-toxic and non-polluti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01P3/00A01P7/04A23B7/155C12R1/01
CPCA23B7/155A23V2002/00A01N63/10C12N1/20C12N1/205C12R2001/01A23V2200/10
Inventor 赖多邵雪花匡石滋凡超田世尧庄镇瑞
Owner POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI
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