Photobacterium phosphoreum and application thereof
A bright luminous bacterium, a technology for use, applied in the direction of bacteria, lyase, microorganisms, etc., can solve the problems of low production of alginate lyase, limited application, poor storage stability, etc., easy to achieve industrial scale-up production, simple ingredients, and preparation simple effect
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Embodiment 1
[0036] A method for producing alginate lyase, the specific method is as follows:
[0037] (1) Inoculate the slant culture of Photobacterium lumina SRU-2 into the seed medium, and cultivate it at a temperature of 27.8°C and a shaker speed of 180rpm for 14-16 h to the logarithmic growth phase to obtain a seed solution. The formula of the medium is as follows: every 100 mL of seed medium contains 1.80 g of tryptone, 0.48 g of yeast extract, 2.80 g of sodium chloride, and 0.80 g of glycerin, with a natural pH;
[0038] (2) Prepare the fermentation medium according to the ratio of 30mL fermentation medium to 500mL shake flask, and transfer the seed liquid obtained in step (1) to the fermentation medium with an inoculum of 2.0%. 6.6. Cultivate for 22-26 hours at a shaker speed of 180 rpm to obtain a fermentation broth. The formula of the fermentation medium is as follows: Each 100mL fermentation medium contains 0.50g of glucose, 0.18g of peptone, 0.48g of sodium chloride, dihydrogen...
Embodiment 2
[0042] A method for producing alginate lyase, the specific method is as follows:
[0043] (1) Inoculate the slant culture of Photobacterium lumina SRU-2 into the seed medium, and cultivate it at a temperature of 28.5°C and a shaker speed of 220rpm for 14-16 h to the logarithmic growth phase to obtain a seed liquid. The formula of the medium is as follows: every 100 mL of seed medium contains 2.20 g of tryptone, 0.52 g of yeast extract, 3.20 g of sodium chloride, and 1.20 g of glycerin, with a natural pH;
[0044] (2) Prepare the fermentation medium according to the ratio of 30mL fermentation medium to 500mL shake flask, and transfer the seed solution obtained in step (1) to the fermentation medium with an inoculum of 2.0%. 7.6. Cultivate for 22~26 hours under the condition of shaker speed 220 rpm to obtain fermentation broth. The formula of the fermentation medium is as follows: every 100mL fermentation medium contains 0.70g of glucose, 0.25g of beef extract, 0.52g of sodium c...
Embodiment 3
[0048] A method for producing alginate lyase, the specific method is as follows:
[0049] (1) Inoculate the slant culture of Photobacterium lumina SRU-2 into the seed medium, and cultivate it at a temperature of 28.0°C and a shaker speed of 200rpm for 14-16 h to the logarithmic growth phase to obtain a seed liquid. The medium formula is as follows: Each 100mL seed medium contains tryptone 2.00g, yeast extract 0.50g, sodium chloride 3.00g, glycerin 1.00g, and the pH value is natural;
[0050] (2) Prepare the fermentation medium according to the ratio of 30mL fermentation medium to 500mL shake flask, transfer the seed solution obtained in step (1) to the fermentation medium with an inoculum of 2.0%, and keep the temperature at 28.0°C and pH value 8.6. Cultivate for 22-26 hours under the condition of shaking table rotation speed 200 rpm to obtain fermentation broth. The formula of the fermentation medium is as follows: each 100mL fermentation medium contains 0.85g of glucose, 0.2...
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