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Photorhabdus luminescens Hb1029 and application thereof

A luminescent bacteria, fermentation broth technology, applied in the application, bacteria, fungicides and other directions, can solve the problems of pesticide residues, environmental pollution, food poisoning, etc., and achieve the effects of low cost, wide control spectrum, and reduced use

Active Publication Date: 2018-04-17
POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI
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  • Abstract
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  • Claims
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Problems solved by technology

[0007] An object of the present invention is to provide a high-efficiency, broad-spectrum, and stable-effect photobacterium neophotobacterium in view of the problems of pesticide residues, food poisoning, and environmental pollution caused by the large-scale use of chemical agents in the prevention and control of agricultural diseases and insect pests and post-harvest fruit preservation.

Method used

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  • Photorhabdus luminescens Hb1029 and application thereof
  • Photorhabdus luminescens Hb1029 and application thereof
  • Photorhabdus luminescens Hb1029 and application thereof

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Embodiment Construction

[0029] Isolation of Photobacterium Hb1029

[0030] Isolation of Photobacteria by NBTA, an obligate medium for commensal bacteria of entomopathogenic nematodes. Three healthy larvae of S. mellonella were selected, placed in a petri dish covered with sterilized filter paper, and 500 μL of entomopathogenic nematodes (concentration of 500-600 larvae / mL) were sucked and dropped on the body surface of S. mellonella, and cultured overnight at 25°C. Disinfect the surface of the Mellonella mellonella larvae infected by entomopathogenic nematodes with 75% alcohol cotton, cut off the forefeet, take the body fluid (10-20 μL) into a centrifuge tube containing 200 μL sterile water, mix well, and inoculate On NBTA medium (beef extract 5.0g, peptone 10.0g, sodium chloride 5.0g, agar 20.0g, musk bromothymol blue 0.025g, triphenyltetrazolium chloride 0.040g, water 1L) plate, After coating evenly, incubate at 28°C for 48 hours at a constant temperature, and the blue single colony that appears o...

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Abstract

The invention discloses photorhabdus luminescens Hb1029 and application thereof. Experiments prove that the photorhabdus luminescens Hb1029 shows a good inhibitory effect against various plant bacterial pathogens such as banana fusarium wilt, tomato fusarium wilt, guava shoot blight, pitaya fruit rot as well as multiple plant fungi and citrus canker; furthermore, the photorhabdus luminescens Hb1029 has an activity of inhibiting growth and development of or killing plant pests such as beet armyworm, citrus fruit fly, and citrus leaf miner. The biocontrol bacterium has insecticidal and bacteriostatic effects and a broader spectrum of prevention and control. After being subjected to subculture for a plurality of times, the strain has stable insecticidal and antibacterial effects. The photorhabdus luminescens Hb1029 has a good preservation effect when being used for fresh fruit preservation, and has very good safety at the same time, thus having an important economic significance.

Description

technical field [0001] The invention belongs to the technical field of biopesticides, in particular to a new bacterial strain and its application, in particular to a photorhabdus luminescens Hb1029 and its application. Background technique [0002] At present, the prevention and control of plant diseases and insect pests and the post-harvest preservation of fruits mainly rely on chemical pesticides. However, the extensive use of chemical pesticides will not only cause excessive pesticide residues and resistance of diseases and insect pests, but also pollute the environment and cause problems such as human and animal poisoning. Due to its low side effects, environmental friendliness, and safety to humans and animals, biopesticides are green pesticides with broad prospects and have become an important choice in biological pest control measures. [0003] Studies have shown that entomopathogenic nematodes are safe for humans and animals because they are non-toxic and non-polluti...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00A01P7/04A23B7/155C12R1/01
CPCA23B7/155A23V2002/00A01N63/10C12N1/20C12N1/205C12R2001/01A23V2200/10
Inventor 赖多邵雪花匡石滋凡超田世尧庄镇瑞
Owner POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI
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