The invention relates to a method for quickly modifying
avidin on an interface based on lipidosome. The method comprises the steps of mixing
phospholipid and biotinylated phosphatidyl
ethanol, adding
chloroform for ultrasonic stirring,
drying under
nitrogen, and
vacuum drying; adding a neutral solution, matching films with different bore diameters for a squeezer, and squeezing for multiple times so as to form lipidosomes with different sizes; after cleaning a
glass slide, soaking in a
hydrofluoric acid solution with the concentration being 1 percent for dozens of seconds, then washing with pure water, and
drying under the
nitrogen; putting the
glass slide into a clean utensil, dropwise adding a certain amount of synthetic lipidosome, incubating for several minutes at the temperature higher than the phase-
transition temperature, and using super-pure water for cleaning excessive lipidosome; later, adding the
avidin for incubating for several minutes, using the super-pure water or solution to clean excessive
avidin, and obtaining a glass interface with the avidin modified on the surface. The method is simple, convenient and quick in modification steps, uniform in modification, and complete in sealing, and since
silanization reagents are not introduced during a modification process, the background interference is less. The prepared interface can meet the requirement on single molecule enzymology research.