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Oxygen-carrying phospholipid micro-capsule, preparation method thereof and application of oxygen-carrying phospholipid micro-capsule in preparation of anti-hypoxic drugs

A technology of oxygen-loaded phospholipids and myristoyl phosphatidylcholine, which is applied in the field of medicine, can solve the problems of restricting the marketization process of products, the inability to realize small laboratory tests, and low bioavailability, and achieve a simple, controllable and stable preparation method Good stability and biocompatibility, and uniform particle size

Pending Publication Date: 2022-04-08
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the drug carriers used to carry oxygen in the existing reports have relatively low bioavailability, and it is impossible to realize the transfer from small laboratory test to pilot scale production, which seriously restricts the marketization process of related products

Method used

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  • Oxygen-carrying phospholipid micro-capsule, preparation method thereof and application of oxygen-carrying phospholipid micro-capsule in preparation of anti-hypoxic drugs
  • Oxygen-carrying phospholipid micro-capsule, preparation method thereof and application of oxygen-carrying phospholipid micro-capsule in preparation of anti-hypoxic drugs
  • Oxygen-carrying phospholipid micro-capsule, preparation method thereof and application of oxygen-carrying phospholipid micro-capsule in preparation of anti-hypoxic drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A preparation method of oxygen-loaded phospholipid microcapsules, comprising the steps of:

[0040] Add 20 mg of myristoylphosphatidylcholine, 75 mg of dipalmitoylphosphatidylcholine, 5 mg of dipalmitoylphosphatidylethanol-polyethylene glycol 2000-triphenylphosphate, and 10 mL of dimethyl sulfoxide to a vessel using phacoemulsification Machine, ultrasonic power is 300W, ultrasonic time is 10min, dissolves, and obtains clear solution; Evaporate under reduced pressure to remove dimethyl sulfoxide, a layer of uniform translucent pale yellow film is attached on the inner wall of the container; Vacuum dry, add 5mL ultrapure water and Hydrate in an ice bath. The set temperature for hydration in the ice bath is 0°C, and the hydration time is 10 minutes to obtain phospholipid microcapsules, which are placed in an ice bath; 3 mL of perfluorohexane is added to obtain a mixed system. 100W, 5min / time, 10 times, each interval of 5min, ultrasonically dispersed until the mixed system ...

Embodiment 2

[0043] A preparation method of oxygen-loaded phospholipid microcapsules, comprising the steps of:

[0044] Add 20mg myristoylphosphatidylcholine, 65mg dipalmitoylphosphatidylcholine, 8mg dipalmitoylphosphatidylethanol-polyethylene glycol 2000-triphenyl phosphate and a mixture of 10mL chloroform and methanol ( Organic solvent: the volume ratio of chloroform to methanol is 4:1), using a ultrasonic emulsification machine, the ultrasonic power is 200W, and the ultrasonic time is 15min, dissolve to obtain a clear solution; remove the organic solvent by evaporation under reduced pressure, and adhere to the inner wall of the container A uniform and transparent light yellow film; vacuum drying, adding 5mL ultrapure water and hydrating in an ice bath, the set temperature for hydration in the ice bath is 0°C, and the hydration time is 15min to obtain phospholipid microcapsules, which are placed in In an ice bath; add 8mL perfluorohexane to obtain a mixed system. At a power of 80W, 5min / ...

Embodiment 3

[0046]A preparation method of oxygen-loaded phospholipid microcapsules, comprising the steps of:

[0047] Add 20 mg of myristoylphosphatidylcholine, 85 mg of dipalmitoylphosphatidylcholine, 2 mg of dipalmitoylphosphatidylethanol-polyethylene glycol 2000-triphenyl phosphate and a mixture of 10 mL of chloroform and methanol ( Organic solvent, the volume ratio of chloroform and methanol is 4:1), use ultrasonic emulsification machine, ultrasonic power is 350W, ultrasonic time is 5min, dissolve, obtain clear solution; A uniform and transparent light yellow film; vacuum drying, adding 5mL ultrapure water and hydrating in an ice bath, the set temperature for hydration in the ice bath is 0 °C, and the hydration time is 5min to obtain phospholipid microcapsules, which are placed in In an ice bath; add 12mL of perfluorohexane to obtain a mixed system, and at a power of 120W, 5min / time, 5 times, with an interval of 5min, ultrasonically disperse until the mixed system has no stratificatio...

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Abstract

The invention discloses an oxygen-carrying phospholipid microcapsule, a preparation method thereof and application of the oxygen-carrying phospholipid microcapsule in preparation of an anti-hypoxia drug, and the preparation method comprises the following steps: adding myristoyl phosphatidylcholine, dipalmitoyl phosphatidylcholine, dipalmitoyl phosphatidylethanole-polyethylene glycol 2000-triphenyl phosphate and an organic solvent into a container, and carrying out ultrasonic treatment to obtain a clear solution; performing reduced pressure evaporation to remove the organic solvent, and adhering a layer of uniform and transparent faint yellow film on the inner wall of the container; carrying out vacuum drying, adding ultrapure water, hydrating in an ice bath to obtain phospholipid microcapsules, and putting the phospholipid microcapsules in the ice bath; adding perfluorohexane to obtain a mixed system, and performing ultrasonic dispersion until the mixed system is not layered to obtain an emulsion; and adding a freeze-drying protective agent, freeze-drying, storing at 4 DEG C, and redissolving and oxygenating before use to obtain the oxygen-carrying phospholipid microcapsule. The preparation method is simple and controllable, and raw materials and auxiliary materials are easy to obtain; the prepared oxygen-carrying phospholipid micro-capsule is uniform in particle size and good in stability and biocompatibility, oxygen can be supplemented to an organism in time, and the effect of treating diseases is achieved.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to an oxygen-carrying phospholipid microcapsule, a preparation method and an application in preparation of anti-hypoxic drugs. Background technique [0002] A series of diseases will appear when the body is deprived of oxygen for too long. Among them, lung diseases are the main ones, such as lung expansion, lung tissue fibrosis, calcification, etc. leading to bronchial asthma, and even hypercapnia and hypoxemia in severe cases, leading to different degrees of respiratory failure and death. With the development of society, more and more people choose to travel to plateau areas. Due to the high altitude, thin air, and low temperature in plateau areas, the relevant personnel will have different degrees of altitude sickness. Especially when entering the plateau quickly from the plain, it is easy to cause high altitude pulmonary edema, high altitude cerebral edema and other diseases that seriously...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/50A61K47/24A61K33/00A61P11/00
Inventor 李楠周岳梁晓阳
Owner TIANJIN UNIV
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