32 results about "PARAQUAT POISONING" patented technology

The invention relates to a paraquat-containing paste agent and a preparation method thereof. The paraquat paste agent is prepared by thoroughly mixing an effective component of 10% to 50% by mass of paraquat dichloride and a proper quantity of water-soluble high polymers, a surfactant, an emetic, a filler, a moisturizing agent, a coloring agent and an olfactory warning agent. At present, the dosage form of paraquat is mainly water agent, being easily administered orally. According to the invention,the paste agent prepared from paraquat, which is not easily administered, can effectively reduce the risk of paraquat poisoning, and compared with other types of conventional preparations, the paraquat paste agent has a good environmental compatibility and an excellent weed control effect.

The invention relates to a kit for rapid determination of paraquat concentration in blood. The kit mainly comprises a reagent I for removing proteins, a reagent II for removing nucleic acid and preventing interference, a kit solvent, a paraquat standard and a paraquat calibrator. The invention also relates to an application method of the kit. The kit can rapidly determine the paraquat concentration in the blood of a patient, and provides rapid, simple, accurate, reliable and effective reference for clinical diagnosis of paraquat intoxication.

The invention relates to a reagent for qualitatively detecting paraquat and a method for detecting the paraquat in urine, which are used for quickly detecting the most common pesticide poisoning, namely paraquat poisoning. The reagent comprises a reagent A and a reagent B, wherein the reagent A is sodium bicarbonate; and the reagent B is alkali sodium dithionite or sodium dithionite. The method for detecting the paraquat comprises the following steps of: taking 10ml of urine of a patient, putting the urine in a clean plastic cup, adding 2.0g of the reagent A into the cup, and after the reagent A is completely and uniformly dissolved, adding 1.0g of the reagent B into the cup, wherein if the urine contains paraquat ions, the urine immediately turns blue, and the darker the blue color is, the higher the concentration of the paraquat in the urine is. The reagent has the advantages of high accuracy, no false positive and false negative, high sensitivity, specificity and uniqueness.

The invention relates to a method for rapidly and quantitatively detecting concentration of paraquat in patient's blood. A kit used in the detection method is composed of a deproteinization I, a reagent II, a solvent III and a paraquat standard substance IV. The invention also provides a ratio of all above reagents. An optimum volume ratio of the deproteinization I acetonitrile to the reagent II 20% trichloroacetic acid to serum is 1:1.3:6, that is 100[mu]L of acetonitrile and 130[mu]L of the 20% trichloroacetic acid solution are added to 600[mu]L of a sample serum to realize a best deproteinization effect, the deproteinized sample serum is centrifuged, the centrifuged sample serum is directly detected by using an ultraviolet spectrophotometer, and the accurate concentration of paraquat in the sample serum is obtained according to a calculation equation obtained through a standard substance curve. The method allows the concentration of paraquat in patient's blood to be rapidly and quantitatively detected, and provides most fast and effective bases for clinic treatment of paraquat poisoning.

The invention relates to treatment of lung injury by using mesenchymal stem cells, and particularly relates to treatment of paraquat poisoning lung injury by using umbilical cord mesenchymal stem cells.

The objective of the invention is to provide a Chinese herbal compound used for treating paraquat poisoning. According to the invention, Chinese traditional treatment theories of invigoration of blood, removal of stasis, elimination of necrotic tissue, engendering of flesh, nourishment to the lung, promotion of blood circulation, protection of the liver and the kidney and the like are used as support, and by nourishing and invigorating blood, human immunity is enhanced, self-repairing capacity and transporting and digesting functions of visceral organs are improved and formation of pulmonary fibrosis is inhibited. The Chinese herbal compound comprises gecko, Chinese caterpillar fungus, dysosma pleiantha, tendril-leaved fritillary bulb, Artemisia capillaris, rhizome anemones altaicae, cassia twig, white peony root, vegetable sponge of luffa, Eclipta alba, dark plum, ginseng, donkey-hide gelatin and furnace soil.

The invention discloses an application of glycosyl modified polyphenol compounds as drugs for relieving paraquat poisoning and treating pulmonary fibrosis. The glycosyl modified polyphenol compounds have the structure shown in the description. The glycosyl modified polyphenol compounds have a good oxidation resistance function and a lung protection function and have a notable protection function on lung injury caused by paraquat, and a mechanism is related with reduction of formation of oxygen radicals, relieving of oxidative stress and inflammatory reaction, increase of content of GSH in blood serum and SOD in lung tissue and reduction of content of MDA and HYP in lung tissue. The glycosyl modified polyphenol compounds can effectively inhibit bleomycin-induced pulmonary fibrosis of mice,and the mechanism is related with reduction of expression of TGF-beta 1, IL-6, alpha-SMA, P-smad2, type I collagen and type III collagen in lung tissue, increase of content of GSH and SOD in blood serum and reduction of the content of HYP in lung tissue.

The invention provides a new use of sulfonatocarlix(5)arene in preparing a medicine for treating paraquat poisoning. The sulfonatocarlix(5)arene reduces probability of contacts between paraguat and NAD(P)H in vivo by inclusion coordination of the sulfonatocarlix(5)arene and the paraguat, and reduces oxidizing ability of the paraguat, thus inhibiting generation of a free radical (HO<.>) which is poisonous to cells in vivo. Additionally, the lower edge of the sulfonatocarlix(5)arene is rich in phenolic hydroxyl structures which can provide active hydrogen to inactivate the HO<.> free radical. Experiments on mice prove that the sulfonatocarlix(5)arene is nontoxic or low toxic and greatly reduces toxicity of the paraguat. After a mouse is poisoned by orally taking the paraguat (dose: 106.99mg / kg), good curative effect can be achieved by orally taking two-fold sulfonatocarlix(5)arene for once, the total lethality is decreased from 90% to 30%, and the lowest lethality is even decreased to 0%. After a mouse is poisoned, good effect can be achieved by orally taking the sulfonatocarlix(5)arene in two hours after poisoning.

The invention discloses a paraquat detection reagent tube. The reagent tube comprises a plastic liquid sucking tube (1); a colorless capillary tube (2) of which the two ends are sealed in a melting manner and the interior is filled with sodium bicarbonate, and a brown capillary tube (3) of which the two ends are sealed in a melting manner and the interior is filled with sodium hydrosulfite are arranged in the plastic liquid sucking tube; the plastic liquid sucking tube is movably connected with a plastic liquid sucking tube cap (4). The reagent tube is simple to operate, and long in available period; a reaction reagent is free from failure in one year; the reagent tube is high in sensitivity, can be semiquantitative when in colorimetric combination with a colorimetric board, and be quantitative accurately when combining with an ultraviolet light splitting luminosity instrument, and can be applied to a noncausal suspected paraquat poisoning patient to achieve the goal of clarifying a diagnosis at the earliest.

The invention discloses a method for treating acute poisoning of canine paraquat. The main component of a medicine used in the method is the hydroquinone compound or pharmaceutically-acceptable salt thereof, preferentially 9,10-anthrahydroquinone-2,6-disulfonic acid or 9,10-anthrahydroquinone-2,6-disulfonate (referred to as AHQDS). The application method includes the steps that according to the paraquat poisoning dosage in cooperation with different doses of detoxification medicines, treatment is conducted through a gavage mode. The medicine can obviously reduce the toxic reaction of canine paraquat, prevents the lung injuries caused by paraquat, and greatly increases the survival rate of paraquat acutely poisoned canines.

The invention discloses a method for rapidly detecting paraquat in a biological matrix sample, which uses a real-time direct analysis ion source and a mass spectrometer for detection, does not treat the detected biological matrix sample, and is an in-situ detection method. Pretreatment of traditional means is omitted, and rapid and real-time detection of biological matrix samples poisoned by paraquat is achieved.

The invention discloses an oral liquid for treating paraquat poisoning and a preparation method of the oral liquid, and belongs to the field of traditional Chinese medicine. The active ingredient of the oral liquid consists of the following raw materials: exocarpium, peppermint, red fungus, fraxinus malacophylla, ginkgo, rosa bracteata root, betel nut, alocasia cucullata, drymoglossum piloselloides presl, jasminum laurifolium, japanese balanophora, chrysanthemum morifolium, humata tyermanni, black pepper, fiveleaf akebia fruit and michelia figo. The selected medicinal materials of the oral liquid are appropriately matched in accordance with the theories of traditional Chinese medicine and pharmacy as well as modern medicine and pharmacy, and the oral liquid has functions of regulating qi and tonifying spleen, clearing damp and promoting diuresis, relieving swelling and expelling toxin, and protecting liver and kidney; the oral liquid has a significant curative effect on treating paraquat poisoning, and the oral liquid can be used for enhancing body resistance and improving the self-repairing capability of visceral organs; the oral liquid is short in treatment course, capable of taking effect, safe and reliable, free from any toxic and side effects, low in cost, capable of achieving a purpose of treating both symptoms and root causes and is worthy of wide popularization and application in clinical.

The invention discloses an application of Xuebijing in preparing medicament for treating paraquat poisoning, which is used for treating pulmonary fibrosis caused by paraquat poisoning. Xuebijing injection is used for carrying out early interference therapy on paraquat poisoned patients so that the TGF-beta 1 and 111 type PIIIP level in the blood serum of the paraquat poisoned patients can be obviously lowered, thus restraining the occurrence and development of pulmonary fibrosis caused by paraquat poisoning. Interference therapy of use of Xuebijing injection on the paraquat poisoned patients can obviously improve the respiratory function of the patients, relieve the physiological damage of the lung tissue after paraquat poisoning, reduce the occurrence rate of pulmonary fibrosis, lessen the pulmonary fibrosis degree and lower the fatality rate of the paraquat poisoned patients, thus improving the prognosis of the patients.

A preparation for treating oral mucositis caused by paraquat. The preparation particularly includes following components: 250ml of a 0.9% sodium chloride solution, 0.16g of 2% lidocaine, 0.005g of dexamethasone and 0.08g of gentamicin. The preparation can reduce oral mucositis damage of a paraquat poisoning patient, can alleviating pain, can resist infection and can inhibit immunity. The preparation is used in a manner of mouth wash. A patient uses the preparation in the mouth-washing manner per 2 hours in daytime so that stimulation during local drug delivery is avoided and pain is alleviated. By means of frequent mouth washing, oral cavity can be moistened and large residue blocks and necrotic tissues can be removed. The preparation can reduce secretion and dental plaque, promotes a self-cleaning function of the oral cavity. A mouth washing liquid is low in cost and has an important clinical application value.

The invention provides a toxicant qualitative and semi-quantitative detection kit and a toxicant qualitative and semi-quantitative method. A 5ml detection liquid centrifugal tube, a 5ml blank centrifugal tube, a reaction colourimetric card and a qualitative detection strip are arranged in the kit; the 5ml detection liquid centrifugal tube contains 3ml detection liquid; and the reaction colourimetric card is that detection liquid is added when in different paraquat concentrations. The toxicant qualitative method comprises the steps of: (1), adding a sample to be detected into the detection liquid, wherein the mass ratio of the sample to be detected to the detection liquid is 1:4 to 4:1; (2), if the colour of the mixed solution in the step (1) is changed, representing that the sample to be detected contains paraquat; and (3), when immersing the immersing end of the qualitative detection strip in the sample to be detected, instantly changing the colour in a reaction tank, and representingthat the sample to be detected contains paraquat. According to the toxicant qualitative and semi-quantitative detection kit and the toxicant qualitative and semi-quantitative method in the invention,by utilization of the principle that sodium hydrosulfite and paraquat perform colour changing reaction in an alkaline condition, the qualitative detection strip and the qualitative and semi-quantitative detection kit are prepared; a novel diagnosis method can be provided for clinically excluding paraquat poisoning; and the kit effectively serves in clinic, and has very high social value.

The invention discloses a pharmaceutical composition containing moxifloxacin for resisting pulmonary fibrosis. The pharmaceutical composition comprises active components of moxifloxacin or medicinal salt thereof and curcumin. Moxifloxacin and curcumin in combination have the cooperative effect of treating paraquat poisoning-induced acute lung injury and resisting pulmonary fibrosis; the amount of moxifloxacin is only one fourth of the conventional dosage. Therefore, the composition has low cost, little side effects and low adverse reaction rate, and solves the technical problem of strong drug side effect.

The invention discloses an application of glycosyl modified polyphenol compounds as drugs for relieving paraquat poisoning and treating pulmonary fibrosis. The glycosyl modified polyphenol compounds have the structure shown in the description. The glycosyl modified polyphenol compounds have a good oxidation resistance function and a lung protection function and have a notable protection function on lung injury caused by paraquat, and a mechanism is related with reduction of formation of oxygen radicals, relieving of oxidative stress and inflammatory reaction, increase of content of GSH in blood serum and SOD in lung tissue and reduction of content of MDA and HYP in lung tissue. The glycosyl modified polyphenol compounds can effectively inhibit bleomycin-induced pulmonary fibrosis of mice,and the mechanism is related with reduction of expression of TGF-beta 1, IL-6, alpha-SMA, P-smad2, type I collagen and type III collagen in lung tissue, increase of content of GSH and SOD in blood serum and reduction of the content of HYP in lung tissue.

The invention discloses application of lysine to preparation of a medicine for inhibiting or treating paraquat poisoning. The lysine can obviously improve the reduction of the weight, diet and drinking water of the mouse caused by paraquat poisoning, improve the pulmonary fibrosis and death of the mouse after the mouse is poisoned, and can be used for treating the paraquat poisoning and relieving the progress of the in-vivo pulmonary fibrosis after the paraquat poisoning.

The invention provides a new use of sulfonatocarlix(5)arene in preparing a medicine for treating paraquat poisoning. The sulfonatocarlix(5)arene reduces probability of contacts between paraguat and NAD(P)H in vivo by inclusion coordination of the sulfonatocarlix(5)arene and the paraguat, and reduces oxidizing ability of the paraguat, thus inhibiting generation of a free radical (HO<.>) which is poisonous to cells in vivo. Additionally, the lower edge of the sulfonatocarlix(5)arene is rich in phenolic hydroxyl structures which can provide active hydrogen to inactivate the HO<.> free radical. Experiments on mice prove that the sulfonatocarlix(5)arene is nontoxic or low toxic and greatly reduces toxicity of the paraguat. After a mouse is poisoned by orally taking the paraguat (dose: 106.99mg / kg), good curative effect can be achieved by orally taking two-fold sulfonatocarlix(5)arene for once, the total lethality is decreased from 90% to 30%, and the lowest lethality is even decreased to 0%. After a mouse is poisoned, good effect can be achieved by orally taking the sulfonatocarlix(5)arene in two hours after poisoning.

The patent of invention relates to a method for detecting the paraquat in urine, which are used for quickly detecting the most common pesticide poisoning, namely paraquat poisoning. The reagent comprises a reagent A and a reagent B, wherein the reagent A is sodium bicarbonate; and the reagent B is alkali sodium dithionite or sodium dithionite. The method for detecting the paraquat comprises the following steps of: taking 10ml of urine of a patient, putting the urine in a clean plastic cup, adding 2.0g of the reagent A into the cup, and after the reagent A is completely and uniformly dissolved, adding 1.0g of the reagent B into the cup, wherein if the urine contains paraquat ions, the urine immediately turns blue, and the darker the blue color is, the higher the concentration of the paraquat in the urine is. The reagent has the advantages of high accuracy, no false positive and false negative, high sensitivity, specificity and uniqueness.

The invention relates to pharmaceutical composition for treating paraquat poisoning. The composition is prepared from the following components in parts by weight: 7-17 parts of mint, 7-17 parts of geckoes, 1-11 parts of iresine herbstii, 1-11 parts of caterpillar fungi, 4-14 parts of solomonseal rhizomes, 7-17 parts of largeleaf gentian roots, 7-17 parts of virgate wormwood herbs, 1-11 parts of sweet wormwood herbs, 11-21 parts of poria cocos, 5-15 parts of common monkshood daughter roots, 1-11 parts of Cape jasmine fruits, 1-11 parts of licorice roots and 5-15 parts of macartney rose roots. The drugs in the formula are compatible, monarch and minister drugs are synergetic, and adjuvant drugs and envoy drugs assist together. The pharmaceutical composition is aimed at enhancing the human immunity, improving the self-repair capacity as well as the transformation and transport function of viscera and resisting pulmonary fibrosis and peripheral viscera block finally.

The invention provides a human lung cancer paraquat-resistant cell strain. A human lung cancer cell strain A549 is used as an induction object; an in-vitro induction method of periodic exposure and progressive increase of paraquat concentration is adopted; and a paraquat-resistant cell strain A549 / PQ is built. The cell strain is collected in CGMCC (China General Microbiological Culture Collection Center) on June 16, 2015; and the collection number is CGMCC NO.10892. The cell strain has the typical paraquat-resistant characteristic; by using the biological characteristic, a study model can be provided for further studying a paraquat toxicity reversion path, extracting a paraquat poisoning evaluation marker, exploring a novel anti-oxidization path and the like; and high scientific research and production and application values are realized.

A human lung cancer cell strain A549 is adopted as an inducing object, an in-vitro inducing method for performing periodic exposure and gradually increasing the concentration of paraquat is adopted, and a paraquat-resistant cell strain A549 / PQ is established. The cell strain is preserved in China General Microbiological Culture Collection Center at June 16th 2015, and the preservation number is CGMCC NO.10892. The cell strain has the typical paraquat-resistant characteristic, and the biological characteristic provides a research model for further performing research on reversing of toxicity of paraquat, extracting an evaluation marker of paraquat poisoning and exploring a new anti-oxidant way, and has high scientific research value and production and application value.

The invention relates to the technical field of new application of medicines, and discloses new application of niclosamide. The invention specifically discloses application of niclosamide in preparation of a medicine for treating bipyridine herbicide, especially paraquat poisoning, application in preparation of a medicine for treating organ injury caused by poisoning, and a herbicide comprising paraquat and niclosamide.

The invention relates to the field of medicines, and discloses a traditional Chinese medicinal composition for treating paraquat poisoning. The traditional Chinese medicinal composition is prepared from the following raw materials in parts by weight: 5-8 parts of toad, 0.5-0.8 part of musk, and 3-4 parts of bezoar. The invention further discloses a preparation method of the traditional Chinese medicinal composition. The traditional Chinese medicinal composition is high in cure rate when used for treating paraquat poisoning, and thus having a good clinical application prospect.

The invention provides a human lung cancer paraquat-resistant cell strain. A human lung cancer cell strain A549 is used as an induction object; an in-vitro induction method of periodic exposure and progressive increase of paraquat concentration is adopted; and a paraquat-resistant cell strain A549 / PQ is built. The cell strain is collected in CGMCC (China General Microbiological Culture Collection Center) on June 16, 2015; and the collection number is CGMCC NO.10892. The cell strain has the typical paraquat-resistant characteristic; by using the biological characteristic, a study model can be provided for further studying a paraquat toxicity reversion path, extracting a paraquat poisoning evaluation marker, exploring a novel anti-oxidization path and the like; and high scientific research and production and application values are realized.

The invention, which relates to the technical field of paraquat detoxification, discloses application of cucurbituril [7] in paraquat detoxification. The research result shows that the cucurbituril [7] can form a compound with paraquat in the gastrointestinal tract to reduce the intestinal absorption of paraquat, thereby realizing a detoxification effect. The cucurbituril [7] can be used in fieldsof preparation of special-effect oral antidotes for treating paraquat poisoning and the like and is used for paraquat detoxification.

The invention belongs to the field of emergency medicine and particularly relates to an application of methylene blue to the treatment of early acute paraquat poisoning. The methylene blue is used for treating a pulmonary fibrosis disease caused by the paraquat poisoning. When the methylene blue is adopted for the early intervening treatment of a paraquat poisoning patient, the lung tissue superoxide dismutase (SOD) activity of the paraquat poisoning patient can be obviously increased, and the malonaldehyde (MDA) content and the lactic dehydrogenase (LDH) activity can be reduced, so that the pathological damage of lung tissues after paraquat poisoning is reduced, the occurrence and the development of paraquat poisoning pulmonary fibrosis are restrained, and the mortality of the paraquat poisoning patient is reduced, therefore, the patient prognosis is improved.

The invention relates to a kit for rapid determination of paraquat concentration in blood. The kit mainly comprises a reagent I for removing proteins, a reagent II for removing nucleic acid and preventing interference, a kit solvent, a paraquat standard and a paraquat calibrator. The invention also relates to an application method of the kit. The kit can rapidly determine the paraquat concentration in the blood of a patient, and provides rapid, simple, accurate, reliable and effective reference for clinical diagnosis of paraquat intoxication.

A human lung cancer cell strain A549 is adopted as an inducing object, an in-vitro inducing method for performing periodic exposure and gradually increasing the concentration of paraquat is adopted, and a paraquat-resistant cell strain A549 / PQ is established. The cell strain is preserved in China General Microbiological Culture Collection Center at June 16th 2015, and the preservation number is CGMCC NO.10892. The cell strain has the typical paraquat-resistant characteristic, and the biological characteristic provides a research model for further performing research on reversing of toxicity of paraquat, extracting an evaluation marker of paraquat poisoning and exploring a new anti-oxidant way, and has high scientific research value and production and application value.