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Application of cucurbituril [7] in paraquat detoxification.

A paraquat and melon ring technology, applied in the application field of melon ring[7] in paraquat detoxification, can solve the problems of no good effect, no PQ detoxification special effect method, etc., to inhibit the increase of cellular reactive oxygen species level. , the effect of reducing the increase in the level of inflammatory factors and prolonging the survival time

Pending Publication Date: 2020-03-06
UNIVERSITY OF MACAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unfortunately, such treatment measures do not have a good clinical effect, and so far there is no specific method for PQ detoxification

Method used

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  • Application of cucurbituril [7] in paraquat detoxification.
  • Application of cucurbituril [7] in paraquat detoxification.
  • Application of cucurbituril [7] in paraquat detoxification.

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Synthesis of Cucurbit[7]

[0078] 100g of glucuronide was thoroughly mixed with 42.2g of paraformaldehyde powder. While vigorously stirring, 142 mL of concentrated hydrochloric acid was added dropwise. After standing for 30 minutes, heat to 100 degrees and keep for 20 hours. After the suspension was cooled to room temperature, the cucurbitanyl ring [7] (abbreviated as CB [7] hereinafter) was isolated using the method reported in the literature (J. Org. Chem. 66, 8094-8100 (2001)).

Embodiment 2

[0080] CB[7] combined with PQ model

[0081] 1 method:

[0082] Use ChemBioOffice Ultra 14.0 software to draw the three-dimensional structures of CB[7] and PQ, and use AutoDockTools to generate pdb files. The combination process of CB[7] and PQ is simulated by AutoDock Vina.

[0083] The lattice calculation range is set to The lattice calculation unit is The search space center is set to and (x, y, z). The number of GA runs is set to 100, and other parameters are the default values ​​of AutoDock Vina. Conformation searches were performed using the Lamarckian genetic algorithm. The energy-minimized complex structure was optimized with ChemBioOffice Ultra 14.0.

[0084] 2 results:

[0085] The combination of PQ and CB[7] was simulated by AutoDock Vina, and the complex structure with the lowest energy is shown in figure 2 . figure 2 The left side of A shows the structure of the melon ring [7], and the right side shows the structure of PQ. figure 2 B shows the C...

Embodiment 3

[0091] In Vitro Experiment of Detoxification Effect of CB[7] on PQ

[0092] Example 2 proves that CB[7] can effectively bind PQ under different pH environments. In this example, we evaluated the detoxification effect of CB[7] on PQ through in vitro cell experiments. We used a macrophage cell line RAW264.7 commonly used in in vitro cytotoxicity evaluation to study the protective effect of CB[7] (molar ratio 1:1) at different PQ concentrations.

[0093] 1 method is as follows:

[0094] 1.1 Determination of cell viability

[0095] Twelve hours after the cells were seeded in 96-well plates, different concentrations of PQ with or without CB[7] were added. The cell viability at the set time was determined by the CCK-8 method.

[0096] 1.2 Apoptosis assay

[0097] Will 2×10 5 RAW264.7 cells were suspended in 1 mL medium and planted in 12-well plates. Various concentrations of PQ with or without CB[7] were added for incubation. Cells were collected at set time points, stained w...

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Abstract

The invention, which relates to the technical field of paraquat detoxification, discloses application of cucurbituril [7] in paraquat detoxification. The research result shows that the cucurbituril [7] can form a compound with paraquat in the gastrointestinal tract to reduce the intestinal absorption of paraquat, thereby realizing a detoxification effect. The cucurbituril [7] can be used in fieldsof preparation of special-effect oral antidotes for treating paraquat poisoning and the like and is used for paraquat detoxification.

Description

technical field [0001] The present invention relates to the technical field of detoxification of paraquat, in particular, relates to the application of cucurbit [7] in detoxification of paraquat. Background technique [0002] Paraquat, chemical name is 1,1'-dimethyl-4,4'-bipyridine cationic salt dichloride, chemical formula: C 12 h 14 Cl 2 N 2 , is a fast-killing herbicide with contact and certain systemic effects. It can be quickly absorbed by the green tissue of plants, causing them to die. Has no effect on non-green organizations. In the soil, it quickly combines with the soil to passivate, and is ineffective for plant roots, perennial rhizomes and perennial roots. [0003] Paraquat (PQ) is the most widely used pesticide in the world. Accidental or voluntary ingestion of PQ can quickly lead to multiple organ failure, with a mortality rate of 60%. In clinical practice, activated charcoal gastric lavage is often used for the early treatment of PQ, but the actual resu...

Claims

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Application Information

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IPC IPC(8): A61K31/4188A61P39/02
CPCA61K31/4188A61P39/02
Inventor 王瑞兵张相军李升可
Owner UNIVERSITY OF MACAU
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