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Treatment effect of umbilical cord mesenchymal stem cells on paraquat poisoning lung injury

A technology of mesenchymal stem cells and paraquat is applied in the field of treatment of lung injury caused by paraquat poisoning by umbilical cord mesenchymal stem cells. The effect of reducing the quality of life and animal mortality

Inactive Publication Date: 2014-05-28
AFFILIATED HOSPITAL CHINA ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Due to the small lethal dose of poisoning, no specific antidote, and the poor effect of conventional symptomatic treatment, the mortality rate remains high
However, there is no research on the use of MSCs in the treatment of paraquat poisoning-induced pulmonary fibrosis

Method used

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  • Treatment effect of umbilical cord mesenchymal stem cells on paraquat poisoning lung injury
  • Treatment effect of umbilical cord mesenchymal stem cells on paraquat poisoning lung injury
  • Treatment effect of umbilical cord mesenchymal stem cells on paraquat poisoning lung injury

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Experimental program
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Embodiment

[0023] Materials and Methods

[0024] 1. Animals

[0025] Healthy adult female C57BL / 6 mice, weighing 19-21g, moved in a barrier environment with constant temperature and humidity indoors, artificial lighting for 12h / d, free access to food and water, and adaptive feeding for 1 week before the experiment.

[0026] 2. Isolation, culture and identification of UCMSC

[0027] UCMSC took healthy fetuses from the Air Force General Hospital, signed informed consent, and had no infectious diseases. The umbilical cord sample was rinsed and cut into 2-3mm 3 The small pieces were digested with 1% collagenase digestion solution at 37°C for 2 h, filtered through a 70 μm sieve, the supernatant was collected, and the cells were collected by centrifugation. After washing with PBS, add 5 mL of culture medium to resuspend the cells, and culture in a 5% CO2 incubator at 37 °C. When 80%-90% confluent, passage at a ratio of 1:2 or 1:3. Cells of passage 6-8 were used for transplantation in anim...

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Abstract

The invention relates to treatment of lung injury by using mesenchymal stem cells, and particularly relates to treatment of paraquat poisoning lung injury by using umbilical cord mesenchymal stem cells.

Description

technical field [0001] The invention relates to the field of mesenchymal stem cell therapy, in particular to the treatment of paraquat poisoning lung injury by umbilical cord mesenchymal stem cells. Background technique [0002] Paraquat (PQ) is a widely used herbicide with strong toxicity to humans. Due to the small lethal dose of poisoning, no specific antidote, and the poor effect of conventional symptomatic treatment, the mortality rate remains high. The characteristic change of paraquat poisoning is lung injury. In the early stage, alveolar epithelial cells are damaged, alveolar hemorrhage and edema, and in the late stage, alveolar and pulmonary interstitial fibrosis is manifested. [0003] In recent years, with the progress of stem cell research, more and more researches have been done on the treatment of clinical refractory and intractable diseases. Mesenchymal stem cells (MSCs) are widely favored due to their advantages such as convenient extraction, strong regener...

Claims

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Application Information

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IPC IPC(8): A61K35/44A61P11/00A61K31/573A61K35/51
Inventor 吕双红王慧娜杜丽欣刘兵
Owner AFFILIATED HOSPITAL CHINA ACADEMY OF MILITARY MEDICAL SCI
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