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Method for rapidly and quantitatively detecting concentration of paraquat in patient's blood

A quantitative detection and paraquat technology, applied in the field of rapid quantitative detection of paraquat concentration in blood, can solve the problems of long time consumption, still existing paraquat chromatographic peak tailing image, affecting the accuracy of detection results, etc. Simplify and realize the effect of experimental conditions

Inactive Publication Date: 2015-11-18
陕西博世康医药科技有限公司
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Problems solved by technology

"Determination of Paraquat in Biological Fluids by High Performance Liquid Chromatography" disclosed on pages 388-389 of Volume 22, Issue 6, 2007 of "China Law Journal" and 160- published on page 161 uses ion-exchange solid-phase extraction to pretreat paraquat plasma samples, which has the disadvantages of complicated and time-consuming sample processing; in addition , although the ion-pairing reagent sodium octane sulfonate was added to the mobile phase to improve the peak shape of paraquat, the tailing image of the paraquat chromatographic peak still exists; using the external standard method, operating errors will affect the detection results Accuracy; the retention time of paraquat is about 12 minutes, exceeding 10 minutes

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  • Method for rapidly and quantitatively detecting concentration of paraquat in patient's blood

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experiment example 1PQ

[0020] Clinical Application of Experimental Example 1 PQ Serum Detection Method

[0021] Patient Li Moumou, female, 15 years old, had a quarrel with her family one day and took about 20mL of herbicide paraquat solution orally. Blood samples were collected from the patients after admission, 1 h after the first hemoperfusion, and 1 h after the second hemoperfusion. 6:1:1.3) and mix well, centrifuge at 12000r / min for 10min, take the supernatant, adjust to zero with distilled water, measure the absorbance with a UV spectrophotometer at a wavelength of 257nm, and substitute it into the equation y=0.0935x+0.2964 to obtain the sample serum The concentrations of paraquat were 10.22, 8.89, 3.56 μg·mL -1 .

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Abstract

The invention relates to a method for rapidly and quantitatively detecting concentration of paraquat in patient's blood. A kit used in the detection method is composed of a deproteinization I, a reagent II, a solvent III and a paraquat standard substance IV. The invention also provides a ratio of all above reagents. An optimum volume ratio of the deproteinization I acetonitrile to the reagent II 20% trichloroacetic acid to serum is 1:1.3:6, that is 100[mu]L of acetonitrile and 130[mu]L of the 20% trichloroacetic acid solution are added to 600[mu]L of a sample serum to realize a best deproteinization effect, the deproteinized sample serum is centrifuged, the centrifuged sample serum is directly detected by using an ultraviolet spectrophotometer, and the accurate concentration of paraquat in the sample serum is obtained according to a calculation equation obtained through a standard substance curve. The method allows the concentration of paraquat in patient's blood to be rapidly and quantitatively detected, and provides most fast and effective bases for clinic treatment of paraquat poisoning.

Description

technical field [0001] The invention relates to a method for detecting pesticides, in particular to a method for rapidly and quantitatively detecting the concentration of paraquat in blood. Background technique [0002] Paraquat (1,1.-Dimethyl-4,4.-dichlorodipyridine, PQ) has become a widely used herbicide in the past 30 years along with PQ, and it has caused many cases of acute and chronic poisoning . The poisoning caused by paraquat has strong toxicity and extensive damage, and there is no specific antidote so far. Clinically, the prognosis of patients is poor, the mortality rate is high, and the clinical symptoms are not proportional to the degree of damage. Therefore, rapid detection of its blood concentration can give doctors a lot of great reference value. [0003] At present, the detection of paraquat concentration mainly includes high performance liquid chromatography, gas chromatography, thin layer chromatography, immunoassay, capillary electrophoresis and so on. ...

Claims

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Application Information

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IPC IPC(8): G01N21/33G01N1/28G01N1/38
Inventor 梁秦
Owner 陕西博世康医药科技有限公司
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