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Kit for rapid determination of paraquat concentration in blood

A technology of paraquat and kit, applied in the field of kits for rapid detection of paraquat concentration in blood, can solve the problems of many detection steps, many influencing factors, and many interference factors, and achieve simple equipment, wide popularization and application wide range of effects

Inactive Publication Date: 2012-05-02
XINXIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The concentration of paraquat in plasma or serum is very low, at the level of μg / ml, the sensitivity of common biochemical detection methods and detection reagents is difficult to reach the detection limit
Furthermore, the matrix composition of the blood sample is complex, there are many influencing factors, and there are many interfering factors during the measurement, so the accuracy of the results is difficult to grasp.
Although some domestic scholars have tried to establish a simple UV assay, due to the influence of the above factors, the specific experimental conditions have not been refined, the reliability and stability of the results are poor, and it has not been widely used in clinical practice.
In addition, paraquat poisoning has extensive tissue damage, various clinical manifestations, and large individual differences. It is difficult to determine the exact relationship between the poison concentration and clinical manifestations and therapeutic effects.
Moreover, if there are many detection steps, large differences in reagents, and poor conditional stability, it is difficult to guarantee the performance of the detection results.

Method used

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  • Kit for rapid determination of paraquat concentration in blood
  • Kit for rapid determination of paraquat concentration in blood
  • Kit for rapid determination of paraquat concentration in blood

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0051] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Experimental example 1 kit and application method thereof

[0052] Instructions for the kit

[0053] [Scope of application and clinical significance] This kit is used for in vitro quantitative analysis of paraquat content in human serum and heparin / EDTA anticoagulated plasma. Paraquat is a commonly used herbicide, and paraquat poisoning is very common in clinical practice. Serum (serum) paraquat concentration detection can provide the most direct and objective basis for clinical diagnosis, treatment, disease detection and prognosis analysis of poisoned patients.

[0054] 【Determination principle】Paraquat molecules contain conjugated double bonds, which have stable absorption characteristics for 258nm ultraviolet rays. On the basis of removing protein, removing nucleic acid, and resisting the influence of reducing substances in the sam...

Embodiment 2

[0081] 【Reagent Composition】

[0082] The kit of the present invention mainly includes three parts: reagent I, reagent II and kit solvent, and each kit of reagents is prepared according to the amount of reagent for 60 people. It is also possible to appropriately increase or decrease the weight parts of each component according to the actual application, so as to meet different demands.

[0083] Reagent I is freeze-dried solid powder, containing: 1.8g of trichloroacetic acid, 0.23g of anhydrous sodium acetate, and 0.11g of sodium chloride;

[0084] Reagent II contains the following components: 9% PEG, 19mmol / L magnesium chloride, 0.22mol / L Tris buffer solution, adjusted to pH 9.8 with sodium hydroxide solution; reagent II is 45ml;

[0085] Kit solvent: double distilled water 45ml;

[0086] In addition, there are paraquat serum standards and paraquat serum calibrator: use the mixed serum of healthy people as the matrix, and prepare the application solution with the final conce...

Embodiment 3

[0094] 【Reagent Composition】

[0095] The kit of the present invention mainly includes three parts: reagent I, reagent II and kit solvent, and each kit of reagents is prepared according to the amount of reagent for 60 people. It is also possible to appropriately increase or decrease the weight parts of each component according to the actual application, so as to meet different demands.

[0096] Reagent I is freeze-dried solid powder, containing: 2.2g of trichloroacetic acid, 0.27g of anhydrous sodium acetate, and 0.13g of sodium chloride;

[0097] Reagent II contains the following components: 11% PEG, 21mmol / L magnesium chloride, 0.26mol / L Tris buffer, pH 10.0;

[0098] Kit solvent: double distilled water 55ml;

[0099] In addition, there are paraquat serum standards and paraquat serum calibrator: use the mixed serum of healthy people as the matrix, and prepare the application solution with the final concentration of 20μg / ml and 4μg / ml respectively.

[0100] 【Application meth...

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Abstract

The invention relates to a kit for rapid determination of paraquat concentration in blood. The kit mainly comprises a reagent I for removing proteins, a reagent II for removing nucleic acid and preventing interference, a kit solvent, a paraquat standard and a paraquat calibrator. The invention also relates to an application method of the kit. The kit can rapidly determine the paraquat concentration in the blood of a patient, and provides rapid, simple, accurate, reliable and effective reference for clinical diagnosis of paraquat intoxication.

Description

technical field [0001] The invention relates to a kit, in particular to a kit for rapidly detecting the concentration of paraquat in blood. Background technique [0002] Paraquat (PQ) is a widely used herbicide in agricultural production. It is easy to use, effective in weed control and cheap, and is loved by farmers. But if used improperly, it can cause paraquat poisoning. Paraquat poisoning has a high incidence rate, strong toxicity to the human body, and extensive damage. So far, there is no specific antidote, so the patient's prognosis is poor and the mortality rate is high. [0003] At present, most studies on paraquat poisoning focus on the pathogenic mechanism and clinical treatment. However, as the most objective and critical indicator for judging the degree of poisoning, observing the curative effect, and conducting prognosis analysis, there are few studies on the concentration of paraquat in plasma or serum, and there is a lack of practical methods and related ki...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/33G01N33/50
Inventor 李平法梁栋梁詹合琴李生莹田少华张婧婧刘俊扇王全志王辉贺志安
Owner XINXIANG MEDICAL UNIV
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