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Method for rapidly detecting paraquat in biological matrix sample

A biological matrix and paraquat technology, applied in the field of detection and analysis, can solve the problems of unavoidable chromatography, a large amount of harmful waste liquid, and lengthy separation process, and achieve the effects of simple workflow, simplified clinical diagnosis, and reduced operating costs

Inactive Publication Date: 2020-03-20
ASPEC TECH LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method requires complex pre-treatment and lengthy separation process, the analysis period is long and will produce a large amount of harmful waste liquid and pollute the environment
Mass spectrometry also cannot avoid some defects of chromatography itself due to the need for chromatographic separation

Method used

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  • Method for rapidly detecting paraquat in biological matrix sample
  • Method for rapidly detecting paraquat in biological matrix sample
  • Method for rapidly detecting paraquat in biological matrix sample

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Experimental program
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Effect test

Embodiment 1

[0028] Instruments and reagents

[0029] API 4000 triple quadrupole mass spectrometer (Applied BioSystem, USA), real-time direct analysis ion source (DART, IonSense, USA). Paraquat standard samples and experimental plasma came from the Academy of Military Medical Sciences, and stomach content samples came from the Forensic Medicine Center of Beijing Municipal Public Security Bureau.

[0030] 1. The main ions of paraquat generated by DART source in positive ion mode are: [M-H-2Cl] + (m / z185), [M+H-2Cl] + (m / z 187), [M+H-CH 3 -2Cl] + (m / z 172) and [M+H-2CH 3 Cl] + (m / z 157). Among them, both m / z 185 and 157 can be used as parent ions (see Table 1 for mass spectrometry parameters)

[0031] Table 1 Paraquat mass spectrometry DART-QqQ parameters

[0032]

[0033] Instrument conditions

[0034] DART-MS ion source parameters: positive ion mode; grid voltage 100V; injection track moving speed 0.4mm / s; heater temperature 500°C;

[0035] Mass spectrometry parameters: positive...

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Abstract

The invention discloses a method for rapidly detecting paraquat in a biological matrix sample, which uses a real-time direct analysis ion source and a mass spectrometer for detection, does not treat the detected biological matrix sample, and is an in-situ detection method. Pretreatment of traditional means is omitted, and rapid and real-time detection of biological matrix samples poisoned by paraquat is achieved.

Description

technical field [0001] The invention relates to a detection and analysis technology, in particular to a method for rapidly detecting paraquat in a biological matrix sample. Background technique [0002] Paraquat (paraquat), also known as Gramoxone, has a strong destructive effect on green plant tissue, and is used as a rapid, broad-spectrum, contact herbicide and defoliant in agriculture. Paraquat is extremely toxic, with an oral lethal dose of 40mg / kg, and there is no specific antidote. If ingested into the body by mistake, paraquat will enter target organs such as the lungs and kidneys, causing lung fibrosis and kidney failure. Although paraquat has little side effects on the environment, more and more poisoning incidents have made it a serious threat to human life and property. At present, more than 20 countries have banned or strictly restricted the use of paraquat. Because of its high toxicity and widespread application, it is extremely important to establish an effi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/72G01N30/06
CPCG01N30/72G01N30/06
Inventor 段晓琨刘春胜
Owner ASPEC TECH LTD
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