Patents
Literature
Hiro is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
Hiro

30 results about "Pancreas islet cell" patented technology

Methods for the induction of a cell to enter the islet 1+ lineage and a method for the expansion thereof

InactiveUS20110033430A1Enhancing the wnt/β-catenin pathwayIncreasing and enhancing wnt signalingBiocideGenetic material ingredientsProgenitorISL1
The present invention relates to methods for the induction and a cell to enter the Islet 1+ (Isl1+) lineage and methods for expansion of cells of islet 1+ lineage. One aspect of the present invention relates to methods to induce a cell to enter the islet 1+ lineage, and more particularly to a method to induce a cell to enter a the Isl1+ lineage to become an Isl1+ progenitor that is capable of differentiating along multiple different lineages such as a endothelial lineage, a smooth muscle lineage or a cardiac lineage. In particular, one embodiment present invention relates to methods to induce a cell to enter the Isl1+ lineage by inhibiting a wnt signalling pathway in the cell. Another aspect of the present invention relates to methods to expand a cell of the Isl1+ lineage, such as a Isl1+ progenitor by activating a wnt signalling pathway in the Isl1+ progenitor. Another aspect of the present invention relates to use of cells of the isl1+ lineage in subjects for therapeutic and preventative treatment of cardiovascular diseases.
Owner:THE GENERAL HOSPITAL CORP

Biscuit with function of curing diabetes and preparation method thereof

The invention relates to a biscuit with the function of curing the diabetes and a preparation method thereof. The preparation method of the biscuit comprises the steps of: taking fingered citron, Chinese yam, mulberry, medlar, semen coicis, sealwort, fructus citri and kudzu root as raw materials; mixing the components of the raw materials; decocting extracting solution by means of water adding, and respectively filtering by a sieve with 160-180 meshes; concentrating filter liquor to obtain extractum; modulating flourdough according to the weight ratio of the extractum to the flour at 1:8-15; and preparing the biscuit by an existing normal biscuit preparation process. After the biscuit is eaten, the pancreas islet cell can be nourished, the pancreas islet function can be improved, the glucogenic index of the food can be effectively controlled, the fasting blood-glucose and the postmeal glucose can be reduced, the use level of the drug for curing the diabetes can be reduced, and the life quality can be improved.
Owner:王钢柱

Reg III/proinsulin double-gene plasmid as well as building method and application thereof

The invention provides a plasmid for treating 1 type diabetes, which is a double-gene eukaryon co-expression plasmid built by a gene recombination method and containing a pancreas islet beta cell regeneration (Reg) III gene and a proinsulin gene, wherein the Reg III gene and the proinsulin gene can be respectively expressed under the induction of an EF-1alpha promoter and a cytomegalovirus (CMV) promoter of a pBudCE4.1 plasmid vector. The Reg III / proinsulin double-gene eukaryon co-expression plasmid can play a hypoglycemic role by recovering the autoimmunity tolerance state of in the body of a 1 type diabetes patient and promoting the regeneration of beta cells.
Owner:HUAZHONG UNIV OF SCI & TECH

Method for extracting, separating and purifying lyciumbarbarum polysaccharide, lyciumbarbarumglycopeptide and hypoglycemic medicament

The invention belongs to the technical filed of extraction, separation and purification; the invention discloses a method for extracting, separating and purifying lyciumbarbarum polysaccharide, lyciumbarbarumglycopeptide and a hypoglycemic medicament. The lyciumbarbarumpolysaccharide has effects in enhancing immunity, preventing aging, enhancing hematopoiesis, and preventing genetic injury; the lyceum barbarum polysaccharide (LBP) is a complex polysaccharide composed of acidic heteropolysaccharide and polypeptide or protein, and has glycopeptide structure of Glycano-O-Ser; the anti-tumor effect of the lyciumbarbarumglycopeptide is completed through an extremely complex regulation and control mode: the production of liver glycogen is inhibited by interfering the expression of related enzymes and proteins, so that the effect of reducing blood sugar is achieved. LBP has a positive effect on insulin and islet cells so as to indirectly achieve the effect of reducing blood sugar, LBP does not achieve the effect of reducing blood sugar by promoting pancreas to secrete insulin, and is a very good product for treating diabetes.
Owner:EAST CHINA UNIV OF SCI & TECH

Preparation method and application of scoparia dulcis extract for resisting islet cell apoptosis

The invention belongs to the technical field of medical biology, and particularly relates to a preparation method and an application of a scoparia dulcis extract for resisting islet cell apoptosis. According to the preparation method, the scoparia dulcis extract is prepared through the steps of heating reflux, extraction, silica gel column chromatography and the like. Cell experiments and animal experiments prove that the scoparia dulcis extract prepared by the preparation method disclosed by the invention has a remarkable anti-islet cell apoptosis effect and has a bright application prospectin the aspect of preparing medicines for resisting islet cell apoptosis.
Owner:HAINAN MEDICAL COLLEGE

Two labdane diterpenoids, and extraction method and application thereof

ActiveCN111285766ASignificant anti-apoptotic effect of islet cellsOrganic compound preparationMetabolism disorderBiotechnologyPancreas islet cell
The invention belongs to the field of biochemical medicines, and discloses two labdane diterpenoids, and an extraction method and application thereof. The structures of the two labdane diterpenoids are shown in the specification. The labdane diterpenoids are extracted and separated from scoparia dulcis l. Cell experiments prove that the labdane diterpenoids can remarkably resist pancreas islet cell apoptosis.
Owner:HAINAN MEDICAL COLLEGE

Traditional Chinese medicine composition for treating early diabetic nephropathy and preparation method thereof

The invention discloses a traditional Chinese medicine composition for treating early diabetic nephropathy, which is prepared by the following traditional Chinese medicine raw materials in percentage by weight: 10-15% of membranous milkvetch root, 5-7% of swordlike atractylodes rhizome, 10-15% of coix seed, 3-5% of tangerine peel, 5-7% of pinellia tuber, 7-15% of fortune eupatorium herb, 3-5% of officinal magnolia bark, 5-7% of hiraute shiny bugleweed herb, 5-10% of motherwort herb, 10-15% of mulberry fruit and 2-7% of leech. The traditional Chinese medicine composition can increase the sensibility of target cells to insulin, restore the functions of pancreas islet cells, promote glucose utilization of peripheral tissues, adjust an immune system, enhance the self-stable state of the glucose and improve the overall function of an organism; furthermore, the traditional Chinese medicine composition further has the effects of reducing blood lipid and urine protein.
Owner:李盈

Medicine for treating diabetes

The invention discloses a medicine for treating diabetes. The medicine comprises the following raw materials in percentage by weight: 11.1-15.1% of ginseng fruits, 11.1-15.1% of fragrant solomonseal rhizomes, 10.1-14.1% of kudzuvine roots, 10.1-14.1% of Chinese wolfberries, 10.1-12.1% of mulberry leaves, 9.1-13.1% of malt, 8.5-12.5% of astragalus roots, 8.5-12.5% of solomonseal rhizomes and 3.4-9.4% of coptis roots. According to the medicine for treating diabetes, through combination of the raw materials, immunity of organisms can be enhanced, virus infection can be resisted, and activity of the pancreas can be protected; normal metabolism of islet cells can be restored, and the secretion function of islet B cells can be improved; damaged islet B cells can be cultured for regeneration, insulin receptors can be enhanced, and the regulation effect of insulin can be fully exerted; trace elements closely related to diabetes can be highly supplemented; blood sugar and urine sugar can be rapidly reduced, and complications can be actively prevented and treated; and the health of diabetes patients can be comprehensively restored. The Tangshu capsule is only suitable for type II diabetes patients, is verified to have no toxic or side effects by toxicological experiments, is safe, convenient and reliable, has low dosage, is easily acceptable by patients, and has rapid and an obvious curative effect.
Owner:天津市泽陇生物科技有限公司

Artificial pancreas islet tissue and preparation and application thereof

The present invention relates to an artificial pancreas islet tissue and particularly comprises a biocompatible stent, islet cells and a mixed culture solution. Through a 3D bioprinting technique, thebiocompatible stent and the pancreas islet tissue are prepared into the biologically active artificial pancreas islet tissue. The mixed culture solution is used for culture and preservation of the pancreas islet cells and tissue. The biocompatible stent is prepared using the 3D printing technique. The artificial pancreas islet tissue can be used as a pathophysiological mechanism research and a therapeutic tool for diabetes.
Owner:SHENZHEN INST OF ADVANCED TECH

Method for inducing pancreas islet cells differentiated by stem cells in vitro to form pancreas islet-like structure

The invention discloses a method for in-vitro induction of stem cell differentiated islet cells to form islet-like structures, and relates to the technical field of biologication. The method comprises the following steps: enabling raw material tissue to be subjected to isolated culture to obtain mesenchymal stem cells; carrying out subculture on the obtained mesenchymal stem cells; and carrying out induced differentiation on the obtained passage mesenchymal stem cells to differentiate into islet-like cells. By adopting the specific induction culture medium and the steps of pretreatment and induction culture, the islet cells differentiated by the stem cells can be rapidly and efficiently induced in vitro to form the islet-like structure, and the method is high in induction rate, high in product cell quality and good in application effect.
Owner:多能干细胞再生医学科技(广州)有限公司

Method and kit allowing reverse differentiation of human somatic cells for generation of autologous pancreatic stem cells and autologous pancreas islet, and application thereof

InactiveCN102399740AAddressing critical deficitsImprove securityNervous disorderPancreatic cellsCell specificDisease
The invention provides a method allowing reverse differentiation of human somatic cells for generation of autologous pancreatic stem cells and autologous pancreas islet. The method is as follows: culture solutions containing extractives of different plants and different protein components are used to culture human somatic cells step by step, which enables the somatic cells to generate autologous pancreatic stem cells through reverse differentiation, and autologous pancreas islet is generated through further culture. The method provided in the invention enables the first generation of autologous pancreatic stem cells and autologous pancreas islet at a magnitude order of tens of billions to be generated within two to three weeks. The plant and protein-induced human autologous pancreatic stem cells have similar cell specific phenotypes and a plurality of cell differentiation capability as those of human natural pancreatic stem cells; the autologous pancreatic stem cells and autologous pancreas islet cells have immense potential both in the application field of treatment of pancreatic diseases like insulin deficiency type diabetes and in the engineering field of autologous pancreatic tissue; the invention is also applicable to establishment of novel autologous pancreatic stem cell libraries and long-term permanent preservation of autologous pancreatic stem cells.
Owner:林雄斌

Method for acquisition of sugar-reducing medicine and sugar-reducing medicine

The invention discloses a method for acquisition of a sugar-reducing medicine and the sugar-reducing medicine, and relates to the technical field of the medical treatment and public health. The method includes steps of through inhibiting inflammation, improving the insulin secretion capacity and the insulin resistance capacity of an early-stage INS-1 pancreas islet Beta cell line and a rodent experimental animal, and realizing the reduction of blood sugar, specifically using a pharmaceutical containing a p38 MAPK inhibitor ingredient SB203580 to realize. The provided sugar-reducing medicine is capable of preparing a pharmaceutical for nursing, delaying and / or treating diabetes or future application in a health care product. The p38 MAPK inhibitor SB203580 is capable of remarkably improving the insulin secretion capacity and the insulin resistance capacity of diabetes patient pancreas islet Beta cells, reducing the blood sugar level of the diabetes patient, and delaying and improving the development of the diabetes through inhibiting the inflammation, and has extensive application prospect in the treatment and nursing of the diabetes.
Owner:PEKING UNIV FIRST HOSPITAL

Flour with function of treating diabetes and preparation method thereof

The invention relates to flour with a function of treating diabetes and a preparation method thereof. The preparation method comprises the following steps of: 1, weighing the following components in part by weight: 10 to 13 parts of medlar, 12 to 15 parts of mulberry fruit, 20 to 25 parts of finger citron, 15 to 18 parts of cinnamomvine, 5 to 10 parts of common macrocarpium fruit, 5 to 10 parts of dwarf lilyturf tuber, 14 to 17 parts of siberian solomonseal rhizome and 10 to 12 parts of parslane herb; 2, mixing the components, adding water and decocting the mixture in water twice, wherein at the first time, water in an amount which is 10 times that of the mixture is added to decoct the mixture for 2 hours, and at the second time, the water in an amount which is 8 times that of the mixture is added to decoct the mixture for 1.5 hours; filtering decoction extract obtained at two times with a 160 to 180-mesh sieve, and concentrating filter liquor until the density is between 1.23 and 1.26 g / cm<3> to obtain extractum; drying the extractum under vacuum, crushing, and sieving with an 80 to 100-mesh sieve to obtain fine powder; and mixing the fine powder and flour uniformly according to weight ratio of 1:(7-10) to obtain the flour. The flour can nourish pancreas islet cells, improve pancreas islet function, reduce fasting and postprandial blood sugar and improve the living quality of patients who suffer from the diabetes.
Owner:王钢柱

Bioactive pancreas islet glucose regulating preparation and preparation method thereof

The invention provides a bioactive pancreas islet glucose regulating preparation and a preparation method thereof. The preparation is prepared from main materials including pancreas protein polypeptides and pancreas islet active cells of animals (such as pigs, cattle and sheep) and medicinal auxiliary materials. The preparation method includes: preparing high-activity pancreas islet cell coating liquid and trypsin polypeptide liquid from healthy animal pancreases through a series of integrated processes including separation, purification, multiplication culture, coating, ultrafiltration and the like; proportionally mixing, and adding a stabilizing agent and an absorbent; preparing into lyophilized powder through lyophilization, or preparing into enteric capsules. Pancreas islet active cells and trypsin polypeptides are directly replenished to bodies by biological means, the large quantity of pancreas islet active cells and trypsin polypeptides cultured in vitro are absorbed to give full play to a biological group effect in vivo, and accordingly the normal glucose metabolic level can be maintained, and diabetic complications can be effectively controlled.
Owner:张晓帆

Composite traditional Chinese medicine dropping pill for treating type-II diabetes and preparation method of composite traditional Chinese medicine dropping pill

The invention discloses a composite traditional Chinese medicine dropping pill for treating type-II diabetes. The composite traditional Chinese medicine dropping pill comprises the following components in parts by weight: 1-3 parts of coptis chinensis powder, 2-3 parts of rhizoma anemarrhenae powder, 1-2 parts of pseudo-ginseng powder, 0.01-0.03 part of papaverine, 0.02-0.04 part of aminophyllineand 1-3 parts of folium mori. The composite traditional Chinese medicine dropping pill for treating type-II diabetes, which is provided by the invention, comprises main body components, namely finelyselected pure traditional Chinese medicines coptis chinensis, rhizoma anemarrhenae, folium mori and pseudo-ginseng, the formula is reasonable, the components are matched mutually with a synergic function, blood vessel walls can be softened, blood vessels can be expanded, thrombocyte agglomeration is remarkably inhibited, blood viscosity is remarkably reduced, the blood amount is increased, pancreas islet cell blood supply is ensured, normal functions of pancreas islet cells are brought into play, pancreas islet of glucose is balanced, then symptoms of type-II diabetes is relieved, and thus patients are finally relieved from drug dependence. In addition, the composite traditional Chinese medicine dropping pill is capable of effectively relieving hypertension symptoms and is free of side effect on organs of human bodies, and clinical tests show that the effective rate of the dropping pill is greater than 85%, and the dropping pill has the potential of being a good medicine for treating patients suffering from type-II diabetes.
Owner:李日奎

A method for directed differentiation of autologous immune cells into islet cells

The invention provides a method for using autologous immune cells to differentiate into pancreatic islet cells. The method is to obtain immune cells from peripheral blood of patients, dedifferentiate them in vitro into induced pluripotent stem cells, and then differentiate into pancreatic islet cells through directed differentiation. Through the method of cultivating islet cells in the present invention, immune cells isolated from autologous peripheral blood are selected to induce directional differentiation into islet cells, and then transplanted to the same patient to reduce the patient’s immune rejection. In addition, there is no Tissue matching and ethical issues. In the present invention, when induced pluripotent stem cells (iPSCs) are directedly differentiated into islet cells, after 20-28 days of induced differentiation, the purity of the finally induced islet cells identified by DTZ staining is more than 62.4%.
Owner:SHANDONG XINRUI BIOTECH CO LTD

Method for differentiating peripheral blood stem cells into endoderm cells and application thereof

The invention belongs to the technical field of cell differentiation, and particularly relates to a method for differentiating peripheral blood stem cells into endoderm cells and application of the method. According to the invention, peripheral blood stem cells are subjected to induced culture in the presence of BM, Activin A, Wnt3a, TGF-b, LIF, TNF-a, IFN-a, vinterferron-alpha, beta, gamma, B-27 and N2, such that endoderm cells are obtained. The technology is a technical supplement for differentiating the endoderm cells into the islet cells, and the clinical application range of differentiating the stem cells into the islet cells and applying the stem cells to islet transplantation is widened. The technology is expected to solve the islet cell source problem and the islet transplantation heterologous problem, and the clinical effect of islet transplantation and the long-term survival of the graft are improved.
Owner:宫念樵

Application of ISL1 in preparation of medicine for inhibiting early ferroptosis of transplanted pancreas islet and cell

The invention relates to the field of pancreas islet transplantation, in particular to application of ISL1 in preparation of a medicine for inhibiting early ferroptosis of transplanted pancreas islet and a cell. Islet cells for overexpressing ISL1 are constructed by using the adenovirus to obtain islet cells for overexpressing ISL1. The expression change conditions of ferroptosis-related proteins (GPX4 and ACSL3) and apoptosis-related proteins are detected by using the overexpression and the knock-down of the ISL1 in the rat beta cell INS-1 through the lentivirus so as to determine that the ISL1 has the protection effect on the STZ induced INS-1 ferroptosis. The adenovirus of EcoRI MCS-ISL1 is utilized to construct rat and human islet cells for overexpressing ISL1, and it is clear that ISL1 inhibits transplanted islet ferroptosis by promoting expression of ACSL3 in the islet cells. A solid foundation is laid for improving survival of transplanted pancreas islet by genetically modified human pancreas islet cells and widely applying pancreas islet transplantation to clinic. The constructed cell can be used for experiments and pancreas islet transplantation possibilities.
Owner:丁小明

Application of GP73 inhibitor in preparation of medicine for treating diabetes

The embodiment of the invention relates to application of a GP73 inhibitor in preparation of medicine for treating diabetes. According to the embodiment of the invention, the inventor finds that GP73 plays a key role in blood glucose regulation, and particularly finds that soluble GP73 can be specifically combined with glucagon to form a compound, the blood glucose increasing function and the gluconeogenesis function of the glucagon are enhanced, and the half-life period of the glucagon is prolonged; the condition that soluble GP73 can activate generation of liver and / or kidney sugar and a gluconeogenesis signal channel in a manner of not depending on glucagon is found; and based on the found regulation effect of GP73 on blood glucose, the inventor also proves that the GP73 inhibitor can reduce the blood glucose level and glycosylated hemoglobin level of diabetic mice, has a protection effect on pancreas islet beta cells, and achieve an effect of treating diabetes through animal experiments.
Owner:BEIJING SUNGEN BIOMEDICAL TECH CO LTD

A neovascular construct for implanting into the subcutaneous tissue of a subject and its preparation method

The invention discloses a new blood vessel construct for implanting into subcutaneous tissue of a subject and a preparation method thereof, and relates to the technical field of polymer materials. The neovascular construct is a nanofiber catheter made of the polymer material by electrospinning, and the material is selected from one of L-polylactic acid, D-polylactic acid, polylactic-co-glycolic acid copolymer and polyglycolic acid , the vascular construct prepared by using the polymer material is transplanted into the subcutaneous tissue, which can quickly form an extracellular matrix space rich in new blood vessels in the subcutaneous tissue of the subject, which is used to accommodate the donor-derived islets or islet cells for long-term maintenance of function.
Owner:GUANGZHOU WOMEN AND CHILDRENS MEDICAL CENTER

Functional food suitable for diabetics and preparation method thereof

PendingCN113662188AMutual promotion and mutual complementation are goodFunction increaseOrganic active ingredientsMetabolism disorderSide effectTremella
The invention relates to a functional food suitable for diabetics and a preparation method thereof, and belongs to the technical field of functional foods. The technical problem that in the prior art, an insulin treatment method and a chemical medicine treatment method are large in toxic and side effects is solved. The functional food suitable for diabetics comprises the following components in parts by weight: 50 parts of ginseng, 50 parts of chitosan oligosaccharide, 50 parts of coptis chinensis, 100 parts of tremella, 100 parts of konjak and 150 parts of hericium erinaceus. The functional food suitable for the diabetics has the effects of reducing blood sugar, improving pancreas islet functions, repairing damaged pancreas islet cells and promoting the blood sugar reducing effect of insulin.
Owner:吉林省昆蓉科技有限公司

Traditional Chinese medicinal composition, traditional Chinese medicinal preparation, preparation methods and use thereof

ActiveCN101972316AMetabolism disorderPlant ingredientsSalvia miltiorrhizaBeta cell apoptosis
The invention relates to a traditional Chinese medicinal composition, a traditional Chinese medicinal preparation, preparation methods and use thereof and belongs to the technical field of traditional Chinese medicinal preparations. The traditional Chinese medicinal composition is prepared from the following raw materials: astragalus, vinegar-prepared corydalis tuber prepared, pseudo-ginseng root, red paeony root, Salvia miltiorrhiza Bunge, szechwan lovage rhizome, safflower, sappan wood and suberect spatholobus stem. The traditional Chinese medicinal preparation comprises active ingredients and a pharmaceutically acceptable carrier. The traditional Chinese medicinal composition and the traditional Chinese medicinal preparation have the functions of inhibiting or delaying islet beta-cell apoptosis and promoting the restoration of the functions of the islet beta-cells.
Owner:LIAONING AODA PHARM CO LTD

Double-cell co-packaging microgel for pancreas islet transplantation and preparation method of double-cell co-packaging microgel

The invention discloses a double-cell co-packaged microgel for pancreas islet transplantation and a preparation method thereof. The preparation method of the double-cell co-packaged microgel comprises the following steps: respectively synthesizing carbon-carbon double bond modified polymer materials; the preparation method comprises the following steps: dispersing pancreas islets and mesenchymal stem cells into a polymer precursor solution, preparing microgel by using a microfluidic technology, and initiating free aggregation and realizing internal crosslinking of the microgel through blue light, so as to prepare the microgel encapsulating the two cells. The microgel has good biocompatibility and can be used for entrapment of islet cells, the co-encapsulated mesenchymal stem cells can release immunoregulatory factors and inhibit immune cells, and a good immune tolerance environment is provided for cell transplantation.
Owner:SOUTH CHINA UNIV OF TECH

Application of FMO3 as target in screening of drugs or models for preventing and treating pancreas islet function damage

The invention belongs to the technical field of medicine, and relates to application of synthase FMO3 of intestinal flora metabolite TMAO as a target in screening of drugs or models for preventing and treating pancreas islet function damage. Specifically, the invention discloses application of FMO3 as a target in screening of drugs or biological agents for preventing and treating diseases caused by pancreas islet function impairment and application of FMO3 in preparation of drugs or biological agent models for screening of diseases caused by pancreas islet function impairment. According to the invention, FMO3 is taken as a target and is applied to screening of drugs for promoting glucose-stimulated insulin secretion. The product of FMO3 is TMAO, and a large number of tests prove that TMAO obviously inhibits insulin secretion stimulated by mouse pancreatic beta cell Min6 glucose, knocks down diabetic mouse FMO3, inhibits the level of TMAO, and can improve insufficiency of insulin secretion.
Owner:INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI

Islet cell and separation method thereof

The invention provides islet cells and a separation method thereof, and the separation method comprises the following steps: putting an isolated pancreatic tissue into a phosphate buffer salt solution, and pouring a collagenase solution into the treated pancreatic tissue at multiple parts, so that the pancreatic tissue is fully expanded to obtain a swollen pancreatic tissue; the method comprises the following steps: putting swollen pancreatic tissues into a collagenase-containing solution, centrifuging and digesting for multiple times, adding a buffer solution to terminate digestion, centrifuging, discarding supernatant, and resuspending to obtain a resuspension solution; and filtering, re-suspending the filtrate for multiple times to obtain a minced digestion end product, filtering to obtain a precipitate, blowing the precipitate into a culture dish, and collecting. When collagenase pancreas perfusion is carried out, multi-part slow injection is adopted, so that pancreatic tissues can be fully filled, the operation time is shortened, the operation is simple and convenient, the success rate is high, and pancreas islet injury caused by the fact that a collagenase solution is difficult to reach the interiors of tissue blocks and the digestion speeds inside and outside the tissues are inconsistent in the conventional chopping method is avoided; and the requirements of in-vitro basic experiments can be met.
Owner:ZHONGSHAN HOSPITAL FUDAN UNIV

Applications of adiponectin modified islet cells in improvement or enhancement of islet transplantation

The invention relates to the field of biological medicines, and in particular, to applications of adiponectin modification to enable islet cells to have antioxidant and / or anti-inflammatory characteristics and applications of adiponectin modification of the islet cells in improvement or enhancement of islet transplantation. The islet cells are subjected to adiponectin modification before islet transplantation, so that the islet cells overexpress adiponectin. The islet cells have the characteristics of oxidation resistance and inflammation resistance. Therefore, islet transplantation results are improved. During gene therapy, the islet cells are decomposed into single cells, so that the infection efficiency of viruses is greatly improved. After the gene therapy, the single cells are re-cultivated into functional cell clusters, so that pancreas islet functions are greatly reserved.
Owner:WEST CHINA HOSPITAL SICHUAN UNIV

Degradable immunomagnetic bead and preparation method thereof

The invention relates to the fields of nano magnetic materials and biology, and discloses a preparation method of degradable immunomagnetic beads. Comprising the following steps: preparing a degradable magnetic bead microsphere raw material, preparing alginate magnetic bead microspheres by adopting a rapid membrane emulsification technology and the degradable magnetic bead microsphere raw material prepared in the step S1, and coupling the alginate magnetic bead microspheres with an antibody; the invention also discloses a degradable immunomagnetic bead. The invention further discloses application of the degradable immunomagnetic beads in products for separating human islet cell clusters. The immunomagnetic bead prepared by the invention can be specifically targeted to the pancreas islet in human pancreas, can realize separation of pancreas islet cell mass and exocrine tissue, can greatly shorten the pancreas islet separation time, improve the separation efficiency, improve the activity and function of the pancreas islet cell mass, improve the transplantation effect, and indirectly reduce the cost of pancreas islet transplantation; the prepared immunomagnetic beads are low in toxicity, good in magnetic response and good in surface biocompatibility, and can be degraded under the condition that the pH value of a solution is high.
Owner:ZHEJIANG SHUREN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products