44 results about "Oxytetracyclinum" patented technology

The invention discloses a purification method of oxytetracycline, comprising the following steps of: (1) adding the oxytetracycline to be purified to alcohol, then adding a hydrochloric acid solution and stirring for complete dissolution; (2) cooling the solution obtained in the step (1) to -4 to 8 DEG C to separate out crystals, and then filtering; (3) dissolving the crystals obtained by filtering in the step (2) in high-purity water, adding a sodium bicarbonate solution, regulating the pH to 4-8 to separate out the crystals, and then filtering; and (4) drying the crystals obtained by filtering in the step (3) to obtain the purified oxytetracycline. The alcohol and the high-purity water are respectively used as solvents, and two-time crystallization is carried out to the oxytetracycline by combining the steps of acidification and neutralization, thereby the method greatly improves the purity of the oxytetracycline.

The invention relates to a preparation method of a silk screen printing sensor for detecting tetracycline and oxytetracycline at the same time, and belongs to the technical field of novel biological sensor detection. On the basis of the advantages that a silk screen printing electrode is high in conductivity, low in price, high in repeatability, convenient to carry, capable of implementing online detection and the like, the method can adapt to tests in different environments, the uncertain factors caused in the glassy carbon electrode grinding process are reduced, and therefore the good effect on detecting tetracycline and oxytetracycline can be achieved on the basis of the silk screen printing strategy.

The invention relates to a protective agent for relieving freezing damage to pears in a flowering season. Each part of agent comprises 180-270 thousand units of oxytetracycline or streptomycin, 1.1-1.6 grams of lycine, 0.08-0.12 gram of salicylic acid, 0.8-1.2 grams of borax, 40-60 milligrams of gibberellin, 0.18-0.21 gram of organosilicon, 2.5-3.6 grams of urea and 8-11 grams of white granulated sugar. Through the interaction of the raw materials, pear flowers are prevented from freezing damage. Thus, the protective agent has favorable antifreezing effect and achieves high fruit setting rate.

The invention discloses a method for regulating turfgrass heavy metal accumulation by using an OTC (oxytetracycline) and carbon nano-tube interlayer. An oxide-modified carbon nano-tube is used as an interlayer. The objectives are to reduce effect of leaking heavy metal to underground after the OTC is coupled with compost compound heavy metal, and to provide important technical support for a modified carbon nano-tube repairing heavy metal coupled pollution. Experiment results show that under the action of the OTC, adding of the carbon nano-tubes mixed in layers increases heavy metal accumulation amount of the overground part of festuca arundinacea, and heavy metal total amount in percolate in the modified carbon nano-tube interlayer is lower than that of control groups. The addition of the modified carbon nano-tube reduces risks that heavy metal is transferred to underground water to a certain degree, and provides technical support for practical application of the method.

The invention provides a preparation method of metacycline. The metacycline is prepared by carrying out dechlorination reaction on 11alpha-chloro-6-methyleneoxytetracycline p-toluenesulfonate under the action of a dechlorinating agent. The product prepared by the method is high in selectivity and yield, the yield of the metacycline is 90.8-98.6 percent, reaction conditions are very mild and safe,and reaction equipment model selection is relatively simple; on the other hand, the preparation of the dechlorination reagent is relatively simple, the reaction process is relatively simple, and the production cost is greatly reduced.

The invention discloses a preparation method for oxytetracycline hydrochloride. The preparation method comprises the following steps: (A) dissolving 20-30 Kg of anhydrous calcium chloride into 750-900 L of methanol solvent, adding 4-6 Kg of diatomaceous earth to facilitate filtration at the same time, stirring, and filtering a methanol solution to obtain a methanol-calcium chloride solution; (B) adding 5-8 L of high-purity hydrochloric acid into the methanol-calcium chloride solution, uniformly stirring, then adding 150-180 Kgof oxytetracycline dihyclorate, fully stirring for dissolution, adding 45-70 L of high-purity hydrochloric acid, and performing cooling crystallization to obtain oxytetracycline hydrochloride crystals; (C) placing the oxytetracycline hydrochloride crystals into a centrifuge, leaching the oxytetracycline hydrochloride crystals for four times with 200 L of methanol, performing centrifugation for 50 minutes after leaching, discharging, and performing fluidized drying, and after drying, cooling and discharging to obtain a oxytetracycline hydrochloride finished product. According to the preparation method, the oxytetracycline hydrochloride yield can be stabilized to be about 94 percent.

The invention provides a pharmaceutical composition for alleviating vomiting reaction caused by oxytetracycline as well as a preparation and detection method of formula granules and application thereof and belongs to the field of medicines. The pharmaceutical composition comprises uridine, glycyrrhizic acid, rutin, liquiritin and the like. The preparation method comprises the following steps: extracting, filtering, concentrating and drying licorice decoction pieces so as to obtain the composition, mixing, pelletizing, finishing, total blending and packaging the composition with ingredients, thereby obtaining formula granules. The formula granules are excellent in stability and high in quality controllability, and the vomiting reaction caused by oxytetracycline can be obviously alleviated.

The invention provides preparation of a boron nitride material anchored cobalt ferrite composite catalyst and application of the boron nitride material anchored cobalt ferrite composite catalyst in catalytic degradation of oxytetracycline. According to the preparation method, the boron nitride material anchored cobalt ferrite composite catalyst BNMTs (at) CoFe2O4 and H-BN (at) CoFe2O4 are prepared by adopting a coprecipitation and hydrothermal method. According to an advanced oxidation process based on activated peroxymonosulfate (PMS), the composite catalyst is used for degrading oxytetracycline, and H-BN (at) CoFe2O4 / PMS and BNMTs (at) CoFe2O4 / PMS systems both show good degradation performance and cycling stability on the oxytetracycline. The BNMTs-coated CoFe2O4 has abundant mesopores and oxygen-containing groups, so that CoFe2O4 is more effectively fixed and dispersed, the activation of PMS is remarkably enhanced, and the degradation performance on oxytetracycline is more excellent. The method is expected to provide more thoughts for reasonable design of a boron nitride material and a transition metal activated PMS system and application in the aspects of removal of refractory organic wastewater and the like.

The invention discloses a long-acting oxytetracycline injection and a preparation method of the long-acting oxytetracycline injection, and belongs to the technical field of veterinary preparations. Every 1000ml of the injection comprises the following components: 100-240 g of oxytetracycline, 120-140 g of sodium formaldehyde sulfoxylate, 300-400 g of propylene glycol, 80-120 g of magnesium chloride, 10-50 ml of ethanol amine, 50-100 g of pullulan-polyglutamic acid cross-linked polymer, 10-20 ml of honeysuckle extracting solution, 10-20 ml of buckwheat tea extracting solution, and the balance of water for injection. The product quality is stable, the long-acting oxytetracycline injection does not change the color and does not precipitate in the guarantee period, the drug effect lasts for 4 days by injecting once, and the long-acting oxytetracycline injection has better treatment effects on treating doldrums and hyperthermia caused by eperythrozoonosis, swine enzootic pneumonia, mycoplasma and rickettsial diseases of pigs, as well as high fever and systemic diseases.

The invention relates to the technical field of medicine preparation and particularly discloses a medicine for treating burn and scald. The medicine is prepared according to the method which comprises the following steps: mixing oxytetracycline tablets with furazolidone tablets according to the weight ratio of 1:2 and crushing into powder, and adding a little of vegetable oil and stirring into a thick liquid, thus obtaining the medicine, wherein the vegetable oil may be camellia oil, olive oil, peanut oil or soybean oil. The medicine for treating burn and scald has a cure rate of 100%, and free from any side effect; compared with other medicines in same type, the medicine is simple to get materials, convenient and rapid to produce, low in cost, easy to produce on a large scale, fast to take effect, good in curative effect to skin healing, and basically free from scars.

The invention relates to a preparation method of chlorinated oxytetracycline. The preparation method can be widely applied to the technical field of veterinary drugs and solves the technical problemsof large toxic and side effects, large environmental harm, high waste liquid treatment cost and poor intermediate product stability in the existing preparation method of chlorinated oxytetracycline. The reaction equations are as follows: a first-step chemical reaction equation; and a second-step chemical reaction equation.

The invention provides a fluorescent palladium nano-cluster and a synthesis method and application thereof. The palladium nano-cluster is obtained through the following preparation method which comprises the steps that with palladium nitrate as a raw material, polyethylenimine as a stabilizer, and ascorbic acid as a reducing agent, through a chemical reduction method, the fluorescent palladium nano-cluster is prepared at one step. The palladium nano-cluster has excellent fluorescence emission performance in an aqueous solution and good light-resistant bleaching performance and stability, and the solution is irradiated under a 365nm ultraviolet lamp to emit bright blue fluorescence. The invention further provides a method for detecting oxytetracycline by adopting the palladium nano-cluster.The oxytetracycline is detected through a fluorescence analysis method, the fluorescence intensity of the palladium nano-cluster is gradually lowered with increase of the concentration of the oxytetracycline, and the method has good selectivity. The detection method is easy to operate, capable of achieving high detection speed and greatly shortening the detection time, good in selectivity and lowin cost.

The invention discloses a method for simultaneously detecting the relative retention time and the peak purity of doxycycline and related substances in doxycycline hydrochloride, and belongs to the technical field of detection of medicinal finished products. The method is characterized by adopting a mode of combining HPLC (High Performance Liquid Chromatography) and DAD (Diode Array Detector) to simultaneously detect the relative retention time and the peak purity of doxycycline and related substances in doxycycline hydrochloride; wherein the HPLC adopts a UV detector, the UV detector and the DAD are arranged in series, a sample is firstly subjected to qualitative detection through the UV detector, and then subjected to peak purity detection through the DAD; the related substances include beta-doxycycline, metacycline, and oxytetracycline. The method of the invention can detect the peak purity values of the doxycycline, the beta-doxycycline, the metacycline and the oxytetracycline whiledetecting the relative retention time of the doxycycline and the related substances in the doxycycline hydrochloride.

The invention discloses an oxytetracycline wastewater treatment process. The oxytetracycline wastewater treatment process includes steps that oxytetracycline wastewater is filtered by a centrifugal machine and subjected to adsorption through adsorption resins, the potential of hydrogen (PH) value is adjusted to 6-7, filtering is performed by a precise filter which is 1 mum in precision, a common reverse osmosis membrane system is used for concentration, an electroosmosis system is used for desalination, a reverse osmosis (RO) concentrated solution serves as water supply of an electroosmosis fresh water chamber, a large part of RO dialysate directly reaches the standards to be discharged, a small part of the dialysate serves as a water supply of an electroosmosis concentrated water chamber and an electrode water chamber, inorganic salt in a fresh water chamber concentrated solution is continuously moved to the concentrated water chamber under the action of an electroosmosis electric field, a fresh water chamber low-pressure RO concentrated solution after desalination is delivered into a high-pressure RO system to be subjected to concentration to obtain a high-pressure RO concentrated solution, high-pressure RO dialysate reaches the standards to be discharged, and finally, evaporation is performed in an evaporation process. By means of the oxytetracycline wastewater treatment process, water outlet COD is smaller than 100mg / L, discharging up to standards can be directly performed, environmental protection can be achieved, and costs are saved.

The invention discloses a method for cooperatively controlling garden soil enzyme activity by adopting OTC (oxytetracycline) and compost. According to the method, the OTC and the urban refuse compost are utilized for cooperatively controlling the soil enzyme activity of gardening modeling plants, so that the aims of inhibiting the growth of ornamental plants in the gardening plant landscape and reducing the management cost are realized. The steps are mainly as follows: sowing 0.25 g of festuca arundinacea seeds, which are soaked for 24 hours in advance, on half of a vessel, which is filled with 125g of garden soil, 25 g of compost and OTC of 20 mmol / kg, respectively sampling the soil in a cup when the OTC is exposed, namely on the seventh day, the twenty-first day and the thirty-fifth day after seed germination, and carrying out measurement analysis on the activity of urease and catalase in the soil.

The invention belongs to the field of medicine, and particularly relates to an oxytetracycline preparation and a preparation method. The oxytetracycline preparation is characterized by comprising, by weight, 30% of oxytetracycline, 2-3% of magnesium chloride, 45-55% of 2-pyrrolidone, 2-5% of cholamine, 0.5-2% of sodium formaldehyde sulfoxylate and the balance water. The oxytetracycline preparation and the preparation method have the advantages that the complexation technology is adopted for preparation of the oxytetracycline preparation, so that the oxytetracycline preparation has stable quality, muddy sediments are not generated, slow release after injection can be achieved, and curative effect is improved; the preparation method is simple, easiness in production operation is achieved, production time is shortened by one hour as compared with common production time, production efficiency is higher, and production cost is reduced.

The invention discloses a composite callus ointment. The composite callus ointment is characterized by being prepared from the following components by ratio: 6.7% of oxytetracycline, 6.7% of berberine, 6.7% of Yunnan Baiyao, 3.3% of metronidazole, 3.3% of vitamin C, 3.3% of cimetidine, 2.3% of complex vitamin B, 1% of borneol and 66.7% of vaseline. The problems that an existing external ointment is poor in treatment effect on pain and itching caused by anorectal disease and chapped skin is solved; and the composite callus ointment is suitable for being smeared by patients with anal pruritus, anal fissure and chapped skin.

The invention discloses veterinary long-acting oxytetracycline injection liquid and a preparation method thereof. The veterinary long-acting oxytetracycline injection liquid contains oxytetracycline dihydrate, magnesium oxide, sodium formaldehyde sulfoxylate, glycerol formal, polyvinylpyrrolidone K12, polyethylene glycol-400, monoethanolamine and water for injection. According to the veterinary long-acting oxytetracycline injection liquid, the combined action of the oxytetracycline dihydrate with the low impurity content and the high purity, the sodium formaldehyde sulfoxylate, the magnesium oxide and the glycerol formal improves stability of the injection liquid, and the storage time of the injection liquid is prolonged; after the polyethylene glycol-400 is adopted to conduct intramuscular injection, the blood concentration peak can be reached in short time, so that intramuscular injection is easier; and by adopting the polyvinylpyrrolidone K12, drugs are slowly released, the drug action time is prolonged, the injection interval time is prolonged, the injection dosage is decreased, and animal stress response is reduced.

The invention belongs to the technical field of biosensors, and discloses a preparation method of ZIF-8-derived ZnO / g-C3N4 and an application of the ZIF-8-derived ZnO / g-C3N4 in an oxytetracycline sensor. ZIF-8-derived ZnO and g-C3N4 are combined, and due to the fact that the ZnO and the g-C3N4 have matched energy band structures, a typical type II heterojunction can be obtained through compounding, and then a relatively large photoelectric signal is obtained. The surface of an electrode is modified with the composite material, an oxytetracycline aptamer is further introduced, the high-performance photoelectrochemical biosensor is designed and constructed to achieve specific detection of oxytetracycline, the sensor has a good linear relation within the concentration range of 0.005-200 nM, and the detection limit is 3.92 * 10 <-4 > nM. The photoelectrochemical biosensor constructed by the invention is low in background signal, high in sensitivity and good in selectivity, and provides a good sensing platform for oxytetracycline detection.

The invention relates to the technical field of veterinary drugs, in particular to a preparation method of doxycycline intermediate 11[alpha]-chloromethene oxytetracycline. According to the preparation method, 11[alpha]-chloromethene oxytetracycline is taken as a raw material, dichloromethane is used as a solvent, and 1,1-dichloromethyl-N,N-dimethylamine is taken as a dehydrating agent to carry out reactions to obtain 11[alpha]-chloromethene oxytetracycline. By utilizing the preparation method, the production potential safety hazard can be reduced, the product yield is high, the generated waste salt is reduced, and the equipment type selection requirement is reduced.

The invention provides a preparation method of doxycycline hydrochloride. The preparation method of the present invention comprises: 11a-chloro-6-methine oxytetracycline p-benzenemethanesulfonate hydrogenation reaction under catalyst and poison conditions; then, react with sulfosalicylic acid to obtain α-6-deoxyoxytetracycline Sulfosalicylate; after reacting with hydrogen chloride-ethanol solution, add water to stir and crystallize to obtain doxycycline hydrochloride; wherein, the poison includes: a mixture of sulfur-containing and nitrogen-containing organic compounds. The method of the invention can improve the doxycycline hydrochloride yield and stereoselectivity, and obtain high-quality raw materials.

The invention provides an aptamer electrode for oxytetracycline detection, which is characterized in that the glassy carbon electrode is sequentially decorated with graphene-gold nanocomposites and BSA-OTC complexes from bottom to top. The signal layer is a nucleic acid aptamer modified by methylene blue and folded into a G quadruple structure under the RCA chain amplification reaction. The content of the target is reflected by the amount of methylene blue, and its preparation method is provided at the same time. The preparation method of the present invention is simple and has high performance. It is stable and has good electrode repeatability, and is suitable for the detection of oxytetracycline in drug safety and the practical application of biosensor industrialization.

The invention discloses a feed additive which is prepared by mixing the following components in parts by weight: 60 parts of corn, 10 parts of oxytetracycline, 3 parts of ferrous sulfate, 3 parts of shellfish powder, 4 parts of chitosan, 20 parts of wheat, 20 parts of bean pulp and 1 part of saccharin. According to the invention, the raw materials are low in price and have no toxic or side effect; and the feed additive is simple in production process, low in production cost, reasonable in mixture ratio and balanced in nutrition, and is easy to implement popularization and application.

The invention belongs to the technical field of environmental monitoring, and relates to an oxytetracycline fluorescence detection method based on surface passivation and covalent coupling. The covalent coupling between the surface of two-dimensional MOF nanosheets and DNA can effectively utilize the limited adsorption sites on the surface of MOF nanosheets, enhance the ability of nanosheets to adsorb DNA molecules, and reduce the background fluorescence signal. At the same time, the use of short-chain DNA molecules as signal reporter molecules can effectively improve the fluorescence recovery efficiency, while the addition of surface passivator sodium polystyrene sulfonate in the reaction system further weakens the adsorption ability of nanosheets to signal reporter molecules, making MOF nanosheets The chip sensor improves the linear response to low concentration OTC and enhances the detection performance of OTC. The detection method of the invention can effectively reduce the fluorescence background value, increase the signal response value induced by the target, and effectively improve the detection performance of the fluorescent sensing platform constructed based on the principle of physical adsorption for oxytetracycline.

The present invention provides a kind of preparation method of doxycycline hydrochloride and the doxycycline hydrochloride prepared by this method, provide raw material through the reaction of chlorinated antipyretic ice and oxytetracycline, improve the productive rate of doxycycline hydrochloride, The generation of by-products is reduced, and the antipyretic ice, ethanol, methanol, etc. in the preparation process can be recycled, environmental pollution and resource waste are reduced, and the production cost is reduced, and the product yield in the preparation process of the method is stable. In addition, the doxycycline hydrochloride prepared by the preparation method of the present invention has high purity and good color.

The invention discloses a high-content oxytetracycline injection and a preparation method thereof, belonging to the field of oxytetracycline injections for veterinary use. Every 100 mL of the oxytetracycline injection comprises 30 g to 35 g of oxytetracycline, 2.0 g to 3.0 g of magnesium oxide, 0.3 g to 1.0 g of sodium formaldehyde sulfoxylate, 20 g to 40 g of glycerolformal, 20 g to 40 g of N,N-dimethylacetamide, and 1.5 g to 2.0 g of monoethanolamine, with the balance being water for injection. The preparation method comprises the following steps: (1) adding a proper amount of water for injection to dissolve sodium formaldehyde sulfoxylate; (2) adding magnesium oxide and carrying out stirring to uniformly disperse the magnesium oxide; (3) continuing adding glycerolformal, N,N-dimethylacetamide and monoethanolamine; (4) introducing nitrogen, carrying out heating to 50-60 DEG C, adding oxytetracycline alkali, and carrying out stirring to dissolve the oxytetracycline alkali; (5) addingmonoethanolamine to adjust the pH value of the obtained solution to 8.4-8.7; and (6) supplementing water for injection until a volume is 100 mL. According to the invention, an optimal ratio is selected by screening the ratios of glycerolformal to dimethylacetamide; and it is verified that the oxytetracycline injection is good in stability, not prone to discoloring and content reduction after beingplaced for a long time, and free of sediments.

The invention belongs to the technical field of biosensors, and particularly relates to a preparation method and application of a photoelectrochemical biosensor for detecting oxytetracycline. Bi2S3 with the advantages of good chemical stability, narrow band gap and the like and a CN phase are compounded through a water phase synthesis method to construct a novel high-performance photosensitive composite material-direct Z-type heterojunction, and a photoelectric signal amplification mechanism is achieved; according to the present invention, the terramycin aptamer is further introduced, such that the specific recognition of the terramycin and the aptamer is achieved so as to construct the high-performance photoelectrochemical biosensor, the specific detection of the terramycin is achieved, the sensor has the good linear range within the range of 0.001-1000 nM, and the detection limit is 0.6 pM; the photoelectric biosensor constructed by the invention is low in background signal, high in sensitivity and good in selectivity, and provides a good sensing platform for detecting oxytetracycline.

The invention relates to a colorimetric aptamer sensor based on nanogold-DNA and a method for detecting oxytetracycline by using the colorimetric aptamer sensor, and belongs to the technical field ofenvironmental monitoring. The colorimetric aptamer sensor provided by the invention comprises nanogold and an OTC aptamer. The method for detecting oxytetracycline by applying the colorimetric aptamersensor comprises the following steps: 1, preparing the nanogold; 2, preparing the OTC aptamer; 3, mixing the nanogold and the OTC aptamer. The colorimetric sensor designed by the invention has the advantages of simplicity and convenience in operation, short detection time, high sensitivity, good selectivity, low detection limit, macroscopic property and the like, and is superior to other traditional OTC detection methods. The aptamer sensor disclosed by the invention can be successfully used for detecting oxytetracycline in an actual egg sample and detecting a recovery rate result of OTC in the egg sample, so the detection result is satisfactory; and a certain reference is provided for applying an aptamer-nanogold colorimetric method to detection of small molecules in foods.

The invention discloses a method for regulating and controlling compound heavy metal forms of municipal refuse compost by adopting OTC (oxytetracycline). According to the method, a PVC (polyvinyl chloride) pipe, of which the diameter is equal to 3cm, the height is equal to 25cm, is adopted, and 160 g of compost is added into the pipe; 40ml of aqueous solution with the mass concentration of 10 to 20 mmol / kg is added into the compost, wherein the temperature is 19 to 27 DEG C, and the relative humidity is 60% to 72% during the test, and water is supplemented to the compost every day under a natural light condition. A weighing method is adopted for adjusting water content, so as to keep the constant of the water content and make the water content in compost substrate reach to the maximum water-holding capacity of 70%, after curing for 45 days, sampling is carried out to analyze the forms of heavy metal. Experimental results show that after adding the OTC, the ratios of the exchangeable forms of Cd, Cr, Cu, Pb and Zn to the bound form of carbonate are all remarkably increased. By adding the OTC, the bio-available forms of the heavy metal in the compost are increased, and the non-bio-available forms are reduced, and activation is realized for the heavy metal in the compost.