141 results about "Hepatitis E virus" patented technology

The invention relates to an enantiomorphic amine alkyl sesquiterpene alcohol and glucoside and the medicated salt or solvent thereof, as well as the effect and activity of the composed medicine combination, mainly relating to the medical use in reducing HBV-DNA replication activity. And it has considerably strong inhibiting effect on HBsAG screted by HepG2.2.15 and HBV-DNA replication as compared with positive contrast Lamivudine; and it has obvious inhibition activity to HBV-DNA replication at large dosage (100 mug / mL) and medium dosage(20 mug / mL) as contrasted with Lamivudine, and can be expected to apply to preparing medicines for curing HB virus infection disease.

Herein disclosed are rapid real-time isothermal multiplex methods of detecting, identifying and quantifying bacterial, viral, and protozoan nucleic acids in a sample. These include contacting the sample with two or more sets of pathogen-specific reverse transcription loop-mediated isothermal amplification primers and novel oligofluorophores specific for the target bacterial, viral, and parasitic nucleic acids of interest such as human immunodeficiency virus, Ebola virus, Marburg virus, Yellow fever virus, hepatitis-B virus, Lassa fever virus, Plasmodium, hepatitis-C virus, hepatitis-E virus, dengue virus, Chikungunya virus, Japanese Encephalitis virus, Middle Eastern Respiratory Syndrome Corona virus, Mycobacterium, West Nile virus, Cytomegalovirus, Parvovirus, Leishmania, Trypanosoma, and Zika virus nucleic acids, under conditions sufficient to produce detectable real-time amplification signals in about 10 to 40 minutes. The amplification signals are produced by pathogen-specific fluorogenic labels included in one or more of the primers. Also, novel reaction and sample lysis buffers, primers, and kits for rapid multiplex detection, quantification, and identification of bacterial, viral, and protozoan nucleic acids by real-time isothermal amplification are herein disclosed.

HBV surface antigen particles, prepared by recombinant DNA technology are described, said particles being composed of epitopes from the group of surface peptides and / or core peptide of non-A, non-B hepatitis virus, hepatitis virus A and / or hepatitis virus B. Respective particles are especially characterized by a composition of different epitopes selected from pre-S and S peptides. There are also described DNA-sequences, plasmids and cell lines coding for respective HBV surface antigen particles as well as a new vaccine containing the same.

The traditional pig source hepatitis E has no effective vaccine for prevention, which adopts a control measure that the finding is carried out as soon as possible and the epidemic condition in epidemic areas is monitored at any time to block the spread of the disease. The invention discloses a rapid detection method for identifying each gene type of hepatitis E virus by utilizing multiple fluorescence quantitative PCR, which is characterized in that the a pair of conservative amplification primers and 4 strip-shaped specificity TaqMan probes are designed aiming at an HEVORF 3 sequence; the four probes are respectively designed aiming at the respective ORF 3 metamorphosis region sequence in a type specificity mode; and the identification and the detection of different gene types can be realized. The invention maintains the characteristics of high sensitivity and high accuracy of a PCR method and has the advantages that the quadruple fluorescence quantitative PCR enhances the detection efficiency and reduces the detection cost; the purposes of identification and diagnosis can be simultaneously realized; and the invention lays a foundation for work of infection source survey, spread environment, virus source tracing, etc.

The invention discloses a hepatitis E virus IgG antibody detection kit. The kit includes the following components: a solid material coated with a hepatitis E virus (HEV) gene recombinant antigen, a mouse anti human IgG monoclonal antibody labeled with a signal generation substance, a concentrated washing solution, an enhancing liquid, an analysis buffer liquid, a sample diluent, a positive control and a negative control. In addition, the invention also provides a preparation method of the kit. The preparation method includes the following steps: a first step, preparing the solid material coated with the HEV gene recombinant antigen; and a second step, labeling a mouse anti human IgG monoclonal antibody with the signal generation substance. In addition, the invention also discloses an application of the kit in detection of a hepatitis E virus IgG antibody. The detection kit overcomes the defect of labeling antibodies with macromolecules (such as enzymes), has the advantages of high sensitivity, good stability, low cost and the like, can significantly improve the specificity, sensitivity and stability of detection of the hepatitis E virus IgG antibody, and besides, significantly reduces the cost.

The invention relates to a hepatitis virus in-vitro culture model as well as a construction method and application thereof. The hepatitis virus in-vitro culture model is constructed by adopting human fetal liver stem cells and comprises the steps of carrying out separate culture on human fetal liver stem cell, immunohistochemically identifying human fetal liver stem cell and infecting human fetal liver stem cell with hepatitis virus by blood serum and verifying whether hepatitis virus infects the human fetal liver stem cells or not and the virus propagate and continuously secrete outside the cell or not; the model can be used for the screening and effect evaluation of an in-vitro hepatitis virus resistant medicament; the human fetal liver stem cells used in the hepatitis virus in-vitro culture model can be infected with hepatitis virus and can continuously secrete hepatitis virus; the human fetal liver stem cells can be subcultured; and the invention has simple and convenient method and high repeatability.

The invention relates to two monoclonal antibody hybridoma cell lines which secrete avian and porcine hepatitis e virus (HEV) shared epitope by virtue of a conventional hybridoma technique, wherein the lines are named 3E8 and 1B5 and the cell preservation numbers are respectively CCTCC No: C201395 and CCTCC No: C201396. The amino acid sequences of the shared epitope of the two lines identified by two monoclonal antibodies are identified by using a phage random 7 peptide library kit. The amino acid sequences are respectively V / IPHD (SEQ ID No: 1) and VKLYM (SEQ ID No: 2). Moreover, application of two mimic peptides of epitope in avian and porcine HEV serological diagnosis is analyzed. The two mimic peptides of the shared epitope of avian and porcine HEV capsid proteins disclosed by the invention can be used for rapid diagnosis of avian and porcine HEV and monitor of prevalence of disease. In addition, the two lines disclosed identify the monoclonal antibodies of the shared epitope and can be further used for rapidly diagnosing avian and porcine HEV.