The invention provides a fluorescent quantitative PCR detecting kit for a
hepatitis E
virus. The detecting kit comprises a PCR reaction solution, wherein the PCR reaction solution comprises a PCR buffering solution,
deoxyribonucleoside triphosphate,
a DNA polymerase, an upstream primer, a downstream primer and a probe, wherein the upstream primmer and the downstream primer are used for amplifying target
polynucleotide, and the probe is used for detecting the target
polynucleotide. The fluorescent quantitative PCR detecting kit for the
hepatitis E
virus is favorable in specificity, high in operation speed, simple and convenient in method and wide in detection range, and meanwhile an interior
label additionally arranged in the
reaction system can be used for effectively avoiding that the detected result is false negative. When the fluorescent quantitative PCR detecting kit for the
hepatitis E
virus is used for extracting
RNA, a
magnetic bead method which is good in
adsorption effect and easy to purify is used, and the method can be used for effectively removing PCR inhibitors in complex samples, so that
RNA with high purity and high yield is obtained, and detecting sensitivity, accuracy and stability are greatly improved.