40 results about "Human parvovirus" patented technology

Nucleic acid oligomers specific for human parvovirus B19 genomic DNA are disclosed. An assay for amplifying and detecting human parvovirus B19 nucleic acid in biological specimens is disclosed. Compositions for detecting the presence of parvovirus B19 genomic DNA in human biological specimens are disclosed.

Human parvovirus B19 primers and probes derived from conserved regions of the parvovirus B19 genome are disclosed. Also disclosed are nucleic acid-based assays using the primers and probes.

The invention relates to infectious clones of parvovirus B19, methods of cloning infectious B19 clones, and methods of cloning viral genomes that have secondary DNA structures that are unstable in bacterial cells. A B19 infectious clone and methods of producing B19 infectious clones are useful for producing infectious virus. Infectious virus is useful for identifying and developing therapeutically effective compositions for treatment and / or prevention of human parvovirus B19 infections.

Provided herein are sequences of the genomes and encoded proteins of a new human parvovirus, Bocavirus-2, and variants thereof. Also provided are methods of detecting the Bocavirus-2 and diagnosing Bocavirus-2 infection, methods of treating or preventing Bocavirus-2 infection, and methods for identifying anti-Bocavirus-2 compounds.

The invention provides a B19 (Human parvovirus B19), HTLV (Human T-cell lymphotropic virus) and HEV (Hepatitis E virus) quadruple fluorescent PCR (polymerase chain reaction) quick hypersensitivity detection kit and an application thereof, and relates to a real-time PCR method for quadruple detection of target nucleic acid in a nucleic acid extraction liquid in a single PCR reaction container. The method is used for detecting B19, HTLV-I, HTLV-2 and HEV.

The invention provides a codon-optimized parvovirus polynucleotide composition and methods of expressing this polynucleotide in a variety of mammalian cells, including non-erythroid progenitor cells, to produce immunogenic compositions.

The invention discloses a kit for detecting a human parvovirus IgM antibody. The kit comprises a B19-IgM magnetic particle suspension, a magnetic particle B19-IgM enzyme conjugate, a magnetic particleB19-IgM sample diluent, a magnetic particle B19-IgM calibration product, a light-emitting substrate and a concentrated washing solution, wherein a solid phase carrier in the B19-IgM magnetic particlesuspension is magnetic particles with the carboxylation particle size of 0.05-1 micron, a coated antibody is a mouse anti human IgM mu-chain monoclonal antibody, and an enzyme-labeled antigen of themagnetic particle B19-IgM enzyme conjugate is a horse radish peroxidase-labeled gene recombinant antigen. The kit has the advantages of being capable of carrying out detection analysis on a sample byutilizing a full-automatic chemiluminescent analyzer, simple and convenient to operate, short in detection time, accurate in detection result, wide in detection linear range and the like.

The present invention relates to the discovery of a new human parvovirus, methods of detecting the parvovirus and diagnosing parvovirus infection, methods of treating or preventing parvovirus infection, and methods for identifying anti-parvoviral compounds.

The invention discloses an RCA method for detecting human parvovirus B19. The method is implemented by the following steps: (1) extracting specimen DNA; (2) implementing RCA, then conducting gel electrophoresis by virtue of 1.0% agarose, and observing an electrophoresis result by virtue of a gel imaging instrument. The RCA method provided by the invention, which combines a padlock probe and an RCAtechnology, is a novel method for detecting the human parvovirus B19; by virtue of the padlock probe, the specific recognition of a target gene sequence is implemented; and by virtue of the RCA, detection signals are amplified. The method is free from the use of special equipment and the method is simple to operate, good in specificity and high in sensitivity; and the method has unique advantagesin the aspect of virus detection.

The invention provides a B19 (Human parvovirus B19), HTLV (Human T-cell lymphotropic virus) and HEV (Hepatitis E virus) quadruple fluorescent PCR (polymerase chain reaction) quick hypersensitivity detection kit and an application thereof, and relates to a real-time PCR method for quadruple detection of target nucleic acid in a nucleic acid extraction liquid in a single PCR reaction container. The method is used for detecting B19, HTLV-I, HTLV-2 and HEV.

Nucleic acid oligomers specific for human parvovirus genomic DNA are disclosed. An assay for amplifying and detecting human parvovirus genotypes 1, 2 and 3 nucleic acid in biological specimens is disclosed. Compositions for amplifying and detecting the presence of human parvovirus genotypes 1, 2 and 3 genomic DNA in human biological specimens are disclosed.

The invention discloses a detection kit for a human parvovirus B19 (HPVB19) type IgM (immunoglobulin) antibody. The detection kit comprises a detection strip, wherein the detection strip comprises a gold conjugate pad and a reaction film, the gold conjugate pad is coated with a colloidal gold labeled mouse-anti-human IgM monoclonal antibody, the reaction film comprises a detection line and a quality control line, the detection line is coated with a recombinant HPVB19 antigen, and the quality control line is coated with a goat-anti-mouse IgG polyclonal antibody. The invention further discloses a preparation method of the kit. The preparation method comprises a reaction film preparation step, a gold conjugate pad preparation step as well as a cutting and assembly step. The kit is simple and convenient to operate, low in cost, good in specificity and high in sensitivity, is suitable for popularization and early-stage diagnosis, can perform single detection and is applicable to epidemiologic investigation and field application.

Nucleic acid oligomers specific for human parvovirus genomic DNA are disclosed. An assay for amplifying and detecting human parvovirus genotypes 1, 2 and 3 nucleic acid in biological specimens is disclosed. Compositions for amplifying and detecting the presence of human parvovirus genotypes 1, 2 and 3 genomic DNA in human biological specimens are disclosed.

A method for detection of human parvovirus B19, comprising the steps of: (1) bringing a sample into contact with fixed P-antigen positive red cells in a medium at pH 5.6±0.6; and (2) determining whether or not hemagglutination occurs; and a reagent for detecting human parvovirus B19, wherein the reagent comprises fixed P-antigen positive red cells.

The invention discloses a kit for detecting a human parvovirus B19. The kit contains a primer pair with a nucleotide sequence as shown in SEQ ID NO:1-2, and the primer pair amplifies a gene from the human parvovirus B19. The kit disclosed by the invention can be used for amplifying the gene segment of the human parvovirus B19, has the advantages of high specificity, high sensitivity and good repeatability, is used for fast detecting a blood product and has good application prospect.

A novel receptor for human parvovirus B19, other than P antigen, as well as a reagent for measuring human parvovirus B19, a reagent for adsorbing human parvovirus B19, and an agent for suppressing infection, which utilize the receptor for human parvovirus B19 is disclosed. It was discovered that Ku 80 protein is a receptor for human parvovirus B19. Thus, a receptor for human parvovirus B19 consisting essentially of Ku80 was provided. The agent for binding human parvovirus B19 comprises Ku80. The agent for suppressing infection by human parvovirus B19 comprises a substance which inhibits the binding between Ku80 and human parvovirus B19 or a binding fragment thereof.

The present invention relates to the discovery of a new human parvovirus, methods of detecting the parvovirus and diagnosing parvovirus infection, methods of treating or preventing parvovirus infection, and methods for identifying anti-parvoviral compounds.

The invention provides a recombinant human parvovirus B19 protein and application of the recombinant human parvovirus B19 protein in preparation of a detection kit. Compared with similar kits on market, the detection kit prepared by adopting the recombinant human parvovirus B19 has the advantages of strong specificity and high sensitivity, and need of clinical diagnosis pm human parvovirus B19 infection is met.

A kit for detecting human Parvoviridae B19, the kit comprising a primer pair shown by nucleotide sequence such as SEQ ID NO: 1-2, and amplifying the genes from human Parvoviridae B19. The kit can amplify gene fragments of human Parvoviridae B19 and has strong specificity, high sensitivity, good repeatability, and is used for quick testing of blood products and has good application prospects.

Nucleic acid oligomers specific for human parvovirus genomic DNA are disclosed. An assay for amplifying and detecting human parvovirus genotypes 1, 2 and 3 nucleic acid in biological specimens is disclosed. Compositions for amplifying and detecting the presence of human parvovirus genotypes 1, 2 and 3 genomic DNA in human biological specimens are disclosed.

Nucleic acid molecules derived from sequences of novel human parvovirus B19 variant genomes are provided. Also provided are assays and kits comprising the nucleic acid molecules.

The invention provides a kit and a method for detecting human parvovirus B19, which integrate sample treatment, nucleic acid extraction and multiple isothermal amplification. The kit comprises an isothermal amplification detection reagent containing primers of which the nucleotide sequences are SEQ ID NO: 3-10, a reagent for nucleic acid separation / purification, a negative control and a positive control, the isothermal amplification detection reagent and the reagent for nucleic acid separation / purification are converted into freeze-dried powder by adopting a special freeze-drying process. According to the method provided by the invention, the DNA of the sample to be detected is rapidly obtained through the PCR reaction tube containing the micro separation tube, and the human parvovirus B19 is accurately detected by adopting a multiple constant-temperature probe method. According to the kit and the method provided by the invention, one or more defects of the existing isothermal amplification method are overcome, and particularly, 'sample feeding and result discharging 'in a real sense is realized; the detection sensitivity is remarkably improved while the storage and transportation cost is reduced, POCT detection is convenient to realize, and the kit can be widely applied to hospital outpatient emergency treatment and primary medical institutions.

A method for detecting human parvovirus / erythrovirus antigen in a sample comprises contacting a buffer having a pH in the range 3.0 to 4.0, suitably a citrate / trisodium citrate buffer, with the sample followed by the measurement of the antigen. The measurement of the antigen can be by virus capture enzyme immunoassay. The method is a good indicator of recent infection and can be used in the screening of individual plasma units or pools from which blood products are extracted.

The invention provides a primer, probe, reagent kit and method for detecting human parvovirus B19 based on a real-time fluorescence PCR technique. The primer and the probe consist of two groups: a first group including a template 1 as shown in SEQID NO:7, a forward primer 1 as shown in SEQID NO:1, a backward primer 1 as shown in SEQID NO:2 and a probe 1 as shown in SEQID NO:5, and a second group including a template 2 as shown in SEQID NO:8, a forward primer 2 as shown in SEQID NO:3, a backward primer 2 as shown in SEQID NO:4 and a probe 2 as shown in SEQID NO:6. In accordance with the human parvovirus B19 of all different types, corresponding forward and backward primers, corresponding probes and a reaction procedure are designed, a real-time quantitative PCR method having the advantagesof being good in specifity, good in sensitivity, high in spreadability of variants and the like is established, and the detection method is suitable for heavy and complicated detection of mass production of biological products.

The invention belongs to the field of application of biologic techniques, and particularly relates to a human parvovirus and humanbocavirus double real-time fluorescent PCR detection primer, probe andreagent kit, and a detection method. The effects of quantitative detection, high sensitivity, high specificity and false negative prevention are achieved, a complete solution is provided for quick quantitative detection of human parvovirus B19V and humanbocavirus in clinical samples, and quick accurate quantitative detection of the human parvovirus B19V and the humanbocavirus can be realized.

The invention relates to a human parvovirus gene engineering artificial expression antigen. A method for preparing the antigen comprises the steps that part of gene sequences of a human parvovirus VP2 protein antigen are artificially synthesized, a prokaryotic expression vector is constructed, the human parvovirus VP2 protein antigen is expressed by escherichia coli, an inclusion body is renatured by adopting a dialysis method, a gradient dilution method and a gel chromatography method, and the recombinant human parvovirus VP2 protein antigen with a three-dimensional structure and immunocompetence is obtained. A rapid detection method for a human parvovirus antibody comprises the step of applying the human parvovirus VP2 protein antigen. A rapid detection kit for detecting the human parvovirus antibody comprises the human parvovirus VP2 protein antigen and can be directly applied to whole blood detection. The kit comprises a rheumatoid factor disposal pad and can remove rheumatoid factors in a sample and directly detect IgM in the sample. The human parvovirus antigen has the high specificity. The method for preparing the antigen, the method for rapidly determining the human parvovirus antibody and the kit for rapidly determining the human parvovirus antibody are provided.

Provided herein are sequences of the genomes and encoded proteins of a new human parvovirus, Humink parvovirus, and variants thereof. Also provided are methods of detecting the Humink parvovirus and diagnosing Humink parvovirus infection, methods of treating or preventing Humink parvovirus infection, and methods for identifying anti-Humink parvovirus compounds.