Preparation method of diagnostic kit for pig hepatitis E virus ELISA
A diagnostic kit and porcine hepatitis E technology, applied in the field of immunology, to achieve strong specificity, qualified stability, and good repeatability
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[0021] 1. Computer screening of porcine hepatitis E virus epitopes and synthesis of peptides
[0022] The computer software is used to screen the recognition epitopes from the corresponding proteins of the open reading frames ORF1, ORF2 and ORF3 of the whole genome of porcine hepatitis E virus. Epitope recognition is mainly based on the hydrophilicity, surface degree, flexibility of protein or polypeptide amino acid sequence, Chou-Fasman's helix, sheet and turn, Robson-Garnier's secondary structure and C- and N-terminus to predict Antigen comprehensive data (Antigenic Index, AI). Using existing software, such as: GCG (Madison, Wisconsin, USA) and DNAStar (Madison, Wisconsin, USA) to calculate AI and auxiliary recognition antigen regions from ORF1, ORF2 and ORF3 proteins of pig HEV. According to the amino acid sequences obtained from the known proteins of ORF1, ORF2 and ORF3 of porcine HEV, computer programs were used to estimate their antigenic regions (AI) (generally 10 to 1...
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