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method for structuring hepatitis e infection model

A technology of hepatitis E and construction method, which is applied in the field of construction of hepatitis E infection model, can solve problems such as inconvenient feeding, long breeding cycle, and complicated test operation, and achieve simple and easy test operation, convenient feeding and management, and experimental The effect of easy operation

Inactive Publication Date: 2011-03-30
CHINA AGRI UNIV
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Problems solved by technology

[0003] Currently used non-human primate infection models are expensive, limited sources, high experimental requirements, long breeding cycle, complicated test operations, and inconvenient breeding, which greatly restrict their application

Method used

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Embodiment 1

[0016] Embodiment 1, the construction of hepatitis E infection model and its effect verification

[0017] 1. Construction of hepatitis E infection model

[0018] (1) Experimental gerbils (purchased from the Department of Experimental Animal Science, Capital Medical University): use SPF grade or clean grade, 50-80 g long-clawed gerbils. After antibody detection, HEV IgG-negative mice were selected, and 28 mice were selected and divided into two groups, 14 mice in each group, and raised separately in the isolation system.

[0019] (2) challenge: select HEV gene type 4 virus (the sequence of its genome is the sequence shown in FJ409465 numbered in GENBANK) copy number is at 10 5 The above pig liver disease materials were used to prepare liver suspension, and each long-clawed gerbil was orally administered with 10 5 -10 8 copies of virus (genome dose), at 10 6 1 virus copy is the optimal infection dose, in the present embodiment, HEV content is 10 in every milliliter suspensio...

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Abstract

The invention discloses a method for structuring a hepatitis E infection model, which comprises the following steps of: infecting Meriones unguiculatus with hepatitis E viruses to obtain the hepatitis E infected model. The infection method is oral administration. The method of the invention has low price, adopts the Meriones unguiculatus infection model which is convenient to feed and test, and avoids the defects of high price, limited sources, high experiment requirement condition, long propagation period and the like of non-human primates used at present. The invention provides the ideal infection model for the study of HEV (hepatitis E virus) pathogenesis, vaccine development, interspecies transmission mechanism and the like.

Description

technical field [0001] The invention relates to a method for constructing a hepatitis E infection model, in particular to a method for constructing a hepatitis E gerbil infection model. Background technique [0002] So far, in the research of hepatitis E, there is still a lack of cell lines for successfully culturing hepatitis E virus (HEV), and HEV research mainly relies on experimental animals. The application of primate models such as rhesus monkeys and chimpanzees enables HEV research to focus on virus replication sites, shedding and viremia duration, antibody growth and decline, genotype, subtype pathogenicity differences, and cross-species transmission mechanisms, etc. Great progress has been made. However, primates have disadvantages such as high price, limited sources, high requirements for experimental conditions, and difficulty in using them in large quantities. For a long time, scientists from various countries have tried to establish a rodent infection model, b...

Claims

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Application Information

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IPC IPC(8): A61K35/76A61P31/14A61P1/16A61K49/00
Inventor 佘锐萍李文贵李睿文毛晶晶尹君尤华夏抗抗丁叶
Owner CHINA AGRI UNIV
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