The invention belongs to the technical field of medicine, in particular to methods for preparing and detecting a compound dyers woad leaf suppository. The suppository is prepared from the following raw materials in parts by weight: 460-500 g of a Chinese medicinal extract prepared from dyers woad leaves, honeysuckle flower, notopterygium root, rhubarb and bistort rhizome in a ratio of 4:2:1:1:1, and containing chlorogenic acid not less than 8 mg / g and emodin chrysophanol not less than 0.9 mg / g, 900-1000 g of fatty acid glyceride matrix, 40-60 mL of span 80 and 80-120 ml of volatile oil. TLC isused to identify indirubin, chlorogenic acid, rhubarb and Notopterygium root, and HPLC is used to determine emodin and chrysophanol in Compound Isatis Suppository. The preparation method is simple and feasible, and meets the needs of industrial production; The detection method is scientific, specific, simple, sensitive and stable, which improves the effective control of drug quality and guarantees the quality.