68 results about "Filtration diaphragm" patented technology

The invention discloses a method and a device for acquiring the streaming potential of a nanofiltration film and relates to an analyzing method for separating the streaming potential from the measured transmembrane potential. The invention measures the transmembrane potential, pressure difference, flux and rejection rate, implements nonlinear least squares fitting on the rejection rate and the flux according to the Spiegler-Kedem equation, thus acquiring the reflectance, furthermore, pressure difference of the hierarchy is treated as the tag pressure difference when the rejection rate approaches the reflectance, and the invention implements linear fitting on the measured pressure difference which exceeds the tag pressure difference and the corresponding transmembrane potential, the size of the slope coefficient is taken as the streaming potential. The measurement device comprises a thermostatic bath, a liquid storage bath, a constant flow pump and a membranous device; wherein, the liquid storage bath is positioned inside the thermostatic bath, the constant flow pump, the liquid storage bath and the membranous device are connected with each other through nonmetallic catheters, a pressure gauge, a potentiometer and a flowmeter are connected at two sides of the membranous device. The method and the device provided by the invention can successfully separate the streaming potential from the transmembrane potential and provide data support for representing the electrical property and the separability of the nanofiltration film.

The present invention discloses a blood type detection method based on a membrane structure. According to the method, a filtration membrane allowing a single red blood cell to pass through is selected as a core material, blood type antibodies are bound to the filtration membrane, a water absorption material is surrounding the periphery of the filtration membrane, red blood cells with a certain concentration are added on the filtration membrane surface in a dropwise manner during detecting, rinsing is performed with a rinsing liquid, the red blood cells with no antigen-antibody binding exude along with the rinsing liquid, the red blood cells with the antigen-antibody binding are remained on the membrane surface so as to form the visible red clumps, and an operator can judge the result according to whether the red blood cells form the visible red clumps on the filtration membrane so as to determine the blood type of red blood cells. The method of the present invention has beneficial effects of short detection time and objective result.

The invention relates to a biological agent for treating habitual abortion in the medicine technological field, which comprises the following steps: extracting peripheral blood of healthy adult men; diluting the blood to 1. 5 times through glass fibers from the ball and Hanks liquid to separate lymphocyte to collect single core cells; washing by Hanks liquid to culture and flow and count with absolute culture liquid of RPMI-1640; adjusting the cell concentration to 2X106/ml to add phaescolosaxin to culture at 37 Deg. C for 72hours; collecting supernatant to produce T cells through centrifuging of centrifugal ultrafiltration and cane sugar density gradient centrifugation; filtering by sterile filter membrane to be split charging to produce completed product at 80 Deg. C below zero. The invention evokes immune tolerance of body effectively and overcomes the more faults of evoking immune tolerance through cell adoptive immunotherapy to make easier clinical application, which can be produced in a large industrialization scale to get more stabilizing mass control and higher controllability for treating the habitual abortion with good curative effect.

The invention relates to clinical single-tube fluorine 18 multifunctional module equipment and a radiopharmaceutical synthesis process. The clinical single-tube fluorine 18 multifunctional module equipment comprises a fluorine-18 ioncapturing and releasingmodule, a high performance liquid chromatography column purification module and a product collection module, wherein the high performance liquidchromatography column purification module adopts liquid for intravenous injection as an HPLC mobile phase; a QMA column of the fluorine-18 ion capturing and releasing module captures fluorine-18 ionsgenerated from an accelerator, and the leacheate of the QMA column and a prodrug respectively enter a reaction tube for mixing with the HPLC mobile phase; the reaction tube is connected with an HPLCseparation column of the high performance liquid chromatography column purification module; the HPLC separation column is connected with the product collection module; and a product having been subjected to online dilution and sterile filtration membrane treatment can be used for intravenous injection. By adopting the equipment and the process provided by the invention, solvent conversion used bya common module can be avoided, a target compound can be correctly collected, the pH value and the radioactive concentration of the product can be adjusted on line, and the process is suitable for full-automatic preparation of clinically common positron emission drugs under a GMP condition.

The invention discloses a portable water purifying device which comprises a casing, wherein a hand-operated water pump and a water filter device are mounted in the casing; the hand-operated water pump comprises a rocking handle, a reduction box and a pump body which are sequentially connected, the pump body is provided with a first working cavity and a second working cavity which are mutually spaced and provided with water inlets and water outlets, and a water inlet of the first working cavity is connected with a water pumping pipe extending out of the casing; and the water filter device comprises an ultra-filtration diaphragm filter, an RO (reverse osmosis) diaphragm filter and a water storage box, the water outlet of the first working cavity, a water inlet and a water outlet of the ultra-filtration diaphragm filter, a water inlet and a water outlet of the water storage box, the water inlet and the water outlet of the second working cavity, as well as a water inlet and a water outlet of the RO diaphragm filter are sequentially connected in series and communicated, and the water outlet of the RO diaphragm filter is connected with a water outlet pipe extending out of the casing. The portable water purifying device can pump water at 10-15 m depth underground, the lift can reach a height of 150-200 m, and various types of water can be deeply filtered and treated to meet the standard of purified water for drinking.

The present invention discloses a circulating tumor cell isolation and enrichment device based on a closed loop. The circulating tumor cell isolation and enrichment device based on the closed loop includes a top plate; two ends of the bottom of the top plate are respectively fixedly connected with a left bracket and a right bracket located at the right side of the left bracket; the left side of the right bracket is fixedly provided with a device main body which is of a hollow structure; the bottom of the device main body is provided with a sample outlet where a liquid collecting bottle is disposed, and a filter membrane is further disposed between a top bottleneck of the liquid collecting bottle and the sample outlet; the inside of the device main body is movably provided with an oscillation plate; and the top of the oscillation plate is fixedly provided with a lifting bar and a toothed bar located at the right side of the lifting bar. In the circulating tumor cell isolation and enrichment device based on the closed loop, the activity of a sample liquid in the device main body may be accelerated, the disadvantage that an original liquid is relatively still and relatively low in flowability and activity is changed, and the isolation of the sample liquid and the filter membrane in a cell enrichment device may be further accelerated, so that the tumor cell isolation and enrichmentefficiencies are greatly increased, and the use demands of people are met.

The present invention relates to a method for filtering blood to produce plasma or serum, comprising the steps of: a. providing a blood filter comprising a filter membrane (6) having opposite first and second sides and a receiving compartment (2) defining a hollow space; and b. inserting a blood sample (1) into said receiving compartment (2), wherein the volume of said hollow space is 3 to 20 times larger than the volume of said blood sample (1), thereby increasing the gas pressure within said receiving compartment so that said blood sample is filtered by said filter membrane (6), and wherein said plasma or serum comprised in said blood sample is forced through said filter membrane. The invention also relates to a method for filtering blood to produce plasma or serum, comprising the steps of: a. providing a blood filter comprising a filter membrane (6) having opposite first and second sides and a receiving compartment (2'') having a first volume; b. inserting a blood sample (1) and a gas (2') into a syringe, the blood sample (1) occupying a second volume and the gas (2') occupying a third volume in the syringe; c. connecting said syringe to said blood filter to be in fluid communication with each other; and d. increasing the pressure within said syringe until the blood sample (1) is received by said receiving compartment (2''), so that said blood sample (1) is filtered by said filter membrane (6), wherein said plasma or serum comprised in said blood sample is forced through said filter membrane (6); wherein the sum of first and third volume is 3 to 20 times larger than the second volume. The present invention also relates to a filter membrane (6) having opposite first and second sides; a receiving compartment (2) defining a hollow space for receiving a blood sample to be filtered, said receiving compartment having at least one opening covered with said filter membrane (6), wherein said first side is facing the receiving compartment (2), and wherein the hollow space of said receiving compartment has a volume being 3 to 20 times larger than the volume of the blood sample to be filtered; and a sampling compartment (10) being arranged on said second side of said filter membrane (6).

The invention discloses a safe water surface treatment device for oil leakage. The safe water surface treatment device comprises a box body, a water inlet pipe, a pressing plate, a drain pipe and an igniter, wherein the water inlet pipe is arranged at the bottom end of the box body, a pump and a flow meter are arranged on the water inlet pipe, grids are arranged in the water inlet pipe, the igniter is arranged on the inner wall of the box body, an air bag is arranged at the bottom end of the box body, an oxygen filling tank is mounted on the outer wall of the box body, a breather pipe is arranged on the oxygen filling tank, the breather pipe is provided with two outlet ends, one outlet end of the breather pipe is connected with an air valve of the air bag, the other outlet end of the breather pipe is provided with a spray row, the spray row is arranged in the box body, the side wall of the box body is communicated with an air collector through a suction pipe, a gas one-way valve is mounted on the suction pipe, a water filtration diaphragm plate is arranged in the box body, a telescopic rod is arranged on one side of the water filtration diaphragm plate, and the pressing plate is arranged at the bottom end of the telescopic rod. The safe water surface treatment device reduces oil contamination, facilitates environmental protection, and is simple in structure, convenient to use,and beneficial to popularization.

The invention relates to the technical field of engineered exosomes, and discloses a method for increasing the content of exosomes released by cells, which comprises the following steps: 1) treating a cancer cell line; 2) filtering the treated cancer cell supernatant with a 0.45 [mu] m filter membrane, and collecting the filtered liquid; (3) centrifuging 120000g of the solution for 90 minutes, and extracting the exosome; 4) collecting the exosome; the cancer cell line treatment comprises the following steps: 1) replacing a fresh culture medium for cancer cells; 2) irradiating cancer cells with UVB for 1 hour, and paying attention to the state of liver cancer cells at any moment; 3) culturing the cancer cells in an incubator for 2 hours; 4) placing the cancer cells in an anoxic device for 1 h; and 5) collecting the supernatant of the cancer cells after 24 hours. The exosome release amount can be increased under the condition that cell genetic materials are not changed, the method is suitable for various cell lines, compared with an ultrasonic method, an LED lamp irradiation method, a special culture medium treatment method and other methods, the method is simpler, and the exosome release amount of the various cell lines can be better increased.

The invention discloses a full-automatic sheep blood PBMC (peripheral blood mononuclear cell) separation system and method. The full-automatic sheep blood PBMC separation system comprises an input module, a separation module, an output module, a pipeline module and a power module, wherein the pipeline module comprises an input pipe, a peristaltic pipe and an output pipe; the head end of the input pipe is provided with a filter membrane structure, and the tail end of the input pipe and the head end of the output pipe both communicate with the head end of the peristaltic pipe; and the input end and the output end of the separation module both communicate with the tail end of the peristaltic pipe, and the power module is arranged on the peristaltic pipe and used for driving liquid in the peristaltic pipe to flow. The separation process is full-automatic, the consistency of the production environment, the raw materials and the production process can ensure that each batch of cells are prepared under the same standard condition, and the stability and uniformity of the cell quality and the repeatability of experimental results are ensured. The problem that the peripheral blood mononuclear cells need to be extracted and prepared on a large scale in scientific research and new drug research and development animal experiments is solved, traditional manual operation is changed into automatic operation through intelligent equipment, efficiency is improved, and time of scientific research personnel is saved.

The invention relates to the technical field of medical treatment, in particular to a pretreatment tube and a pretreatment method for a liquid-based cell sample.The pretreatment pipe comprises a pipe body; a pair of slots which are formed in the inner wall of the pipe body; an inserting plate, the width of the inserting plate is matched with the spacing distance of the pair of inserting grooves, and the inserting plate is used for being embedded into the pair of inserting grooves so as to divide the pipe body into a first cavity and a second cavity which are closed with each other; a filter membrane is arranged on the insertion plate and is provided with a plurality of filter holes, and the size of the filter holes is smaller than that of the cells to be treated. According to the technical scheme, the pretreatment tube has the beneficial effects that the insertion plate is arranged in the tube body of the pretreatment tube to divide the tube body into the first cavity and the second cavity, and the insertion plate is provided with the filter membrane with the filter holes, so that the tube body is not only used for storing cell samples, but also can realize other reaction functions such as dilution, enrichment and dyeing in the tube body.

The invention discloses a filtering centrifuging tube type lymphocyte culture tube and a culture method. The culture tube comprises a centrifuging tube, a filter and a tube cover, wherein an upper part of the centrifuging tube is a cylindrical tube, the filter is a detachable cylindrical tube, the diameter of the bottom of the filter is smaller than that of a tube body of the filter, the bottom ofthe filter is a meshed bottom face, a filter membrane and a pressing ring are sequentially arranged above the bottom of the filter, an external hanging edge is arranged at a tube orifice of the filter, a circular hole is formed in the center of the tube cover, and a rubber plug is arranged inside the tube cover. According to the lymphocyte culture method, lymphocytes are cultured by using the culture tube. The culture tube is simple in structure and ingenious in design and integrates the functions of culturing, centrifuging and cell filtering; and by using the innovative centrifugal culture tube with the filter, convenience is provided for human peripheral blood chromosome karyotype analysis, operating steps are greatly simplified, the experimental time is shortened, and meanwhile, a stable experimental result can be obtained.

The invention provides a cross-flow filtration system for enriching circulating tumor cells. The system comprises: a tumor cell sap-to-be-filtered volumetric flask (1); three peristaltic pumps which are a first peristaltic pump (2-1), a second peristaltic pump (2-2) and a third peristaltic pump (2-3) respectively; a centrifugal tube (3); a filtering chip (7); and a cross-flow filtered tumor cell sap volumetric flask (5). A process represented by figure 1 is a new method provided on the basis of filter membrane filtration and a micro-fluidic fluid principle. The cross-flow filtration is characterized in that the flow direction of a water phase is parallel to the surface of the filter membrane to form a large pressure on the surface of the filter membrane, so cells enter filter holes, and are filtered and screened, and the activity of the cells is not affected after the filtration in order to achieve the efficient enrichment effect. The cross-flow filtration system for enriching circulating tumor cells reaches the efficient enrichment effect. The system has the advantages of simplicity in sample treatment, high recovery rate of the sample, simplicity in operation, convenience in use,facilitation of promotion, good clinic application prospect and great social benefit.

Experimental microfiltration membrane equipment for concentrating a Dunaliella salina liquid is used for concentrating a Dunaliella salina liquid. The equipment comprises a microfiltration membrane, awater inlet pipe, a first ball valve, a pressure vacuum gauge, a diaphragm booster pump, a second ball valve, a third ball valve, a flowmeter and a water outlet pipe connected in sequence; the diaphragm booster pump is powered and the equipment is controlled to start and stop through the arrangement of a time relay and a power adapter. The time relay, the pressure vacuum gauge, the diaphragm booster pump, the power adapter and the second ball valve are fixed inside a distribution box, and the first ball valve, the third ball valve and the flowmeter are respectively fixed on different outer side walls of the distribution box. The invention has the beneficial effects as follows: the structure is simple; by setting the concentration time and controlling pre-pumping pressure of the diaphragmbooster pump, the pre-pumping pressure of the diaphragm booster pump is controlled to be 0.01 MPa and below; and after membrane filtration for concentration, the Dunaliella salina activity is kept good, and after late culture, various physiological indexes are not much different from physiological indexes of the original control group, and the concentration effect is ideal.

The invention discloses gas-liquid separation equipment facilitating gas concentration testing. The gas-liquid separation equipment comprises an upper casing, a lower casing and a gas-liquid filtration diaphragm which constitute a gas accommodating cavity, a transition cavity is formed between the lower casing and the gas-liquid filtration diaphragm, a liquid inlet pipe and a liquid outlet pipe which are communicated with the transition cavity are arranged on the side wall of the lower casing, a control assembly is arranged on the liquid inlet pipe, the control assembly comprises a pressure limiting assembly arranged on a pipeline between a liquid inlet and a liquid inlet as well as a pressure relief assembly arranged on a channel connecting the liquid outlet and a liquid drain port in the pressure limiting assembly. Spiral isolation plates wind around the outer side wall of the gas-liquid filtration diaphragm and divide the transition cavity to form spiral channels communicated withthe liquid inlet pipe and the liquid outlet pipe. The gas-liquid separation equipment can avoid damage to inner assemblies and is convenient to operate and high in separation efficiency when separating gas in a liquid, and thus, the detection efficiency of gas in the liquid can be improved.