Cell transfer device and cell transfer and pretreatment method

Abstract

The invention relates to the technical field of medical instruments and particularly relates to a cell transfer device. The device comprises a suction head, a three-axis mechanical arm, a main machine, a suction nozzle head and a positive and negative pressure generating device, wherein the suction head is of a hollow structure with openings in the two ends, an annular protrusion is arranged on an opening in a lower end of the suction head, and a filter film is welded to the protrusion; the main machine is arranged on the three-axis mechanical arm, one end of the suction nozzle head is connected with the main machine, the other end of the suction nozzle head is connected with an upper end opening of the suction head, and a through hole is formed in the suction nozzle head; the positive and negative pressure generating device is arranged in the main machine, the positive and negative pressure generating device is communicated with an inner cavity defined by the side wall of the suction head through the through hole, and the positive and negative pressure generating device is used for separating cells from the mixed liquid through the suction head and absorbing the cells on the filter film or blowing the cells from the filter film away from the filter film; and the invention further provides a cell transfer and pretreatment method adopting the cell transfer device. According to the method, cell pretreatment and cell transfer can be carried out at the same time, the operation process is simplified, centrifugal separation treatment is avoided, and staining and slide-making efficiency is effectively improved.

Description

technical field [0001] The invention relates to the technical field of medical devices, in particular to a cell transfer device and a cell transfer and pretreatment method. Background technique [0002] In the prior art, when analyzing cells, nuclei or other organelles, it is necessary to collect the target cells, nuclei or organelles. The commonly used method is density gradient centrifugation, which is to pretreat the cells with the preservative solution after coarse filtration. centrifugal. The current sample pretreatment method is usually to filter the sample into a container, then transfer the filtrate in the container to a centrifuge tube, add gradient centrifugation solution, remove the supernatant after centrifugation and add cell buffer, and then use TIP The mechanism or manual pipette gun transfers the centrifuged cells to the desired container, which is a complicated process and prone to bacterial contamination. [0003] For example, the porous plate filtration ...

AI Technical Summary

Problems solved by technology

In the above technical solution, the operation is complicated, the implementation process has many steps, and it is easy to be contaminated by bacteria during the manual operation process, and the positive pressing block or the guide block is not cleaned when it is reused, which may easily cause positive cells to remain and cause crossover between samples. Pollution

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