Filtering centrifuging tube type lymphocyte culture tube and culture method

A lymphocyte, filtration and centrifugation technology, which is applied in the direction of tissue cell/virus culture device, sterilization method, biochemical equipment and method, etc., can solve the problem that the culture tube does not have a filter device, so as to shorten the experiment time and stabilize the Experimental results, the effect of a simple structure

Pending Publication Date: 2020-01-21
迈健医药科技无锡有限公司
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

This culture tube does

Method used

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  • Filtering centrifuging tube type lymphocyte culture tube and culture method
  • Filtering centrifuging tube type lymphocyte culture tube and culture method
  • Filtering centrifuging tube type lymphocyte culture tube and culture method

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Experimental program
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Embodiment 1

[0039] as attached Figure 1-3 Shown: the present invention aims at the characteristics of human peripheral blood lymphocyte culture and chromosome preparation, and develops a lymphocyte culture medium with stable pH, which can be used for in vitro culture of peripheral blood lymphocyte culture medium, suitable for airtight culture, and can be used in Cultivate in a water-proof constant temperature incubator or a water bath. Centrifugal culture tubes can simplify the post-production process and facilitate operation.

[0040] A filter centrifuge tube type lymphocyte culture tube, comprising a centrifuge tube 1, a filter 2, and a tube cover 3, the upper part of the centrifuge tube 1 is a cylindrical tube, the lower part is conical, and the inside of the tube cover 3 is provided with threads , The mouth of the centrifuge tube 1 is provided with threads, and the threads of the tube cover 3 are matched with the threads of the centrifuge tube 1 .

[0041] The filter 2 is a detacha...

Embodiment 2

[0047] as attached Figure 1-6 Shown:

[0048] A method for culturing lymphocytes, using the above-mentioned culture tube.

[0049] After disinfection with sterile cotton pads, 2 ml of peripheral venous blood was collected using a heparin anticoagulant tube and a blood collection needle, and mixed by inversion. Get a tube of peripheral blood lymphocyte culture medium among the present invention (as attached Figure 4 (shown) placed at room temperature to thaw, in the ultra-clean workbench, draw about 0.3 ml of peripheral blood with a 2 ml disposable sterile syringe, insert the needle into the rubber stopper of centrifuge tube 1, inject the peripheral blood into the culture medium, pull out Needle (discard), mix gently. Place the culture tube on the medium rack and place it in a 37°C constant temperature incubator for 72 hours. Shake the culture tube once a day to disperse the cells. Before terminating the culture for 2 hours, draw 0.1 ml with a 2 ml syringe (do not discard ...

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Abstract

The invention discloses a filtering centrifuging tube type lymphocyte culture tube and a culture method. The culture tube comprises a centrifuging tube, a filter and a tube cover, wherein an upper part of the centrifuging tube is a cylindrical tube, the filter is a detachable cylindrical tube, the diameter of the bottom of the filter is smaller than that of a tube body of the filter, the bottom ofthe filter is a meshed bottom face, a filter membrane and a pressing ring are sequentially arranged above the bottom of the filter, an external hanging edge is arranged at a tube orifice of the filter, a circular hole is formed in the center of the tube cover, and a rubber plug is arranged inside the tube cover. According to the lymphocyte culture method, lymphocytes are cultured by using the culture tube. The culture tube is simple in structure and ingenious in design and integrates the functions of culturing, centrifuging and cell filtering; and by using the innovative centrifugal culture tube with the filter, convenience is provided for human peripheral blood chromosome karyotype analysis, operating steps are greatly simplified, the experimental time is shortened, and meanwhile, a stable experimental result can be obtained.

Description

technical field [0001] The invention belongs to the field of cell culture containers, in particular to a filter centrifugal tube type lymphocyte culture tube and a culture method. Background technique [0002] In 1960, Nowell and Morhead verified that phytohaemagglutinin (PHA), which agglutinates red blood cells and separates white blood cells, is a mitotic stimulator of lymphocytes in humans and other animals. Under the action of PHA, the lymphocytes originally in the G1 phase transform into lymphoblasts, and then undergo mitosis. In this way, after short-term culture, treated with colchicine or its derivative colcemid, after hypotonicity and fixation, a large number of cells in mitosis can be obtained, because colchicine (or colchicamide) can interfere with microtubule Assembling and inhibiting the formation of spindle filaments, cell division cannot smoothly enter anaphase and stagnate in metaphase, so that a large number of metaphase phases that are most suitable for ch...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/24C12M1/12
CPCC12M23/06C12M29/04
Inventor 冷晓燕段颖王强段云飞常菁虞强
Owner 迈健医药科技无锡有限公司
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