The invention relates to a fracture model preparation device for animal experiments. The device comprises a base, a supporting plate, two supporting side columns, a force application mechanism and two supporting sheets, the two supporting side columns are symmetrically fixed to the base, and the supporting plate is horizontally arranged between the two side columns; the force application mechanism is composed of two spring sleeving rods which are vertically fixed onto the supporting plate, two springs of same specification which are arranged on the two spring sleeving rods in a sleeving mode respectively, a weight supporting plate and a force application rod, the force application rod vertically penetrates through the weight supporting plate and is fixed to the weight supporting plate, and the weight supporting plate is arranged on the two spring sleeving rods in a sleeving mode and supported by the springs; the axis of the force application rod and the axes of the two spring sleeving rods are in a same vertical plane, the distances between the force application rod and the two spring sleeving rods are identical, the lower end of the force application rod movably and vertically penetrates through a penetrating hole which is correspondingly formed in the supporting plate, and the two supporting sheets are relatively movably arranged on the base. According to the fracture model preparation device for the animal experiments, production of fracture sites of relatively identical positions is achieved, and the soft tissue wound is reduced to a certain extent.