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51results about How to "High interference efficiency" patented technology
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Radar jamming method and device as well as frequency phased array jammer
InactiveCN107037410AAddressing Range and Range ResolutionStrong interference abilityWave based measurement systemsPhysicsVIT signals
The invention relates to a radar jamming method and device as well as a frequency phased array jammer. In the frequency phased array jammer, a digital radio frequency memory is used as a front-end basic component to store the received enemy detection signals, forwarding jamming or smart jamming is used flexibly, and finally, an frequency phased array antenna generates jamming signals to produce false targets more than conventional jamming ones, so that detection and tracking of an enemy radar on a moving target are effectively destroyed. The method has the innovation that the novel frequency phased array is applied, the frequency phased array antenna transits jamming signals, and the jamming signals transmitted between the array elements are different in frequency, so that on the one hand, the power of the jamming signals is strengthened in an array form, and on the other hand, the transmitted jamming signals are different in frequency and thus seriously puzzle the detection of the target of the enemy radar in frequency.
Owner:UNIV OF ELECTRONIC SCI & TECH OF CHINA
RNA (Ribonucleic Acid) interference vector and application thereof
ActiveCN102191262APromote cloningAvoid restrictionsVector-based foreign material introductionAngiosperms/flowering plantsDicerNucleotide
The invention discloses an RNA (Ribonucleic Acid) interference vector and application thereof. The RNA interference vector provided by the invention comprises FAD2 (fatty acid desaturase 2) gene intron and an upstream polyclone site and a downstream polyclone site on two sides of the FAD2 gene intron, wherein the FAD2 gene intron is the 60th-1190th site nucleotide from the 5' terminal of the sequence 1 in a sequence table. Proven by experiments of the invention, the invention provides an RNA interference vector suitable for plants; shRNA (short hairpin RNA) formed by expression of the RNA interference vector in a receptor is diced by Dicer into siRNA (small interfering RNA); and the siRNA can specifically inhibit the expression of a target gene, thereby achieving the aims of controlling the plant height of paddy and studying the CYP90D2 / D2 gene function.
Owner:INST OF CROP SCI CHINESE ACAD OF AGRI SCI
Fundus observation device
InactiveCN100998495AHigh interference efficiencyHigh precisionUsing optical meansOthalmoscopesOptical pathOphthalmology
A fundus observation device which can simultaneously capture both surface images and tomographic images of the fundus oculi is provided. The fundus observation device (1) has a fundus camera unit (1A), an OCT unit (150), and an arithmetic and control unit (200). The fundus camera unit (1A) has an illuminating optical system (100) and an imaging optical system (120). The arithmetic and control unit (200) forms the surface image of fundus oculi Ef based on signals from fundus camera unit (1A). The OCT unit (150) divides low coherence light LO into the signal light LS and the reference light LR, and detects the interference light LC that can be obtained from the signal light LS passing through fundus oculi Ef and the reference light LR passing through reference mirror (174). The arithmetic and control unit (200) forms tomographic images of fundus oculi Ef based on these detecting results. A Dichroic mirror (134) combines the optical path of the signal light LS toward fundus oculi Ef into the optical path for imaging of the imaging optical system (120), and separates the optical path of the signal light LS towards fundus oculi Ef from the optical path for imaging.
Owner:KK TOPCON
Method of breeding non-flying Harmonia axyridis
InactiveCN108651388AImprove efficiency andReduce economic costsVector-based foreign material introductionAnimal husbandrySurface-active agentsBiology
The invention discloses a method for breeding non-flying Harmonia axyridis. The method uses gene interference methods to interfere with expressions of vestigial or ultrabithorax, key genes of the wingdevelopment of the Harmonia axyridis, to breed the non-flying Harmonia axyridis. The breeding of the non-flying Harmonia axyridis includes three specific methods: injecting dsRNA, feeding dsRNA / nano-carriers, or soaking the body surface of the Harmonia axyridis in dsRNA / nano-carriers / surface active agents, or spraying or dripping the dsRNA / nano-carriers / surface active agents onto the body surfaceof the Harmonia axyridis. The method breeds the non-flying Harmonia axyridis by three potential gene interference methods, and the three potential gene interference methods are highly-efficient and stable and capable of thoroughly solving a problem of difficult colonization of artificially released natural enemies Harmonia axyridis in the control area, and has important theoretical and practicalsignificance. By applying the feeding method and the body wall penetration method to gene interference, the current situation of time-consuming and labor-consuming traditional methods is completely changed, efficiency and success rates are greatly improved, and costs and operation difficulty are reduced. The method for breeding the non-flying Harmonia axyridis has obvious social and economic benefits and no potential ecological risk.
Owner:CHINA AGRI UNIV
Energy-saving wireless local area network (WLAN) all-channel shielding method
ActiveCN102098774AHigh interference efficiencyReduce throughputEnergy efficient ICTPower managementWireless securityLocal area network
The invention relates to an energy-saving wireless local area network (WLAN) all-channel shielding method, and belongs to the technical field of wireless security. The method comprises the following steps of: starting a shielding device to scan channels of the WLAN, and recording the maximum value of a received signal strength indicator (RSSI) on each working channel; calculating the wireless propagation loss (PL) from the shielding device end to the communication equipment end with the maximum RSSI on each channel; determining the minimum transmitting power Yi,j at which the shielding deviceworking on an i channel makes the throughput of a j channel reduced to zero; finding out the maximum value of Yi,j when the shielding device works in different channels and finding out the minimum value Yk, wherein the corresponding channel k is the optimal working channel of the shielding device; and setting the working channel of the shielding device as k, and transmitting a shielding signal atYk power. By the method, all channels can be interfered at the same time, the channel throughput is reduced at the same time, the problem that an interference signal cannot be transmitted when the original channel throughput is raised and skipped due to channel skip is solved, and interference efficiency is improved.
Owner:TIANJIN UNIV
Inter-network interference coordination method
InactiveUS20170290023A1Effectively solves interference coordinationHigh interference efficiencyDistributed allocationNetwork planningSpectrum poolingFrequency spectrum
Disclosed is a network interference coordination method in a co-primary spectrum sharing scenario. The method comprises the following steps: a first network and a second network negotiate an inter-network shared band, the shared band being in a shared spectrum pool; the first network evaluates inter-network interference; the first network determines whether to perform internal coordination, if internal coordination is performed, the first network performs internal coordination; if internal coordination is not performed, request the second network to perform inter-network interference coordination; the second network determines whether to perform internal coordination, if internal coordination is performed, the second network performs internal coordination; and if internal coordination is not performed, go back to the step of negotiating, by a first network and a second network, an inter-network shared band. The present invention can fairly and properly implement inter-network interference coordination in the co-primary spectrum sharing scenario.
Owner:SHANGHAI RES CENT FOR WIRELESS COMM
Research and development method of siRNA for COVID-19 virus drug therapy
ActiveCN111354420AReduce generalization errorHigh interference efficiencyBiostatisticsSequence analysisMolecular biologyDouble strand
The invention discloses a research and development method of siRNA for COVID-19 virus drug therapy, which comprises: a first part of preliminarily screening potential high-efficiency siRNA based on multiple indexes, which specifically comprises the following steps: step 1, selecting an S gene sequence as a target sequence; step 2, obtaining a corresponding siRNA double strand; step 3, screening asiRNA sequence with a concentration of 36 to 53%; step 4, screening out siRNA with free energy; step 5, defining and calculating an index I; step 6, screening out siRNA of the first 50%; step 7, defining and calculating an index II; step 8, screening out siRNA with the index II being equal to 5; step 9, defining and calculating an index III; step 10, ranking the first 50% of siRNA; step 11, directly taking all the candidate siRNAs selected in the step 10; step 12, specifically targeting a target sequence; step 13, taking the remaining siRNA as the siRNA subjected to preliminary screening; anda second part of performing interference efficiency prediction and optimization by using a machine learning model. The method has the beneficial effect of realizing safe, reliable and high-interference-efficiency siRNA design.
Owner:JILIN UNIV
Construction method of bismall molecule interference RNA carrier and its application
InactiveCN1869237AInhibition of replicationHigh interference efficiencyGenetic material ingredientsDigestive systemPaleontologyVirus
The invention discloses a construction method and application for a double micro-molecule disturbance RNA carrier. It includes the following steps: compounding construction primer of double siRNA producing carrier, PCR expanding of siRNA producing module; cloning and sequencing. The invention has the advantages of simple method to construct siRNA carrier, convenient to operate, and high RNA disturbance efficiency. It could make the gene expression that has the same source sequence of siRNA dumbness and would not influence to other functions of the gene. It has strong specificity, and no poison function. It is a good method to cure virus mixed infection by the double siRNA.
Owner:WUHAN UNIV
RNA (Ribonucleic Acid) interference vector and application thereof
ActiveCN102191262BHigh expressionHigh interference efficiencyVector-based foreign material introductionAngiosperms/flowering plantsDicerNucleotide
The invention discloses an RNA (Ribonucleic Acid) interference vector and application thereof. The RNA interference vector provided by the invention comprises FAD2 (fatty acid desaturase 2) gene intron and an upstream polyclone site and a downstream polyclone site on two sides of the FAD2 gene intron, wherein the FAD2 gene intron is the 60th-1190th site nucleotide from the 5' terminal of the sequence 1 in a sequence table. Proven by experiments of the invention, the invention provides an RNA interference vector suitable for plants; shRNA (short hairpin RNA) formed by expression of the RNA interference vector in a receptor is diced by Dicer into siRNA (small interfering RNA); and the siRNA can specifically inhibit the expression of a target gene, thereby achieving the aims of controlling the plant height of paddy and studying the CYP90D2 / D2 gene function.
Owner:INST OF CROP SCI CHINESE ACAD OF AGRI SCI
Targeting liposome for interfering expression of Claudin3 gene
InactiveCN104548130AHigh interference efficiencyImprove anti-tumor effectGenetic material ingredientsPharmaceutical non-active ingredientsGene expressionFolic acid
The invention relates to a targeting liposome containing a Claudin3 gene interference sequence. The targeting liposome takes the Claudin3 gene interference sequence as an active ingredient, and phospholipid and folic acid coupling lipid as carrier materials. The composition can be used for delivering the Claudin3 gene interference sequence to tumor cells in a targeting manner, and can give play to relatively good antitumor activity by efficiently interfering the expression of a Claudin3 gene.
Owner:SICHUAN UNIV
PDF1 gene for controlling plant pollen fertility, and preparation method and application thereof
ActiveCN101974081AHigh interference efficiencyEfficient control of anther fertilityPlant peptidesGenetic engineeringBiotechnologyNucleotide
The invention discloses a protodermal factor 1 (PDF1) gene for controlling plant pollen fertility, and a preparation method and application thereof. A separated protein is obtained from cabbage, cabbage type rape and turnip type rape respectively, wherein sequences of the proteins are nucleotide sequences shown by SEQ ID No. 1, SEQ ID No. 2 and SEQ ID No. 3 respectively. The nucleotide sequences are coding sequences (CDS) which are cloned by taking cDNA as a template. A separated polypeptide is obtained from the cabbage, the cabbage type rape and the turnip type rape respectively, wherein the sequences of the separated polypeptides are amino acid sequences shown by SEQ ID No. 4, SEQ ID No. 5 and SEQ ID No. 6 respectively. The invention discloses the application of the PDF1 gene for controlling the plant pollen fertility, and relates to isolation and cloning, expression analysis and application of the PDF1 homologous gene for controlling microspore development and anther fertility. ThePDF1 is a programmed cell death (PCD) suppression factor; and the gene has obvious expression advantage in rape anther. By weakening the expression of the PDF1 gene through gene engineering technology, the microspore can be aborted, the fertility of the pollen can be controlled, a male sterile material and a restorer material can be created, and the gene can be used for development research of androgamete and improvement on quality of farm crops.
Owner:INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
Method for improving RNA interfering efficiency of mandibulate insects
InactiveCN105779502AReduce dosageHigh interference efficiencyOther foreign material introduction processesEnzymesArthropod mouthpartsBiology
The invention provides a method for improving RNA interfering efficiency of mandibulate insects, comprising: 1), preparing target gene specific siRNA into a solution of certain concentration using water free of RNA enzyme; 2), processing artificial feed into discs 3-5 mm in thickness and 1-2 cm in diameter, and placing the discs in a 24-pore plate; 3), adding the solution of step 1) to the discs of step 2) so that the solution is absorbed by the feed completely; 4), placing newly hatched larvae of mandibulate insects into the 24-pore plate with the feed discs of step 3), feeding for a period of time, and detecting target gene expression of the insects. By modifying the method of feeding insect siRNA, the method for improving RNA interfering efficiency of mandibulate insects is established; the method is efficient and simple and highly operable, usage of siRNA is saved, interfering effect is significant, and technical support is provided for studying the functionality of genes of mandibulate insects.
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
Vibration-sensing corrugated diaphragm, and optical fiber acceleration sensor and micro-vibration detection system based on vibration-sensing corrugated diaphragm
ActiveCN108663538AImprove micro-vibration sensing sensitivityFacilitated releaseAcceleration measurement using interia forcesSubsonic/sonic/ultrasonic wave measurementComposite filmThin membrane
The invention discloses a vibration-sensing corrugated diaphragm, and an optical fiber acceleration sensor and a micro-vibration detection system based on the vibration-sensing corrugated diaphragm. The vibration-sensing corrugated diaphragm comprises a composite film and a plurality of annular masses, the overall shape of the composite film is a disk structure with a uniform wall thickness, the lower surface of the composite film is provided with a plurality of grooves recessed upwardly, the plurality of grooves comprise a central groove and a plurality of annular grooves, the center groove and the annular grooves have the same depth, the central groove and the annular grooves are respectively arranged concentrically with the composite film, the central groove and the plurality of annulargrooves are provided with the plurality of annular masses in one-to-one correspondence, the outer wall of the annular mass is matched with the inner wall of the corresponding groove, the thickness ofthe annular mass is smaller than the depth of the corresponding groove, the upper end surface of the annular mass abuts against the top wall of the corresponding groove, and the lower end surface ofthe annular mass is higher than the lower surface of the composite film to form a corrugated shape of the diaphragm. The vibration-sensing corrugated diaphragm has the advantages of improving the sensitivity of the micro-vibration detection and realizing the miniaturization of the optical fiber acceleration sensor.
Owner:ANHUI UNIVERSITY
Rape BnPAB5 gene and application thereof
InactiveCN103045610AHigh interference efficiencyEfficient control of drug fertilityPlant peptidesFermentationPoly-A RNAPollen
The invention discloses a rape BnPAB5 gene and application of the rape BnPAB5 gene. A separated gene has the sequence as shown in SEQ ID No.1 and SE1 ID No.2. The PAB5 in combination with the poly(A) tail plays a special role in the transcript processes, such as starting of polyadenylation, transporting and translation, thus the expression processes of other genes can be controlled. The BnPAB5 shows preferential expression in rape anther and microspore. The expression of Arabidopsis AtPAB5 is interfered to result in abortion of the microspore. The transgenic arabidopsis of overexpression BnPAB5 is utilized as a male parent and pollinated to the transgenic arabidopsis of the interference AtPAB5, and the fertility of plant anther of filial generation and pollen can be recovered. As the complementary test shown, the rape BnPAB5 and the arabidopsis AtPAB5 have the same functions, such as the functions of controlling the development of the rape anther and arabidopsis anther and the fertility of the pollen; the expression level of the PAB5 can be controlled by genetic engineering technology, thus the fertility of the pollen can be controlled, and male sterility line materials and restoring line materials can be created; and the rape BnPAB5 gene can be applied to the study on male gametes development and the improvement of quality of crop.
Owner:INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
Broadband array multi-beam forming method
InactiveCN111903234BImproved beamforming accuracyImproving Beamforming AccuracyCommunication jammingFrequency coverageWide band
The invention discloses a broadband array multi-beam forming method, which relates to the simultaneous interference technology for multiple communication targets of the enemy in the field of communication. The present invention adopts a 96-element antenna array with a frequency coverage of 600-3000MHz, uses digital intermediate frequency amplitude and phase calibration technology, and can generate 8 independent and controllable beams at the same time in the 50MHz instantaneous frequency band; the interference beam is controlled by digital means at the intermediate frequency Realization, beam pointing and side lobes are flexible and controllable; the invention uses a system calibration receiver to realize online real-time calibration of system beam forming, and enhances the engineering practicability of the system. The equipment manufactured by the invention has the characteristics of flexible operation, reliable performance, high-efficiency interference and the like. It is especially suitable for jamming important communication targets in enemy theaters, and can be applied to platforms such as airborne, shipborne, and vehicle-mounted.
Owner:NO 54 INST OF CHINA ELECTRONICS SCI & TECH GRP
Method for researching helicoverpa armigera larva RNA interference efficiency
InactiveCN106222286AHigh interference efficiencyExperimental results are stableMicrobiological testing/measurementCotton bollwormOperability
The invention relates to the field of biotechnology and particularly discloses a method for researching helicoverpa armigera larva RNA interference efficiency. The method for researching the helicoverpa armigera larva RNA interference efficiency is established by improving a method for feeding helicoverpa armigera larva siRNA (small interfering RNA), the lepidopteron helicoverpa armigera larva RNA interference efficiency is improved, and experimental results are stabilized. The method provided by the invention is efficient and simple, and is high in operability, the dosage of siRNA is definite, the repeatability is high, and technical supports for researching functions of genes of lepidopteron helicoverpa armigera are provided.
Owner:HUBEI UNIV
Double optical path detection lateral shearing interferometer and interference method thereof
InactiveCN105067118AHigh interference efficiencyImprove performanceInterferometric spectrometryOptical elementsOptical pathPrism
The invention provides a double optical path detection lateral shearing interferometer, which comprises a beam-splitting cubic prism 1, a beam-splitting cubic prism 2, a right-angle reflecting prism 1 and a right-angle reflecting prism 2, wherein an incident optical axis is vertical to an incident surface of the beam-splitting cubic prism 1 and is positioned at the center of the incident surface of the beam-splitting cubic prism 1; beam-splitting surfaces of the beam-splitting cubic prism 1 and the beam-splitting cubic prism 2 are positioned on the same plane, and the beam-splitting cubic prism 2 is positioned between an emergent surface of transmitted light rays of the beam-splitting cubic prism 1 and a hypotenuse surface of the right-angle reflecting prism 1; the hypotenuse surface of the right-angle reflecting prism 1 is parallel to the incident surface of the beam-splitting cubic prism 1; the right-angle reflecting prism 2 is positioned on the upper side of the beam-splitting cubic prism 2, and a hypotenuse surface of the right-angle reflecting prism 2 is parallel to the emergent surface of reflected light rays of the beam-splitting cubic prism 1. The invention further provides a lateral shearing interference method, which has higher interference efficiency, improves performance of the interferometer, and reduces the strict requirement of the interferometer on performance of a beam splitting film.
Owner:XI'AN INST OF OPTICS & FINE MECHANICS - CHINESE ACAD OF SCI
Method for achieving radar noise interference based on multi-order inverse Fourier convolution transform
InactiveCN107656252AHigh interference efficiencyHigh densityWave based measurement systemsHigh densityRadar
The invention discloses a method for achieving the radar noise interference based on multi-order inverse Fourier convolution transform, and the method is characterized in that firstly, the method canachieve the covering of a target echo and also can resist side-lobe blanking for a high-density false target; secondly, it must be a pulse signal for resisting sidelobe cancellation; thirdly, the interference has the in-pulse modulation features the same as a target echo. The method gives consideration to the technical characteristics of noise interference and deception jamming, and the interference waveform generated by the method is better in coherence. The method enables an interference pulse signal to pass a detection processing system of a radar easily, also makes the most of the energy of an interference machine, and can achieve the purpose of effective interference.
Owner:ARMY ENG UNIV OF PLA
Interference method for inverse synthetic aperture radar based on FDA antenna
ActiveCN110471040AHigh interference efficiencyRadio wave reradiation/reflectionInterferometric synthetic aperture radarCountermeasure
The invention belongs to the technical field of radar interference and countermeasures, and relates to an interference method for an inverse synthetic aperture radar based on an FDA antenna. Accordingto the method of the invention, based on a phased array antenna, a frequency diverse array additionally adds a frequency increment that is far less than a working carrier frequency to an emission signal on adjacent array elements, an accompanying fly jammer equipped with a frequency diverse array antenna transmits an interference signal to a protected target, and the interference signal is then scattered by a target to an ISAR receiver.
Owner:UNIV OF ELECTRONICS SCI & TECH OF CHINA
Method for preventing and controlling loopworms
ActiveCN109429838AShortened spawning periodReduce in quantitySaving energy measuresHorticulture methodsBiological propertyHabit
The invention provides a method for preventing and controlling loopworms, and relates to the technical field of prevention and control of insects. The method for preventing and controlling loopworms comprises the following step: after sunset every day, illuminating the region about to prevent and control the loopworms with yellow light or green light until sunrise next day. The research show thatafter the region is illuminated with yellow light or green light at night, the spawning habit of adult loopworms can be affected remarkably, incubation of loopworm eggs can be affected remarkably, andthe emergence number of the adult loopworms can be affected remarkably. The biological characteristics of the loopworms under illumination of the yellow light or green light at night are deeply researched by a light behavior method, thus, the method for preventing and controlling the loopworms is high in selectivity and high in interference efficiency, natural enemies of the loopworms are not threatened, and ecological diversity of the insects is protected. By the method, pesticide is not required. Compared with an existing method, the method is more environmentally friendly.
Owner:XINYANG AGRI & FORESTRY UNIV
Establishment of novel RNA interference method for bemisia tabaci nymphs
InactiveCN110583581AReduce mortalityReduce experiment costOther foreign material introduction processesAnimal husbandryMortality rateBottle
The invention discloses an RNA interference method based on a bemisia tabaci nymph body wall permeation approach. The method comprises the following steps of (1) cultivation of cotton seedlings; (2) insect inoculating and spawning; (3) RNA interference based on the body wall permeation approach, wherein dsRNA and an exogenous control gene dsEGFP are prepared, the dsRNA is uniformly mixed with a nano material of a star-shaped polymer according to the volume ratio of 1:1, the mixture is carefully dropped on nymphs, cotton leaves are inserted back into a bottle filled with clean water after the liquid drops are absorbed by the insects, the insects are placed into a culture box for continuing feeding, the death number is recorded 2 days later, and nymphs samples are collected. According to themethod, by utilizing the strong permeability of the novel nano material, the dsRNA quickly permeates into bemisia tabaci bodies through the body walls, so that the death rate is obviously reduced, the interference efficiency is improved, and the timeliness and the repeatability are high. In addition, the use amount of the dsRNA in the method is very small and reaches about 0.3 microliter per individual, the interference efficiency is ensured, and meanwhile the experimental cost is greatly reduced; the method has a good application prospect.
Owner:INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
Intelligent electromagnetic information protection system
ActiveCN110311749AEnsure safetySolve the problem of selectivityCommunication jammingHigh densityBroadband power amplifier
The invention discloses an intelligent electromagnetic information protection system. The system comprises a main control module, a state detection module, a network communication module, a broadbandinterference information source generation module, a targeted comb-shaped information source generation module, a VGA interface module, a DVI interface module, an HDMI interface module, a power supplymodule, an output efficiency detection module, a power output control module, a multi-channel broadband power amplifier and an antenna. The power supply module, the network communication module, thestate detection module, the output efficiency detection module, the power output control module, the broadband interference information source generation module and the targeted comb-shaped information source generation module are all connected with the main control module. According to the system, interface electromagnetic information such as VGA, DVI, HDMI and the like can be automatically and respectively selected to be effectively protected, the interference modes of VGA, DVI and HDMI can be automatically and simultaneously started at high power, and effective electromagnetic protection can be implemented on important secret-related places, large-scale comprehensive display platforms and high-density interconnection interfaces.
Owner:成都立鑫新技术科技有限公司
SiRNA (small interfering ribose nucleic acid) for inhibiting homosapiens chromosome2open reading frame 3(C2 or f3) (GCF) gene expression, carrier of SiRNA for inhibiting GCF gene expression and application
InactiveCN102994503BInhibit expressionHigh interference efficiencyGenetic material ingredientsAntineoplastic agentsBase JGenetic engineering
The invention discloses a SiRNA (small interfering ribose nucleic acid) for inhibiting homosapiens chromosome 2 open reading frame 3(C2 or f3) (GCF) gene expression, which belongs to the field of medical genetic engineering. Deoxyribose nucleic acid (DNA) for an encoding SiRNA is selected from arbitrary group of (a), (b) and (c): (a) a base sequence of a positive-sense strand is shown as SEQ ID No.1, and the base sequence of an antisense strand is shown as SEQ ID No.2; (b) the base sequence of the positive-sense strand is shown as SEQ ID No.3, and the base sequence of the antisense strand is shown as SEQ ID No.4; and (c) the base sequence of the positive-sense strand is shown as SEQ ID No.5, and the base sequence of the antisense strand is shown as SEQ ID No.6. The invention further discloses a recombinant plasmid which comprises the DNA of the encoding SiRNA and a GCF-shRNA-HeLaCGMCC (China general microbiological culture collection center) No.5821, and a cell line has high RNA interfering efficiency, strong specificity and no toxic and side effects and can effectively inhibit GCF gene expression. The SiRNA for inhibiting GCF gene expression, the recombinant plasmid and the GCF-shRNA-HeLa monoclonal cell can also be used for preparing medicines for treating cervical cancer.
Owner:NAT INST FOR RADIOLOGICAL PROTECTION & NUCLEAR SAFETY CHINESE CENT FOR DISEASE CONTROL & PREVENTION +2
Rule weight distribution siRNA design method based on grid search
PendingCN112951322AQuantitative prediction efficiencyAvoid base biasCharacter and pattern recognitionProteomicsAlgorithmInformatics
The invention discloses a rule weight distribution siRNA design method based on grid search, and relates to the technical field of bioinformatics. The method comprises the following steps: S1, training siRNA data to obtain siRNA design rule weights; and S2, based on the obtained siRNA design rule weights, screening out siRNA with high silence efficiency from the candidate siRNA set. According to the method, each rule is differentially treated according to different weights, and based on the multi-rule design of different weights, all rules can be prevented from being treated according to the same importance degree, important rules in the rules can be highlighted, effective siRNA and ineffective siRNA can be distinguished, and the efficiency of candidate siRNA sequences can be quantitatively predicted.
Owner:深圳市新合生物医疗科技有限公司
Small RNA for human and rat-homologous NMDA receptor NR2B and application thereof
InactiveCN101942444AAchieving the goal of treating chronic painConvenient route of administrationNervous disorderGenetic material ingredientsNR1 NMDA receptorSide effect
The invention belongs to the field of biological genes. Chronic pain is one of forms of expression for neuronal plasticity change, and has the physiological characteristics of increased reactivity of pain sense, treatment difficulty, unclear mechanism, and more side effects by the use of opiate receptor antagonist in treatment. Researches prove that the NMDA regulation is related to the chronic pain. Therefore, the invention provides a small RNA for a human and rat-homologous NMDA receptor NR2B, which has a sequence shown as SEQ ID NO:1. The invention also provides application of the small RNA to medicaments for treating the chronic pain. The small RNA can be directly injected, can inhibit the expression of the NMDA receptor NR2B for human and a rat in vitro and in vivo to achieve the effect of treating the chronic pain, and has the advantages of convenient administration route, no addiction, tolerance and other reactions, and long-term use.
Owner:俞卫锋 +1
Intelligent Electromagnetic Information Protection System
ActiveCN110311749BEnsure safetySolve the problem of selectivityCommunication jammingInterference (communication)Broadband power amplifier
The invention discloses an intelligent electromagnetic information protection system, which includes a main control module, a state detection module, a network communication module, a broadband interference source generation module, a targeted comb source generation module, a VGA interface module, a DVI interface module, an HDMI Interface module, power supply module, output performance detection module, power output control module, multi-channel broadband power amplifier and antenna, the power supply module, network communication module, state detection module, output performance detection module, power output control module, broadband interference signal Both the source generation module and the target comb source generation module are connected with the main control module. The present invention can not only automatically select and effectively protect the electromagnetic information of interfaces such as VGA, DVI, and HDMI, but also automatically and simultaneously turn on the interference mode of VGA, DVI, and HDMI at high power. The interface implements effective electromagnetic protection.
Owner:成都立鑫新技术科技有限公司
A vibration-sensing corrugated diaphragm, an optical fiber acceleration sensor and a micro-vibration detection system
ActiveCN108663538BImprove micro-vibration sensing sensitivityFacilitated releaseAcceleration measurement using interia forcesSubsonic/sonic/ultrasonic wave measurementComposite filmThin membrane
Owner:ANHUI UNIVERSITY
An Inverse Synthetic Aperture Radar Jamming Method Based on FDA Antenna
ActiveCN110471040BHigh interference efficiencyRadio wave reradiation/reflectionSynthetic aperture radarSignal on
The invention belongs to the technical field of radar interference countermeasures, and relates to an inverse synthetic aperture radar interference method based on an FDA antenna. In the method of the invention, on the basis of the phased array antenna, the frequency-controlled array adds a frequency increment far smaller than the working carrier frequency to the transmitted signal on the adjacent array elements, and the accompanying jammer equipped with the frequency-controlled array antenna The jamming signal is transmitted to the protected target, and then scattered by the target to the ISAR receiver.
Owner:UNIV OF ELECTRONICS SCI & TECH OF CHINA
Construction and application of GADD45betaRNA interference report vector based on EGFP
InactiveCN104894164AHigh interference efficiencyVector-based foreign material introductionForeign genetic material cellsOpen reading frameBladder cancer cell
The invention relates to construction of a GADD45betaRNA interference report vector based on EGFP and to a method of fast screening siRNA. The method can be used for studying gene function at a cellular level and an in-vivo level. By inserting a GADD45beta open reading frame into a pEGFP-N1 vector, the GADD45betaRNA interference report vector, of p-GADD45beta-EGFP-N1, based on EGFP is constructed, and fusion expression of GADD45beta and EGFP is carried out in the vector. The vector together with the synthetic GADD45betasiRNA cotransfects the bladder cancer cell line T24, and it is found out that interfering effects of GADD45betasiRNA360 and siRNA638 are best. Compared with a conventional qPCR method, the method in the invention is time saving and labor saving. The method is suitable for fast screening siRNA in bladder cancer cells and mammalian cells and is used for studying gene function.
Owner:SHANXI UNIV
A system and method for generating counter-electromagnetic waveforms for black-flying drones
ActiveCN111800216BExtended service lifeHigh interference efficiencyCommunication jammingNeural architecturesTelecommunications linkInterference (communication)
The present invention provides a generation system and method for anti-electromagnetic waveforms of black flying drones. The system includes a monitoring module, a link feature identification module, and an interference handling module, wherein the monitoring module is used to receive wireless The electromagnetic wave signal between the man-machine and the remote control is input to the link feature identification module; the link feature identification module is used to blindly identify the modulation and frame format of the data link used by the drone from the intercepted electromagnetic wave signal , coding, and signal bandwidth; the interference handling module is used to regenerate the interference waveform according to the identification result, and send it to the air to counter the UAV. Beneficial effects of the present invention: the four basic parameters of the communication link layer used by the UAV can be quickly identified, and based on this, an interference waveform signal with a high signal-to-noise ratio and a high similarity to the real waveform can be reproduced, which can be used to distinguish the real from the real, and improve Interference efficiency, while reducing interference power, prolongs the service life of the device.
Owner:SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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