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Method for testing biotoxicity of oil-field wastewater by employing vibrio qinghaiensis Q67

A technology for oil extraction wastewater and Vibrio qinghai, which is applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as no wastewater toxicity discharge indicators, etc., and achieves easy popularization and application, stable experimental results, and moderate toxicity. Effect

Active Publication Date: 2017-05-31
GUILIN UNIVERSITY OF TECHNOLOGY
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Problems solved by technology

The existing "Comprehensive Wastewater Discharge Standard" (GB 8978-1996) stipulates the physical and chemical index limits of wastewater discharge, but does not involve the discharge indicators of wastewater toxicity

Method used

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  • Method for testing biotoxicity of oil-field wastewater by employing vibrio qinghaiensis Q67

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Embodiment

[0019] The wastewater samples to be tested in this embodiment are taken from the various treatment process sections of oil production wastewater in a certain petroleum Weizhou terminal treatment plant. The five sampling points are: raw water, ABR pool water, ABR pool effluent, SBR pool water and effluent. The samples were tested as follows:

[0020] (1) Take the oil production wastewater of each process section as the test wastewater and perform pretreatment:

[0021] After the oil production wastewater is transported to the terminal treatment plant through pipelines, it is biochemically treated by the combined process of anaerobic baffle reactor and activated sludge method, and the wastewater treated by biochemical method is transported to the clear water tank and then discharged outside the plant. In order to avoid the influence of turbid wastewater on the experimental results, the sample to be tested was filtered with a 0.45 μm filter membrane and stored in a refrigerator a...

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Abstract

The invention discloses a method for testing biotoxicity of oil-field wastewater by employing vibrio qinghaiensis Q67. The oil-field wastewater is taken as test wastewater, the vibrio qinghaiensis Q67 is taken as a bioindicator and a 96-pore microwell plate is taken as a carrier; by adopting a 96-pore microwell plate analyzing method, the luminous intensity is detected by adopting a microwell plate spectraphotometer; and the luminescent inhibition rate is calculated by using the luminous intensity of an experiment group and a control group and Zn(NO3)2 is set as a toxic reference substance, thereby judging the biotoxicity of the oil-field wastewater. According to the method, the toxicity of the oil-field wastewater is detected and analyzed by using a microplate method; and the comprehensive toxicity of the oil-field wastewater is judged through calculating the luminescent inhibition rate of luminescent bacteria; the problem that conventional physicochemical indexes cannot represent the biotoxicity of the oil-field wastewater is solved; the method is fast, sensitive and convenient to popularize and apply; the selected toxic reference substance Zn(NO3)2 is steady in experiment result, low in price and medium in toxicity.

Description

technical field [0001] The invention belongs to the field of wastewater biotoxicity detection, in particular to a method for testing the biotoxicity of oil production wastewater by using Vibrio Q67 of Qinghai. Background technique [0002] Oil production wastewater is the wastewater that needs to be discharged outside during the oil production process of the oil field, except for production water such as reinjection, process remixing or other purposes. It contains high molecular refractory organic pollutants such as petroleum and surfactants. Oil extraction wastewater has the characteristics of high salinity and high water temperature, and is corrosive to objects in contact. In addition, the wastewater also contains a certain amount of fungicides. The existing "Comprehensive Wastewater Discharge Standard" (GB 8978-1996) stipulates the physical and chemical index limits of wastewater discharge, but does not involve the discharge indicators of wastewater toxicity. At present...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/64C12Q1/02
CPCC12Q1/02C12Q1/64
Inventor 李艳红肖楠张立浩覃礼堂张鑫宋晓红崔耀宗
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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