Method for screening and identifying potassium-rich tobacco

Abstract

The invention provides a method for screening and identifying potassium-rich tobacco. The method comprises steps as follows: 1) tobacco seeds are sown on a low-potassium culture medium for seedling culture, tobacco seedlings are graded according to the reaction degree of tobacco seedlings to low potassium, tobacco plants which have high yellowing degree and are active are screened and transplanted to a full nutrient solution for growth; 2) when the tobacco seedlings grow to have 6-8 leaves, the tobacco seedlings are transplanted to a field, tobacco leaves in the same leaf positions of the selected plants are subjected to fixation, drying, grinding and sieving in the manure period, the content of potassium is measured, and the plant with the highest potassium content is labeled; 3) seeds of the plant with the highest potassium content are harvested, planting and screening in step 1) and step 2) are performed repeatedly until genotype homogeneity is realized, and the variety of potassium-rich tobaccos is bred. Based on the principle that potassium-rich variants are not resistant to low potassium, potassium-rich tobacco variant materials are screened by use of the low-potassium culture medium, field verification is combined in the later period, and the result is accurate and stable.

Description

technical field [0001] The invention relates to the field of tobacco heredity and variety breeding, in particular to a method for screening and identifying potassium-rich tobacco. Background technique [0002] Potassium is one of the most important quality elements of tobacco, which is closely related to the maturity, combustibility, taste and safety of tobacco leaves. Compared with the potassium content of foreign tobacco leaves (>3%), the average potassium content of tobacco leaves rarely exceeds 1.5% in most areas of China, especially the northern regions. The low potassium content of tobacco leaves is one of the key indicators restricting the improvement of tobacco leaf quality in China. To produce high-quality tobacco leaves, it is necessary to apply potassium fertilizers 2 to 3 times the amount of potassium required to form the optimum yield in order to significantly increase the potassium content of tobacco leaves. Breeding flue-cured tobacco potassium high-effici...

AI Technical Summary

Problems solved by technology

However, the conventional hybridization screening method must carry out a large number of tests after the tobacco leaves are harvested to know the result. Therefore, the blindness is large and the workload is large.

[0005] 2. Transgenic method: Using genetic engineering technology to transfer some potassium-efficient genes into other plants and induce their expression, you can quickly and efficiently obtain potassium-efficient plants. However, tobacco transgenics are currently restricted in the world for production, so this technology cannot use

If the genes related to potassium absorption and transport are simply determined, the variation type of potassium-rich tobacco may not be obtained, that is, some test results are not consistent with the results of field experiments. This is the main reason why potassium-rich tobacco cannot be bred at present.

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