121 results about "Two dimensional electrophoresis" patented technology

The present invention provides methods and compositions for screening, diagnosis and prognosis of Schizophrenia, for monitoring the effectiveness of Schizophrenia treatment, identifying patients most likely to respond to a particular therapeutic treatment and for drug development. Schizophrenia-Associated Features (SFs), detectable by two-dimensional electrophoresis of cerebrospinal fluid, serum or plasma are described. The invention further provides Schizophrenia-Associated Protein Isoforms (SPIs) detectable in cerebrospinal fluid, serum or plasma, preparations comprising isolated SPIs, antibodies immunospecific for SPIs, and kits comprising the aforesaid.

The present invention provides an integrated, fully automated, high-throughput system for two-dimensional electrophoresis comprised of gel-making machines, gel processing machines, gel compositions and geometries, gel handling systems, sample preparation systems, software and methods. The system is capable of continuous operation at high-throughput to allow construction of large quantitative data sets.

The present invention provides an integrated, fully automated, high-throughput system for two-dimensional electrophoresis comprised of gel-making machines, gel processing machines, gel compositions and geometries, gel handling systems, sample preparation systems, software and methods. The system is capable of continuous operation at high-throughput to allow construction of large quantitative data sets.

The present invention is to provide a sample separation instrument used in a sample separation apparatus which includes: holding means for holding a first medium supporter which supports a first medium; and driving means for moving fixing means or the holding means in a direction parallel or perpendicular to a plane whose sides extend in the first direction and in the second directions. The sample separation instrument includes an insulator for storing a second medium which allows a sample separated in the first medium in the first direction to be further separated in the second direction different from the first direction, wherein: the insulator includes a first opening and a second opening each of which defines the second direction in which the second medium is electrified, and the second opening has a shape which allows the first medium containing the separated sample to be attached to the second medium along the first direction. The present invention realizes automation of the two-dimensional electrophoresis to enhance the convenience of the two-dimensional electrophoresis, to less depend on the operator's skill, and to enhance the reproducibility of the electrophoresis result.

An ensemble of components and methods are disclosed for utilizing two-dimensional electrophoresis in polyacrylamide or related polymer gels using common, existing and familiar electrophoresis formats and equipment. The disclosed invention makes two-dimensional electrophoresis convenient and easy to use for individuals already using vertical “mini-gel” type systems. The invention discloses the combination of pre-cast disposable gels for both first and second separation dimensions using novel support devices and cassettes that simplify the difficult multiple sample handling and processing steps inherent in ordinary two-dimensional electrophoresis methods. Devices and methods are disclosed in said invention that provide exceptional gel to gel reproducibility.

The present invention provides methods and compositions for screening, diagnosis and prognosis of Alzheimer's disease, for monitoring the effectiveness of Alzheimer's disease treatment and for drug development. Alzheimer's disease-Associated Features (ADFs) detectable by two-dimensional electrophoresis of brain tissue are described. The invention further provides Alzheimer's disease-Associated Protein Isoforms (ADPIs) detectable in brain tissue, preparations comprising isolated ADPIs, antibodies specific for ADPIs and kits comprising the aforesaid.

The present invention provides methods and compositions for screening, diagnosis and prognosis of Schizophrenia, for monitoring the effectiveness of Schizophrenia treatment, identifying patients most likely to respond to a particular therapeutic treatment and for drug development. Schizophrenia-Associated Features (SFs), detectable by two-dimensional electrophoresis of cerebrospinal fluid, serum or plasma are described. The invention further provides Schizophrenia-Associated Protein Isoforms (SPIs) detectable in cerebrospinal fluid, serum or plasma, preparations comprising isolated SPIs, antibodies immunospecific for SPIs, and kits comprising the aforesaid.

A device for two-dimensional electrophoresis includes a cassette comprising two opposing plates. The two opposing plates form a first elongated portion for receiving a first elongated electrophoretic separation medium and a second portion extending away from the first portion. A second electrophoretic separation medium is on the second portion and between the two opposing plates. A dialysis membrane extends across the second electrophoretic separation medium.

The invention discloses a method for acquiring a two-dimensional strawberry electrophoresis differential protein map. The method comprises the following steps: (1) extracting proteins; (2) performing first-dimensional IPG isoelectric focusing electrophoresis; (3) balancing an adhesive tape; (4) transferring the adhesive tape and performing SDS-polyacrylamide gel electrophoresis; and (5) performing gel scanning and image analysis. An inventor considers the characteristic that strawberry leaves are rich in polysaccharide polyphenol substances in the invention process, the strawberry leaf protein is subjected to repeated precipitation extraction by virtue of acetone, the lysate formula is improved, the centrifugal rate, the cleaning frequency of crude protein precipitations and concentration of urea in lysate are improved, and a set of differential two-dimensional electrophoresis proteomics method suitable for strawberry leaves is constructed, so that the high-quality strawberry protein differential map is obtained. The method can be used for obtaining the two-dimensional strawberry leaf protein electrophoresis gel map with clear protein points, a large number of proteins, uniform protein distribution and a clear background.

The invention discloses an extraction method for mangrove plant kandelia candel leaf total protein suitable for two-dimensional electrophoresis. A traditional trichloroacetic acid / acetone precipitation method for protein extraction and a traditional phenol extraction method for protein extraction are combined, a cosolvent SDS solution is introduced, the extraction process is optimized, and the Phe-B method suitable for extraction of the mangrove plant kandelia candel leaf total protein is established. The extraction efficiency and quality of the protein are effectively improved, and the technical scheme can be specifically suitable for extraction of the kandelia candel leaf protein in two-dimensional electrophoresis. The method has the advantages of being easy to operate, high in protein extraction efficiency and fewer in interfering substance, and is suitable for materials with difficult protein extraction, low protein extraction efficiency and more interfering substances. The extracted kandelia candel leaf protein completely meets the requirements of the first direction and the second direction of two-dimensional electrophoresis, a high-quality two-dimensional electrophoresis gel map with high resolution, more clear protein points, uniform distribution and the clear background can be obtained, and experiment repeatability and stability are good.

The invention discloses a paper base micro-fluidic chip for selecting soil active bacterium and components and application of the paper base micro-fluidic chip. A plurality of micro-fluidic units, and units, such as carbon paste three-electrode arrays for bacterial respiration electrochemical measurement are printed on paper to form two types of array dual paper base micro-chamber co-culture and activity screening function units, on chromatographic paper, a carbon paste electrode, a PDMS (Polydimethylsiloxane) passage, a PDMS micro-culture room and the like are sequentially overprinted through silk-screen printing, and furthermore, a model bacterial array unit is printed in an aseptic condition; a dual micro-chamber co-culture array unit of a sample bacterium-model bacterium and a secondary metabolite of a sample bacterium are used to inhibit adjacent model bacterium respiratory chains, and a carbon paste three-electrode printed together with a dual micro-chamber is used to find the position of some active sample bacterial colony; the secondary metabolite of the active sample bacterium is separated through paper base two-dimensional electrophoresis, and an array carbon paste three-electrode is used to find the positions of active components obtained through two-dimensional electrophoresis. The paper base micro-fluidic chip for selecting soil active bacterium is suitable for selecting the active bacterium and active complex components in multiple fields.

The invention discloses a mycoplasma bovis immunity-related protein P22, a nucleotide sequence for coding the same and an application thereof. A mycoplasma bovis Hubei strain is taken as a sample, and an efficient two-dimensional electrophoresis system of a mycoplasma bovis whole protein is established, so that a two-dimensional electrophoretogram with high resolution and high repeatability is obtained; an immunity-related protein is screened in combination with Western blot, and is named P22 protein; and as proved by a prokaryotic expression result of the protein, a Western blot rest result of a recombinant protein and a mycoplasma bovis positive serum is negative, and a Dot blot test result is positive. The protein is proved to be possibly a conformation-dependent immunity-related protein of mycoplasma bovis, and the mycoplasma bovis immunity-related protein P22 plays a guidance role in diagnosing, preventing and treating a mycoplasma bovis disease.

The invention relates to the protein two-dimensional electrophoresis technology of a Mongolian ammopiptanthus, which adopts a two-dimensional electrophoresis technology to prepare the protein of a plant living in extreme environment. The experiment carried out to the Xinjiang ammopiptanthus living in the extreme environment by the technical proposal achieves good effect. At present, repeated experiments certify that: the two-dimensional electrophoresis technology has complete prepared laboratory samples, good repeatability, clear atlas and reliable results, can provide technical examples for the research of plant proteomics threatened by adverse circumstances and is a two-dimensional electrophoresis technology applicable to the proteomic analysis of the Xinjiang ammopiptanthus.

An adaptor is designed as an accessory to an ultraviolet transilluminator for the excitation of fluorescent molecules or labels in a planar array of biochemical samples such as a two-dimensional electrophoresis gel to enable the emissions resulting from the excitation to be detected and quantified. The adaptor is constructed to overlay the transilluminator and contains both a fluorescent dye that upon excitation by ultraviolet light emits light in the visible spectrum, and a conditioning substance that selects a portion of the wavelength band of the visible light produced by the fluorescent dye. The adaptor converts the ultraviolet light from the transilluminator to visible light while limiting the emissions reaching the detector to those that emanate from the sample. By the use of this adaptor, the transilluminator is adapted for use with samples labeled with dyes that are excitable by visible light and avoids exposure of the samples and the user to ultraviolet light.

The invention provides a method for identification of barley varieties. The method comprises: peeling and crushing pure barley, then adding a buffer solution to perform extraction, then carrying out purification through a series of steps so as to obtain water-soluble protein of barley, subjecting different varieties of barley protein to electrophoresis by a two-dimensional electrophoresis technology so as to obtain clear and visualized protein maps, and carrying out contrastive analysis to search differential protein of different varieties, thus effectively identifying barley varieties.

The invention discloses a broad bean leaf proteomics analysis sample preparation method which comprises the following steps: taking fresh broad bean seedling leaves; cooling the fresh broad bean seedling leaves through liquid nitrogen and then grinding the cooled fresh broad bean seedling leaves into powder; adding an extracting buffer solution, after precipitation, taking supernatant, carrying out centrifugation, and removing the supernatant after centrifugation so as to obtain precipitates; adding an acetone solution, carrying out standing and centrifugation, and removing the supernatant so as to obtain precipitates; drying the precipitates to obtain powder; adding lysate into the powder; centrifuging, taking the supernatant, centrifuging again and taking the supernatant as a to-be-analyzed solution; measuring the concentration of protein in the to-be-analyzed solution; calculating the volume of the protein and sub-packaging for storage; adding a protein sample hydration solution for hydration and centrifuging so as to obtain a broad bean leaf proteomics analysis sample. With the adoption of the broad bean leaf proteomics analysis sample preparation method disclosed by the invention, the blank of a two-dimensional electrophoresis technology of broad bean leaves is filled, and the technical support is provided for deeply developing broad bean proteomics. With the adoption of the technical scheme, the steps are simple, the operation is easy, the repeatability is good, and technicians can learn and master easily. Used experiment reagents are two-dimensional electrophoresis conventional reagents, expensive and rare reagents are avoided and the experimental operating cost is low.

The invention provides an extraction method of zizania latifolia protein in the interaction of zizania latifolia and zizania smut, which simulates the interaction between zizania latifolia and zizania smut, to extract zizania latifolia gene groups and proteins which are not mixed. The invention can prepare the zizania total protein product without the protein of zizania smut, whose protein content is high, impurity content is low and nucleic acid content is low, thereby satisfying the demand of two-dimensional electrophoresis to protein purity.

The invention provides a buildup method of a protein difference expression atlas when the committed differentiation of stem cells is induced by drugs, which detailedly establishes the protein difference expression atlas formed by that the stem cell is induced by the ICA and directionally differentiated to be a cardiac muscle cell; a model that the drug induces the stem cell to be directionally differentiated to be the cardiac muscle cell is established so as to prepare a two-dimensional electrophoresis protein sample; electrophoresis is gelled bidirectionally, images are collected and analyzed so as to confirm the difference protein. A comparison protein atlas is used for screening and identifying the difference expression protein when the ES cell is directionally differentiated to be the cardiac muscle cell by the inducing of the ICA; the difference expression protein is used as a drug effect target and applied to the preparation of a novel inducer which has high-efficiency and low-toxicity and is used for prompting the stem cell to be differentiated.

The present invention provides methods and compositions for screening, diagnosis and prognosis of BAD, for monitoring the effectiveness of BAD treatment, and for drug development. BAD-Associated Features (DFs), detectable by two-dimensional electrophoresis of cerebrospinal fluid, serum or plasma are described. The invention further provides BAD-Associated Protein Isoforms (DPIs) detectable in cerebrospinal fluid, serum or plasma, preparations comprising isolated DPIs, antibodies immunospecific for DPIs, and kits comprising the aforesaid.

The present invention discloses a diagnosis antigen for Toxoplasma gondii infection and a preparation method and applications thereof, belonging to the technical field of biological veterinary drugs. An antigen GRA8 suitable for diagnosis for Toxoplasma gondii infection is screened out by using methods combining Western blot, two-dimensional electrophoresis, and MALDI-TOF-TOF tandem mass spectrometry. The antigen can be used for a variety of molecular biology diagnosis and serological diagnosis for the Toxoplasma gondii infection.

The invention discloses a method for screening and identifying an ECH1 autoantibody and an application thereof. The method comprises the following steps: adopting a lung cancer cell line H1299 holoprotein extractive for performing two-dimensional electrophoresis; adopting a western blot method for processing the serum of 5 patients with lung cancer and serum of 5 normal people, screening the significant tumor autoantibody, and after comparative analysis, only further analyzing the protein spots appearing in the serum of the patients; cutting and digesting the interesting protein spots on SDS-PAGE gelatin, and then adopting a LG-MS / MS mass spectrometry method for analyzing the protein spots, detecting and identifying as ECH1; and further verifying if the ECH1 autoantibody can be used as a lung cancer diagnosis marker. Through research, the invention finds that the detection for the serum ECH1 autoantibody according to an ELISA method can be used for more accurately identifying the patients with lung cancer from the normal people, identifying the patients with chronic obstructive pulmonary diseases and early diagnosing the lung cancer.

The invention provides a method for detecting barley seed purity. The method comprises the steps of: peeling and crushing pure barley and then adding a buffer solution to extract; purifying by a series of steps to obtain soluble protein of barley; and carrying out two-dimensional electrophoresis on different purities of barley which is interbred by manpower, and analyzing a two-dimensional electrophoresis chart to find out the interbred different protein points. The manually interbred three-dimensional (3D) effect picture is analyzed by application of PDQuest software; and the purity of the barley seed can be accurately detected by a standard curve of height and purity curves of different protein points.

Methods are provided for separating linear nucleic acid fragments based on their conformation. The methods are based on novel two-dimensional gel electrophoresis techniques. In one aspect the method comprises electrophoresing a sample of nucleic acids in a first dimension said sample through a gel matrix under a first set of pre-determined electrophoresis conditions; electrophoresing said gel matrix in a second dimension under a second set of electrophoresis conditions, such that linear nucleic acid fragments of equal length but having different conformation are separated; said first and second electrophoresis conditions are different, such that in one of said dimensions electrophoresis allows separation of linear nucleic acid fragments based on conformation and length, and in the other of said dimensions electrophoresis allows separation of the sample fragments based substantially on length. Said difference is preferably established with a chemical agent capable of reducing conformational differences between linear nucleic acids fragments.

After the sequencing of the human genome, great interest has developed in trying to discern the complementary proteome of humans and other species. The present disclosure provides devices, systems, and methods for proteomic fractionation that may increase the number of protein spots visualized by 2DE analysis, and may allow enrichment of proteins normally not detectable by standard 2DE analysis. According to some embodiments of the disclosure, devices, systems, and methods of the disclosure relate to fractionating a proteome on the basis of surface charge, hydrophobicity, isoelectric point and / or size.

The present invention describes rhodamine compounds particularly well-suited for pre-labeling a protein that is then subjected to two-dimensional electrophoresis. Isomeric purification and synthesis of a dye having net neutral charge or no charge precludes interference with isoelectric electrophoretic separation.

The invention discloses a method for identifying whether wheat to be tested is Pina-D1b deficient wheat and applications thereof. The method according to the invention comprises the following steps: 1) using a specific primer pair for PCR amplification by taking genome DNA of the wheat to be tested as a template, wherein the specific primer pair is the primer pair composed of nucleotide sequences, which are shown in sequence 1 and sequence 2 of sequence table; and 2) detecting PCR-amplified product, wherein the wheat to be tested with 776bp strips in the amplified product is candidate Pina-D1b deficient wheat, and the Pina-D1b deficient wheat is the wheat having nucleotides, which are shown in sequence 3 of the deficient sequence table of the genome DNA. The specific primer pair according to the invention can take the place of traditional SDS-PAGE and two-dimensional electrophoresis technology and the detection for Pina-D1b type is directly carried out at molecular level, so accuracy and reliability are greatly enhanced, screening efficiency and accuracy for grain hardness genotype is remarkably quickened; in addition, the method plays an important role in improving gene engineering of wheat grain hardness, simultaneously aids in further research on genetics and molecular biology of wheat grain hardness and is also significant to the cultivation of high-quality wheat.