Paper base micro-fluidic chip for selecting soil active bacterium and components and application of paper base micro-fluidic chip

Abstract

The invention discloses a paper base micro-fluidic chip for selecting soil active bacterium and components and application of the paper base micro-fluidic chip. A plurality of micro-fluidic units, and units, such as carbon paste three-electrode arrays for bacterial respiration electrochemical measurement are printed on paper to form two types of array dual paper base micro-chamber co-culture and activity screening function units, on chromatographic paper, a carbon paste electrode, a PDMS (Polydimethylsiloxane) passage, a PDMS micro-culture room and the like are sequentially overprinted through silk-screen printing, and furthermore, a model bacterial array unit is printed in an aseptic condition; a dual micro-chamber co-culture array unit of a sample bacterium-model bacterium and a secondary metabolite of a sample bacterium are used to inhibit adjacent model bacterium respiratory chains, and a carbon paste three-electrode printed together with a dual micro-chamber is used to find the position of some active sample bacterial colony; the secondary metabolite of the active sample bacterium is separated through paper base two-dimensional electrophoresis, and an array carbon paste three-electrode is used to find the positions of active components obtained through two-dimensional electrophoresis. The paper base micro-fluidic chip for selecting soil active bacterium is suitable for selecting the active bacterium and active complex components in multiple fields.

Description

technical field [0001] The present invention relates to microbial screening of active bacteria and further screening of active ingredients, in particular to the preparation and application of a paper-based microfluidic chip for screening soil active bacteria and ingredients, mainly a silk screen overprint on paper including sample bacteria-model bacteria Paper-based 2-dimensional electrophoresis microfluidic chip for co-cultivation of dual microchambers, dual microchambers of model bacteria, 2-dimensional electrophoresis, and bacterial respiration electrochemical measurement array. Background technique [0002] In 1928, Alexander Fleming discovered penicillin and the inhibition zone (A. Fleming, 1929, 12, 226-236). Twelve years later, Florey and Chain separated and obtained the first clinically applied antibiotic --- penicillin. Lewis (K.Lewis, 2012, 485, 439-440) pointed out that in the golden age of antibiotic discovery (40s to 60s), most antibiotics were screened from so...

AI Technical Summary

Problems solved by technology

[0007] In view of the difficulty, low probability, and time-consuming and labor-intensive defects in obtaining antibiotic active bacteria and active antibiotic components in polluted soil, the purpose of the present invention is to provide a paper-based microfluidic chip for screening soil active bacteria and components. Preparation and application, through Multiple overprinting of bacteria-gelatin array culture, PDMS 2-dimensional electrophoresis, carbon slurry electrophoresis electrodes and electrochemical three-electrode array unit on chromatographic paper to obtain a paper-based microfluidic chip, and then use the array paired sample bacteria-model bacteria paper base Microchambers co-cultivate and screen antibiotic active bacteria from soil samples, and then capture them in situ using paper-based electrophoresis with 2-dimensional vertical channels and array microchambers of model bacteria that are evenly distributed on paper-based 2-dimensional planes or discover 2-dimensionally separated active ingredients

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