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Blood type detection method based on membrane structure

A detection method and membrane structure technology, applied in the direction of measuring devices, biological tests, material inspection products, etc., can solve the problems of limited application and long time consumption, and achieve the effects of objective results, short detection time, and good clinical promotion value

Inactive Publication Date: 2015-06-17
TIANJIN DEXIANG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The column agglutination method requires the use of a centrifuge, which takes a long time and limits its application in emergency situations

Method used

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  • Blood type detection method based on membrane structure
  • Blood type detection method based on membrane structure
  • Blood type detection method based on membrane structure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A blood type detection method based on membrane structure. This method uses a filter membrane that allows a single red blood cell to pass through as the core material. The blood group antibody is bound to the filter membrane. The filter membrane is surrounded by water-absorbing materials. Drop it on the surface of the filter membrane and rinse it with a washing solution. The red blood cells that have not been combined with antigen and antibody will leak out with the washing solution, and the red blood cells that have been bound by antigen and antibody will stay on the surface of the membrane to form visible red clumps. The operator can judge the result based on whether the red blood cells form visible red clumps on the filter membrane and determine the blood type of the red blood cells. Among them, the method uses a filter membrane that allows a single red blood cell to pass as the core material, and the filter membrane material can be glass fiber membrane, nitrocellulose...

Embodiment 2

[0030] The method also includes a detection kit. The detection kit is equipped with a filter membrane that allows a single red blood cell to pass through, and binds the blood group antibody to the filter membrane. A water-absorbing pad is placed under the filter membrane. The length and width of the pad are larger than the filter membrane, that is, the filter membrane is located in the center area of ​​the absorbent pad, such as figure 2 As shown, the others are the same as in Example 1.

Embodiment 3

[0032] ABO and Rh(D) blood group detection kit, select glass fiber membrane as filter membrane;

[0033] 1 Fix blood group antibodies (anti-A, anti-B, anti-D) on the glass fiber membrane

[0034] Blood group antibody fixation: Take high titer blood group monoclonal antibodies (anti-A, anti-B, anti-D) to add antibody protective agent. The antibody protective agent can be a 5 mM phosphate buffer (pH 7.4) containing 5% bovine serum albumin, 5% sucrose, 5% PVP, and 5% calf serum. The antibody protection solution can help the antibody to be fixed on the glass fiber membrane and enhance the validity of the antibody. Use the antibody protection solution as the diluent to dilute the antibody to a final titer of 128. Add 150 μL of the diluted antibody dropwise to the center of the 1.5cm×1.5cm glass fiber membrane, spread the antibody on the glass fiber membrane in a round shape, and then dry it at 37°C and seal it for storage.

[0035] 2Assembly of rapid test reagent strip:

[0036] Paste a...

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Abstract

The present invention discloses a blood type detection method based on a membrane structure. According to the method, a filtration membrane allowing a single red blood cell to pass through is selected as a core material, blood type antibodies are bound to the filtration membrane, a water absorption material is surrounding the periphery of the filtration membrane, red blood cells with a certain concentration are added on the filtration membrane surface in a dropwise manner during detecting, rinsing is performed with a rinsing liquid, the red blood cells with no antigen-antibody binding exude along with the rinsing liquid, the red blood cells with the antigen-antibody binding are remained on the membrane surface so as to form the visible red clumps, and an operator can judge the result according to whether the red blood cells form the visible red clumps on the filtration membrane so as to determine the blood type of red blood cells. The method of the present invention has beneficial effects of short detection time and objective result.

Description

Technical field [0001] The invention relates to a blood type determination and detection technology, in particular to a blood type detection method based on a membrane structure. Background technique [0002] Blood group testing has been widely used in medical institutions at all levels, and the ABO blood group system and anti-Rh(D) testing have been used as routine tests. IgM types of anti-A, anti-B and anti-D reagents are usually used to react with red blood cells respectively, and type the red blood cells according to whether they form an aggregation reaction. Type A red blood cells have A antigen on their surface, which will react with anti-A antibodies. Type B red blood cells have B antigen on their surface, which will react with anti-B antibodies. According to the results of these two reactions, the ABO blood group system of red blood cells can be classified. Use anti-D reagent to determine Rh(D) positive and negative. The detection of other blood group systems is also ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/80G01N33/543
CPCG01N33/80G01N33/54306
Inventor 黄志刚
Owner TIANJIN DEXIANG BIOTECHNOLOGY CO LTD
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