31results about How to "Good clinical promotion value" patented technology

The invention discloses a kit and a method for staining Mycobacterium tuberculosis and its expressed protein. The present invention provides a method for staining Mycobacterium tuberculosis and its expressed protein, comprising the following steps: 1) sequentially incubating a sample containing Mycobacterium tuberculosis with a primary antibody and a secondary antibody to obtain an antibody binding product; 2) ) The antibody-binding product is sequentially stained with DAB staining solution, phenolic fuchsin staining solution and hematoxylin staining solution to achieve staining of Mycobacterium tuberculosis and its expressed protein. The experiments of the present invention prove that the present invention has developed a new kit for pathological diagnosis of tuberculosis, which can simultaneously detect Mycobacterium tuberculosis cells and their expressed proteins in a tissue specimen slice, Thereby improving the sensitivity of pathological diagnosis of tuberculosis. The kit is easy to use, suitable for routine detection in pathology department, and has good clinical promotion value.

The invention discloses a construction method and application of a bispecific chimeric antigen receptor recombinant gene for treating HIV infection. The bispecific chimeric antigen receptor includes anti-CD32a single-chain antibody and anti-HIV gp120 single-chain antibody The coding nucleotide sequence of the anti-CD32a single-chain antibody and the coding nucleotide sequence of the anti-HIV gp120 single-chain antibody are connected in series in the bispecific chimeric antigen receptor coding gene. The bispecific chimeric antigen of the present invention targets the fusion protein gp120, a marker for HIV replication and proliferation, and the membrane protein CD32a, a marker for latency, to design dual-target CAR-T cells, which can completely eliminate HIV-infected target cells, It has great clinical promotion value.

The present invention relates to a new use of a medicine for improving Dicer expression. The medicament is used in the preparation of medicaments for preventing and treating colitis or colon cancer. The colitis is selected from inflammatory bowel disease, which is ulcerative colitis and Crohn's disease, and the colon cancer is colitis-associated colon cancer. The drug that increases the expression of Dicer is selected from anastrozole, its salts, its metabolites, and its derivatives.

The invention discloses an ultramicro powder epimedium constant-temperature preparation method. The preparation method comprises the following steps of: taking the traditional Chinese medicine of epimedium, cleaning, and drying until the content of moisture is less than or equal to 10%; milling the epimedium under the room temperature to obtain coarse powder with the particle size of 60-80 meshes; placing the obtained coarse powder in a rod mill to be milled to enter a dedicated bidirectional air flow screening machine through the air to be subject to 500-mesh screening, obtaining powder with the particle size being less than or equal to 25 microns; and transmitting the powder into a cyclone collector through the air flow produced by a draught fan to obtain the finished product of the ultramicro powder epimedium, wherein the powder which is not subject to the 500-mesh screening is collected by a funnel, and is transmitted into the rod mill through a pipeline to be circulated and milled again. The invention has the advantages that the whole preparation process is carried out under the room temperature, no low temperature or special additional condition is needed, the prepared finished product of the ultramicro powder epimedium has the particle size being less than or equal to 25 microns, the cell wall-broken rate and the bioavailability are greatly improved, and the pharmacological effect of the medicine is strengthened. By the bidirectional air flow screening machine used in the invention, the preparation process of the ultramicro powder epimedium is simplified.

The invention relates to a method of general-purpose primer PCR that is an immune catching method for detecting bacteria. Coating is carried out based on idiosyncratic antibody of various bacteria. With it being closed, the coated antibody is added into the bacteria solution at 36-38 deg.c for immune catching. Then heat denaturation releases template, and cracking liquid is extracted as PCR reaction template. Finally, using general-purpose primer of bacteria gene 16S rRNA to carry out PCR. The invention combines specificity of antigen-antibody with PCR, which has powerful rate of increasing production, to build iUPPCR for detecting pathogenic bacteria. The invention can catch idiosyncratic bacteria and increase caught bacteria so as to reach the goal for rapid detecting idiosyncratic bacteria. The invention possesses features of good repetitiveness, high sensibility and better specificity. It can detect almost any bacterium.

The invention relates to a gene chip, and discloses an integrated detection gene chip of an mtDNA mutation site related to Leber genetic optic neuropathy, as well as the preparation and the application thereof. A specific oligonucleotide detecting probe of the mtDNA mutational site related to the Leber genetic optic neuropathy and a specific oligonucleotide detecting probe of a mononucleotide polymorphyism site mtDNA 11719G>A are fixed on the carrier surface lattice of the gene chip. The gene chip has the advantages of time saving, low cost, simple operation and the like, has 32 mutation sites related to the Leber genetic optic neuropathy in a specific detecting clinical sample only through one-time PCR amplification and one-time crossing reaction, and is suitable for the gene diagnosis of the Leber genetic optic neuropathy.

The invention relates to a mutation site examination of mitochondrial diseases MELAS and MERRF syndrome correlated mtDNA, disclosing a mutation site detection gene chip correlated with the syndrome mtDNA of the mitochondrial diseases MELAS and MERRF, wherein, a specific oligonucleotide probe is fixed on the surface lattice of the gene chip carrier and is used for detecting the mtDNA mutation sites correlated with mitochondrial diseases syndrome MELAS and MERRF. The invention also further discloses a preparation and application method of the gene chip. The gene chip of the invention can be used for detecting all mutation sites mtDNA concerned with the mitochondrial disease syndrome MELAS and MERRF with high sensibility and specificity and good repeatability; compared with the traditional molecular biological methods, the testing time is greatly shortened, and the purposes of clinical diagnosis and identification diagnosis of the MELAS and MERRF syndrome are realized.