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Detection method of pdl1 gene in peripheral blood circulating tumor cells of patients with non-small cell lung cancer

A technology for non-small cell lung cancer and peripheral blood circulation, applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as inability to detect PDL1 in patients, achieve the effects of reducing detection procedures and costs, improving detection efficiency, and good clinical promotion value

Active Publication Date: 2019-07-05
上海立闻生物科技有限公司
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Problems solved by technology

The present invention separates and detects the circulating tumor cells of the patient, thereby realizing the evaluation of the expression of the patient's PDL1, and solving the problem that the patient's PDL1 cannot be detected due to the inability to obtain tumor pathological tissue samples. All patients who obtain tumor pathological tissue through surgery and other means benefit

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  • Detection method of pdl1 gene in peripheral blood circulating tumor cells of patients with non-small cell lung cancer
  • Detection method of pdl1 gene in peripheral blood circulating tumor cells of patients with non-small cell lung cancer
  • Detection method of pdl1 gene in peripheral blood circulating tumor cells of patients with non-small cell lung cancer

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[0055] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0056] In the present invention, the term HE staining hematoxylin-eosin staining (hematoxylin-eosin staining) is a commonly used staining method in paraffin section technique. Hematoxylin staining solution is alkaline, which mainly makes the chromatin in the nucleus and ribosomes in the cytoplasm purple-blue; eosin is an acidic dye, which mainly makes the components in the cytoplasm and extracellular matrix red.

[0057] 1. Separation and acquisition of circulating tumor cells from the peripheral blood of patients with non-small cell lung cancer by using a membrane filtration device:

[0058] Obtaining blood samples from patients with non-small cell lung cancer:

[0059] The peripheral blood of 5 patients diagnosed with non-small cell lung cancer was collected, and 5ml of peripheral blood was collected from each case. At the same time, 5 ml of blood s...

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Abstract

The invention provides a detection method for a non-small cell lung cancer peripheral blood circulating tumor cell PDL1 gene. The method comprises the steps that a, a peripheral blood sample from a non-small cell lung cancer is subjected to membrane filtration, and peripheral blood circulating tumor cells are obtained; b, a filter membrane obtained in the step a is fixed; c, the filter membrane obtained in the step b is detected in situ to determine the PDL1 expression condition; if PDL1 monochrome positive cells are detected in the step c, and HE dyeing is further used for identifying circulating tumor cells for the filter membrane.

Description

technical field [0001] The invention relates to a method for detecting a new target protein of peripheral blood circulating tumor cells, in particular to a method for detecting the PDL1 gene of peripheral blood tumor cells of patients with non-small cell lung cancer. Background technique [0002] Programmed cell death 1 ligand 1 (PDL1), also known as surface antigen differentiation cluster 274 (cluster of differentiation 274, CD274) or B7 homolog (B7 homolog 1, B7-H1), is A protein in humans, encoded by the CD274 gene. Non-small cell lung cancer induces the formation of an immunosuppressive tumor microenvironment by expressing PDL1, evading the body's anti-tumor immune response, and preventing tumor cells from being monitored and eliminated by the body's immune system. Studies have shown that there is a significant correlation between the expression level of PDL1 / PD1 in non-small cell lung cancer tissues and the clinicopathological factors and prognosis of patients. PDL1 / P...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574G01N33/533
CPCG01N33/533G01N33/57496
Inventor 闻丹忆
Owner 上海立闻生物科技有限公司
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