Screening method for serum (plasma) antibody based on membrane structure and preparation method of screening and detecting kit

A detection kit and membrane structure technology, which is applied in the field of blood type determination and detection, can solve problems such as limited application, wrong judgment, and long time consumption, and achieve good clinical promotion value, short detection time, and objective results

Inactive Publication Date: 2015-09-30
TIANJIN DEXIANG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The plate method and column agglutination method are complex to operate; conditions such as centrifugation will affect the experimental results; it is easy to cause misjudgment when there are many samples; auxiliary equipment is required and time-consuming, which limits the application in emergency situations

Method used

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  • Screening method for serum (plasma) antibody based on membrane structure and preparation method of screening and detecting kit
  • Screening method for serum (plasma) antibody based on membrane structure and preparation method of screening and detecting kit
  • Screening method for serum (plasma) antibody based on membrane structure and preparation method of screening and detecting kit

Examples

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Embodiment example 1

[0023] A method for screening and detecting antibodies in serum or plasma based on filter membrane structure

[0024] In this method, a filter membrane that allows a single red blood cell to pass through is selected as the core material, and cells, cell fragments, or purified antigens are bound to the filter membrane, and the filter membrane is surrounded by water-absorbing materials. On the surface of the filter membrane, after incubation for a certain period of time, after washing with a small amount of washing solution, specific indicator cells are added dropwise to the surface of the filter membrane, and finally rinsed with the washing solution. When the antigen on the membrane specifically binds to the antibody in the serum (plasma), it can undergo a cell agglutination reaction with the specific indicator cells, and then form a red mass visible to the naked eye. When the antigen on the membrane is not combined with the antibody in the serum (plasma), the indicator cells w...

Embodiment example 2

[0027] Preparation and detection steps of irregular antibody screening and detection kit in serum or plasma

[0028] 1 Immobilize blood group antigens on filter membrane

[0029] Preparation of blood group antigen: select three groups of O-type erythrocytes with known antigen profiles and a group of O-type erythrocytes sensitized by IgG as the detection antigen group and positive control group respectively, use low osmotic pressure solution to break the cells, centrifuge to remove the supernatant, The precipitate was then washed with the same low osmotic pressure solution until the supernatant was colorless and transparent, and the red blood cell membrane precipitate was collected as the blood group antigen extract. Add antigen protecting agent to dilute antigen extract. Antigen protection agent can be 5mM phosphate buffer saline (pH7.4) containing 5% bovine serum albumin, 5% sucrose, 5% PVP, 5% calf serum. The antigen protection solution can protect the antigenicity and enh...

Embodiment example 3

[0039] Preparation and detection method of platelet antibody screening detection kit

[0040] 1 Immobilize platelet antigens on filter membrane

[0041] After chemically treating the activated filter membrane, platelet antigen (or platelets) diluted with a protective agent was added dropwise to the center of the 1.5cm×1.5cm filter membrane, then dried at 37°C and sealed for storage.

[0042] 3 Assembly of the detection reagent card:

[0043] The detection reagent card consists of processed filter membrane, water-absorbing material and plastic support. The plastic bracket consists of three parts, one is the cover, the other is the sample plate, and the third is the suction box. The sample loading plate is a square plastic plate with 4 holes (0.5 cm in diameter), which can be removed from the reagent card. The filter membrane with platelet antigen is pasted on the back of the sample loading plate, and the antigen center on the filter membrane corresponds to the sample hole on...

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Abstract

The invention discloses a screening method for a serum (plasma) antibody based on a membrane structure and a preparation method of a screening and detecting kit. The screening method is characterized by comprising the steps of with a filter membrane capable of allowing single erythrocyte to pass through as a core material, combining the erythrocyte, a cell debris or a purified antigen onto the filter membrane; enclosing water absorbent materials at the periphery of the filter membrane; during detecting, dropwise adding serum or plasma to be detected onto the surface of the filter membrane; after incubating for a period of time, washing with a trace amount of washing liquid; then, dropwise adding a specific indicator cell onto the surface of the filter membrane; and finally, washing with a washing liquid. The screening method has the beneficial effects that the detecting time is short, and the result is objective.

Description

technical field [0001] The invention relates to a blood type determination and detection technology, in particular to a membrane structure-based serum (plasma) antibody screening method and the preparation of a screening and detection kit. Background technique [0002] In addition to conventional ABO blood group antibodies, serum or plasma also contains a large number of irregular antibodies and platelet antibodies. Clinically significant irregular antibodies can lead to hemolytic transfusion reactions, hemolytic disease of the newborn, etc. Therefore, in clinical testing, screening blood donors' serum or plasma for irregular antibodies can prevent blood containing irregular antibodies from being injected into patients, causing hemolytic transfusion reactions in patients; Antibody serum prepared from blood is beneficial to the detection of rare blood types; irregular antibody screening for patients who need blood transfusion is helpful for blood selection, which can prevent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/80G01N33/96
CPCG01N33/80
Inventor 黄志刚
Owner TIANJIN DEXIANG BIOTECHNOLOGY CO LTD
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