80 results about "Enterobacter aerogenes" patented technology

The invention discloses high-concentration chemical sewage composite inocula. The high-concentration chemical sewage composite inocula comprise, by weight, 180-220 parts of bacillus cereus, 150-160 parts of bacillus circulans, 75-85 parts of Exiguobacterium acetylicum, 90-120 parts of bacillus coagulans, 150-170 parts of pseudomonas aeruginosa, 20-25 parts of micrococcus luteus, 120-140 parts of thiobacillus thiooxidans, 180-230 parts of rhodococci, 20-30 parts of lactobacillus brevis, 50-65 parts of paracoccus denitrificans, 95-112 parts of alcaligenes faecalis, 110-125 parts of pseudomonas putida, 100-130 parts of enterobacter aerogenes, 280-320 parts of beer yeast and 140-160 parts of lactobacillus fermenti. All strains of the composite inocula reasonably cooperate with each other, produce synergistic effects, do not produce antagonism, have a high survival rate, is suitable for one step addition and fast forms dominant bacterial community. The high-concentration chemical sewage composite inocula have unique treatment effects on high-concentration chemical sewage, have a COD clearance rate of 94.17%, reduce an operation cost and promote qualified discharge.

The invention discloses an enterobacteria aerogenes and the application. The inventor screens Enterobacter aerogenes XM02 by a great deal of experimental researches. The bacterial has ferrum redactum active, facultative and electron donor, which can survive in the aerobic or anaerobic environment, wherein the electron donor in the anaerobic environment has the wide application, the electron donor which can electron donor comprises glycerin, citrate, dextrose, sucrose or the like, the ferric iron which can deacidize comprises szomolnokite, goethite, lepidocrocite, bloodstone, yellow copiapite or the like.

The invention relates to a compound microbial preparation capable of inhibiting and killing blue-green algae. The preparation is prepared from the following components by weight percent: 35%-60% of chicken manure extract, 35%-60% of rice husk powder, and 2%-5% of complex microbial inoculants, wherein the complex microbial inoculants comprise bacillus subtilis, thermoactinomyces, enterobacter aerogenes and saccharomycetes, the effective viable count of the bacillus subtilis is 1,900 million / gram, the effective viable count of the thermoactinomyces is 6 million / gram, the effective viable count of the enterobacter aerogenes is 1 million / gram, and the effective viable count of the saccharomycetes is 3-4 million / gram. The problems that a method for eliminating cyanobacterial bloom in the prior art consumes a lot of manpower and material resources and easily causes secondary pollution are solved.

The invention belongs to the field of examination of microorganisms, and relates to a method for quickly identifying bloodstream infection bacteria by a high-resolution fusion curve method. The method comprises the following steps of: performing smear gram staining on a blood culture positive culture flask to distinguish negative bacteria and positive bacteria; selectively amplifying different 16S fragments for the negative bacteria and the positive bacteria; and analyzing by the high-resolution fusion curve method. Common bloodstream infection pathogenic bacteria comprise staphylococcus aureus, staphylococcus epidermidis, enterococcus faecalis, enterococcus faecium, Escherichia coli, Klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, enterobacter cloacae, Serratia marcescens, Klebsiella oxytoca, acinetobacter lwoffii, enterobacter aerogenes, stenotrophomonas maltophilia and burkholderia cepacia. By the method, the common bloodstream infection pathogenic bacteria can be measured quickly, efficiently and accurately, so that strong evidences are provided and precious time is striven for subsequent treatment.

The invention provides a method for treating bacterial infections. In one aspect, the invention comprises orally administering a pharmaceutical composition to an animal, wherein the composition comprises a pharmaceutically acceptable excipient and an antibacterial effective amount of negamycin, or a pharmaceutically acceptable salt, prodrug or isomer thereof. An aspect of the invention also relates to a method of treating a bacterial infection, wherein the method comprises intravenously administering a pharmaceutical composition to an animal, and wherein the composition comprises a pharmaceutically acceptable excipient and an antibacterial effective amount of deoxynegamycin, or a pharmaceutically acceptable salt, prodrug or isomer thereof. An aspect of the invention also relates to a method of treating a bacterial infection, wherein the method comprises administering to an animal an antibacterial effective amount of negamycin or deoxynegamycin, or a pharmaceutically acceptable salt, prodrug or isomer thereof, and wherein the infecting bacteria are selected from a group of bacteria consisting of the following: Acinetobacter baumanii, Citrobacter freundii, Enterobacter aerogenes, haemophilus influenzae, Moraxella catarrhalis, Staphylococcus aureus MRSA, Staphylococcus aureus GISA, Staphylococcus epidermis, Streptococcus pneumoniae PenR, Streptococcus pneumoniae PenS and Streptococcus pyogenes.

The invention discloses a method for acquiring microbes capable of degrading octachlorodipropyl ether from soil or sludge, and a high-efficiency octachlorodipropyl ether-degrading bacterium According to the method, sludge or soil containing octachlorodipropyl ether and acquired from a petrochemical factory, a mosquito-repellent incense factory, farmland or other places is used as a sample, and culture of a microbial strain, separating, purifying, domestication and screening are successively carried out so as to select out the octachlorodipropyl ether-degrading bacterium. The method provided by the invention extracts from soil and sludge an Enterobacter aerogenes strain M2016517 with high octachlorodipropyl ether degradability, and the Enterobacter aerogenes strain M2016517 is preserved in Guangdong Microbiological Culture Collection Center on May 17, 2016, with an accession number of GDMCC No. 60041. The octachlorodipropyl ether degradation rate of the strain is as high as 95%. The method provided by the invention can achieve the purposes of screening and domestication of the strain at the same time, so the domestication period is obviously shortened; moreover, the method is simple, convenient and efficient, and the screened octachlorodipropyl ether-degrading bacterium has high octachlorodipropyl ether degradation rate.

The invention provides application of a microbe strain-Enterobacter aerogenes capable of degrading various pyrethroid pesticides simultaneously to the degrading of pyrethroid pesticide residue, and a preparation for degrading the pyrethroid pesticide residue prepared by the strain. An efficient degrading strain is obtained through screening to simultaneously degrade three pyrethroid pesticide residues, namely bifenthrin, fenpropathrin and cypermethrin, and is analyzed and researched in degrading characteristic, so as to disclose the basic rule of the strain for degrading the pesticide and provide scientific basis for controlling pyrethroid pesticide residue.

The invention discloses a molecular beacon and a kit for quickly identifying various clinically common bacteria and belongs to the technical field of microbiological detection. The sequences SEQ ID of the molecular beacon are as shown in v6p1, v6p2, v1p1 and v1r. Through comparison between various clinically common bacteria 16s ribosome RNA sequences in an NCBI gene sequence database and design of the molecular beacon at a tag sequence, clinically common 18 bacteria, including baumanii, A.hydrophila, burkholderia cepacia, citrobacter freundii, enterobacter cloacae, enterococcus faecium, enterococcus faecalis, enterobacter aerogenes, escherichia coli, klebsiella pneumoniae, listeria monocytogenes, proteus mirabilis, pseudomonas aeruginosa, staphylococcus epidermidis, staphylococcus aureus, salmonella, serratia marcescens and stenotrophomonas maltophilia, can be quickly identified. The invention further discloses the kit containing the molecular beacon and used for detecting various bacteria, and the kit can quickly and accurately identify various clinically common bacteria.

The invention discloses a gene chip for detecting 15 clinical common pathogenic microorganisms. The gene chip comprises specific detection probes fixed on a solid phase vector, wherein the probes are designed for 16SrRNA gene and ITS gene sequences of staphylococcus aureus, klebsiella pneumoniae, pseudomonas aeruginosa, proteus mirabilis, Escherichia coli, shigella, enterobacter aerogenes, pseudomonas fluorescens, candida albicans, candida glabrata, candida tropicalis, candida parapsilosis, aspergillus terreus, aspergillus flavus and rhizopus oryzae, and screened and verified by repeated tests, and have high hybridization specificity and accuracy. The chip provided by the invention ensures simple detection operations and relatively lower cost, saves time, is applied to the screening of the clinical common pathogenic microorganisms, the early rapid diagnosis of critical patients and the diagnosis of suddenly occurring infection event pathogens and favorable for clinically rapidly diagnosing infection sources and functions in the early detection and early diagnosis of clinical diseases.

Described herein are antimicrobial articles having improved antimicrobial efficacy and antireflective properties. Further described are methods of making and using the improved articles. The antimicrobial articles generally include an antimicrobial element and an antireflective element. The antireflective element, in some cases, can be disposed directly on a glass, glass-ceramic or ceramic substrate and the antimicrobial element is disposed on the antireflective element. The article can exhibit a reflectance of about 4% or less (and less than 1% in some cases) in the range of about 425 nm to about 725 nm. Further, the article can be characterized with an antimicrobial efficacy by exhibiting at least a 2 log reduction in a concentration of at least Staphylococcus aureus, Enterobacter aerogenes, and Pseudomonas aeruginosa bacteria under a Modified EPA Copper Test Protocol.

A compound method of high-yield nucleoside phosphorylase strains and arabinose nucleoside pertains to biochemical engineering field, in particular to the high-yield nucleoside phosphorylase strains and a method for compounding arabinose purine nucleoside with the strains by an enzyme method. The invention aims at solving a technical problem for providing the strains of the high-yield nucleoside phosphorylase and strains of uridine phosphorylase and the method for producing the arabinose purine nucleoside with the strains. The invention discloses enterobacter aerogenes with a preservation number of CGMCC No.2035 and the method for producing the arabinose purine nucleoside with the strains, and the invention comprises steps that (1) the enterobacter aerogenes DWOQ-58 of the invention is cultured and collected, and (2) the enterobacter aerogenes DWOQ-58 is contacted with arabinose donor and receptors of purine base. The strains of the invention are rich in vigor and resists 5-flucytosine with an average conversion rate of more than 80 percent in general and the reaction time of the invention is shortened to less than 12 hours.

The invention discloses a kit for detecting clinically common pathogenic bacteria by adopting an RNA isothermal amplification melting curve method and applications of the kit, belonging to the technical field of biological detection. The kit can detect the 16S rRNA of the following 16 clinically common pathogenic bacteria: staphylococcus aureus, pseudomonas aeruginosa, klebsiella pneumoniae, escherichia coli, proteus mirabilis, enterobacter aerogenes, pseudomonas fluorescens, acinetobacter baumannii, salmonella typhimurium, enterobacter cloacae, enterococcus faecium, enterococcus faecalis, bacillus proteus vulgaris, staphylococcus epidermidis, onion klebsiella and stenotrophomonas maltophilia. The kit has the features of high specificity, high sensitivity, low pollution, and detection accuracy and rapidness, plays an important role in clinical rapid identification and detection analysis of microbes, and has the wide application prospect.

The invention discloses an agricultural compound microbial agent and application thereof, belonging to the technical field of microorganisms. The agricultural compound microbial agent comprises enterobacter aerogenes CT-B09-2, acinetobacter calcoaceticus WYS-A01-1, bacillus amyloliquefaciens JL-B05, bacillus amyloliquefaciens JL-B06 and bacillus licheniformis LYN-3, wherein the total viable bacteria concentration is (5-8)*10<8> cfu / ml or (2-3)*10<9> cfu / g. The agricultural compound microbial agent can promote the growth of crops, improves the disease-preventing, disease-resisting, stress-resisting and continuous-cropping-resisting capabilities of the crops, increases the yield of the crops and improves the quality of agricultural products.

The invention relates to an Aerobacter enterobacteria which can excrete algaelysing substances and its application in controlling blue-algae water bloom. The said strain is a bacteria which can excrete algaelysing substances and is obtained by thickening the natural water sample 10-100 times to perform algaelysing experiment separation, which is thought as Aerobacter enterobacteria W5 by biochemical determination. The white powder preparation can be obtained after the fermentation by the spray drying of Aerobacter enterobacteria, and the algaelysing substances all can effectively kill the water bloom blue algae in an application concentration of 2-200ppm. Furthermore, the said preparation is of convenient use, is safety and nontoxic, and is no harm to the environment. The condition of fermentation of the said Aerobacter enterobacteria is: the sterilizing parameter is 121 DEG C and 20-30 minutes; the fermentation parameter is: the pH is 5.8.0-7.0 and DO is 70-90, the fermentation temperature is 34.0-39.0 DEG C, the mixing speed id 70-130 r / minute, and the fermentation time is 16-48 minutes. The fermentation culture medium formulation is: in the fermentation process there is no need to supplement the materials and to adjust the PH, and the spray drying condition of the fermentation products is that the import temperature is 198-210 DEG C and the export temperature is 68-79 DEG C.

The invention provides a gene chip for detecting common pathogenic bacteria in cosmetics. The gene chip comprises a solid phase carrier and an oligonucleotide probe fixed on the solid phase carrier, wherein the oligonucleotide probe comprises DNA segments which are selected from 16S-23SrDNA intermediate zones of micrococcus luteus, staphylococcus aureus, streptococcus pyogenes, salmonella, proteus mirabilis, citrobacter freundii, pseudomonas aeruginosa, enterobacter aerogenes and providencia stuartii and 18S-28SrDNA intermediate zones of the ipaH gene of shigella, candida albicans and cryptococcus neoformans, or complementary DNA or RNA sequences of the DNA segments. The invention also provides a kit for detecting the common pathogenic bacteria in the cosmetics by use of the gene chip. The gene chip and the kit can be used for detecting the common pathogenic bacteria in the cosmetics, and are simple and convenient to operate, high in accuracy and excellent in repeatability.

The invention discloses an Enterobacter aerogenes S27 for degrading malachite green. The Enterobacter aerogenes with the preservation number CCTCC NO: M 2017452 is preserved on Aug. 28, 2017 in ChinaCenter For Type Culture Collection in Wuhan University in Wuhan province of China. The Enterobacter aerogenes resists salt, is able to adapt to harsh environment, is low-cost in use, can recover ecological balance, and is applicable to degradation of the malachite green in wastewater, thereby serving as a beneficial strain resource for environment pollution caused by the malachite green.

The present invention provides phyllostachys edulis endophytic Enterobacter aerogenes with phosphorus-dissolving, potassium-dissolving and nitrogen-fixation effects and application thereof. The phyllostachys edulis endophytic Enterobacter aerogenes is Enterobacter aerogenes CT-B09-2 which is preserved in a general microorganism center of the China committee for culture collection of microorganismson July 3, 2017, and the preservation number is CGMCC No.14377. The phyllostachys edulis endophytic Enterobacter aerogenes has the phosphorus-dissolving, potassium-dissolving and nitrogen-fixation effects, can be colonized in plants and improve the enzyme activity, and has the important effects on regulating plant growth and development.

The invention belongs to the field of quality control of microbiological detection, and particularly relates to a sample for verifying capacity of total coliform flora in domestic drinking water and a preparation method thereof. The sample for verifying the capacity of the total coliform flora in the domestic drinking water comprises target flora and background flora, wherein the target flora consists of escherichia coli, klebsiella pneumoniae, enterobacter cloacae, proteus mirabilis and enterobacter aerogenes; the background flora consists of staphylococcus aureus and bacillus cereus. The uniformity and the stability of the sample meet capacity verifying requirements, so that the stability of a test sample can be ensured to the maximum extent, and even during transportation and storage, change of the escherichia coli content does not affect the total amount of the total coliform flora in the statistical sense; the preparation method of the sample is simple in process and high in successful rate.

The invention discloses a spiro quinazoline derivative and a preparation method thereof. The molecular structural formula of the spiro quinazoline derivative is as shown in the specification, wherein R1 is any one or two out of hydrogen, methyl, trifluoromethyl, fluorine or chlorine, and R2 is hydrogen, methyl or phenyl. The spiro quinazoline derivative has an excellent antibacterial effect particularly on pseudomonas aeruginosa, enterobacter aerogenes and staphylococcus epidermidis, and has the better antibacterial effect on the pseudomonas aeruginosa, the enterobacter aerogenes and the staphylococcus epidermidis than a marketed bactericide bromogeramine, and the preparation method has the advantages of simple process, mild condition, high productivity, simple and convenient post treatment, high activity of an obtained product and the like.

The invention provides sika deer coronet antibacterial peptides / proteins with antibacterial effect and applications thereof, including sika deer coronet micromolecular polypeptides, sika deer coronet collagen hydrolyzing peptides, and sika deer coronet micromolecular protein monomers. The peptides / proteins are subjected to inhibition ring tests so as to observe the inhibiting effect on Gram-negative bacteria, Gram-positive bacteria, and fungi; wherein the Gram-negative bacteria comprise enterobacter aerogenes, proteus mirabilis, pseudomonas aeruginosa, and escherichia coli. The Gram-positive bacteria comprise staphylococcus lentus, lactococcus garvieae, staphylococcus xylosus, bacillus subtilis, and staphylococcus intermedius. The fungi are candida albicans. Under the effect of various bacteriostatic agents, the enterobacter aerogenes, bacillus subtilis, escherichia coli, pseudomonas aeruginosa, staphylococcus intermedius, and staphylococcus xylosus are inhibited to a certain degree, and the candida albicans, lactococcus garvieae, proteus mirabilis, and staphylococcus lentus are not inhibited.

The invention provides a compound microbial agent for reducing ammonia gas release in chicken manure composting and a preparation method and application thereof, and belongs to the technical field ofmicroorganisms. The compound microbial agent consists of Pantoea agglomerans HAAS-1, Anoxybacillus rupiensis HAAS-2 and Enterobacter aerogenes HAAS-3. The compound microbial agent can effectively reduce the ammonia gas yield in the chicken manure composting process, and when the compound microbial agent is added in an amount of 2.5% of the composting material weight, the total release amount of ammonia gas can be reduced by 65% or above in the first 15 days of composting fermentation; and the compound microbial agent is used for ammonia gas removal of the chicken manure compost, and is simpleto use, low in cost and free from secondary pollution.

The invention discloses a recombinant plasmid for eliminating industrial wastewater mercury pollution, a construction method, a recombinant engineering bacterium and an application. The recombinant plasmid is established by inserting tobacco chloroplast rrn16 gene strong promoter, ribosome binding site conserved sequence of 5'UTR sequence of escherichia coli T7 phage gene 10, 3'-end non-coding area of tobacco chloroplast rps16 gene, enterobacter aerogenes polyphosphate kinase gene ppk and pseudomonas K-62 strain on the basis of plasmid pET28a and by eliminating mer operon of merA and merG. Organic mercury and inorganic mercury in wastewater are transferred into bacterial cells through merT-merP, the organic mercury is degraded into divalent mercury through merB1 and merB2, the divalent mercury is chelated into the cells through ppk, the toxicity of mercury to the bacteria cells is reduced, mercury in the wastewater is accumulated inside the bacterial cells, and mercury pollution in the waster is eliminated through collecting the recombinant engineering bacteria.

The invention discloses hydrogen-producing engineering bacteria and application thereof. The hydrogen-producing engineering bacteria are engineering bacteria obtained by inactivating delta hycA protein in E.aerogenes IAM1183, wherein the amino acid sequence of the delta hycA protein is sequence 1 in a sequence list. The hydrogen-producing engineering bacteria can be applied to biological hydrogen production and are of great directive significance to hydrogen production by means of enterobacter aerogenes. Moreover, the hydrogen-producing engineering bacteria have the advantages of strong environmental adaptability, wide substrate utilization range, strong substrate utilization capability and high hydrogen-producing efficiency.

The invention provides a growth-promoting composite endophytic bacterial agent and belongs to the technical field of microorganisms. The growth-promoting composite endophytic bacterial agent comprisesenterobacter aerogenes CT-B09-2, acinetobacter calcoaceticus WYS-A01-1, bacillus amyloliquefaciens JL-B05 and bacillus amyloliquefaciens JL-B06, wherein the mass ratio of the enterobacter aerogenes CT-B09-2 to the acinetobacter calcoaceticus WYS-A01-1 to the bacillus amyloliquefaciens JL-B05 to the bacillus amyloliquefaciens JL-B06 is (3 to 5):(3 to 6):(3 to 6):(3 to 8); and the total viable bacterium concentration in the composite endophytic bacterial agent is (5 to 8)*10<8> cfu / ml or (2 to 3)*10<9> cfu / g. The growth-promoting composite endophytic bacterial agent has the advantages that thestrain has strong phosphorus-dissolving potassium-dissolving and nitrogen-fixing effects; colonization in the plant bodies can be realized; the phyllostachys edulis photosynthetic characteristics andthe biological enzyme activity are further improved; and important effects are achieved on regulating the growth and development of the phyllostachys edulis.

The invention provides Enterobacter aerogenes M6R9 which can degrade pyrethroid pesticide residues, in particular can degrade the residues of three pyrethroid pesticides at the same time, namely bifenthrin, fenpropathrin and cypermethrin. The strain is preserved in the China General Microbiological Culture Collection Center (address: Institute of Microbiology of Chinese Academy of Sciences, Building 3, No. 1 Precincts, Beichen West Road, Chaoyang District, Beijing, 100101), the preservation number of the strain is CGMCC No. 4155, and the conservation date is September 8, 2010. The beneficial effects of the Enterobacter aerogenes M6R9 in the invention are mainly embodied as follows: the invention provides a new strain which can degrade the residues of bifenthrin, fenpropathrin and cypermethrin at the same time and provides the research basis for controlling the pyrethroid pesticide residues in agricultural products, and the strain has wide application prospect.

The invention provides a primer group for detecting escherichia coli O157 in feed. The primer group comprises an inner primer FIP, an inner primer BIP, an outer primer F3, an outer primer B3 and a loop primer LB. A method of detecting the escherichia coli O157 in the feed by using the primer group comprises the steps of escherichia coli O157 culture, DNA (deoxyribonucleic acid) extraction, loop-mediated isothermal amplification reaction and result observation. Tests show that the primer group has no specific expression for enterobacter aerogenes, escherichia coli and salmonella enteritidis, and obvious expression and high specificity for the escherichia coli O157; the feed is detected by a loop-mediated isothermal amplification detection method; a detection result is very precise; and a detection limit of the escherichia coli O157 in the feed is 2 CFU (colony forming unit) / 25g. In addition, the primer group has the advantages of high detection speed, convenience in operation and the like for the detection of the feed.

An antimicrobial composition including an alkyl pyridinium or a salt of alkyl pyridinium and an isothiazolinone or a salt of an isothiazolinone is effective against bacterial and fungicidal growth and resulting degradation in paints, emulsions, adhesives, and joint compounds, among other things. The composition may be employed in an aqueous liquid medium. Carriers may be employed to deliver the antimicrobial mixtures in liquid or pellet form. The invention is also a method for inhibiting microbial growth which employs the antimicrobial composition.In a preferred aspect, the invention is antimicrobial composition which includes cetyl pyiridinium choride and benzisothiazolinone salt, in a proportion that is synergistically effective against Pseudomonas aeruginosa, Escherichia coli, Enterobacter aerogenes, and Alcaligenes faecailis.

The invention provides a microbial agent combining enterobacter aerogenes and klebsiella aerogenes, the microbial agent is used for treating lead and cadmium polluted soil, and the microbial agent comprises enterobacter aerogenes and klebsiella aerogenes; the enterobacter aerogenes is Enterobacter aerogenes W6, the Latin classification name of the enterobacter aerogenes is Enterobacter aerogenes, the enterobacter aerogenes is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is July 13, 2021, and the preservation number is CGMCC No.22888. The enterobacter aerogenes can be used for preparing the feed additive, and the feed additive can be used for preparing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive. The Klebsiella aerogenes are Klebsiella aerogenes Wn, the classification name of the Klebsiella aerogenes is Klebsiella aerogenes Wn, the Klebsiella aerogenes Wn is the Klebsiella aerogenes Wn, the Klebsiella aerogenes Wn is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is July 13, 2021, and the preservation number is CGMCC No. 22889.

The invention provides a method for producing hydrogen by utilizing facultative anaerobe fermentation, and in particular relates to two aspects of strain screening and fermentation condition optimization. The screened facultative anaerobe is Enterobacter aerogenes CICC10293; through tests, the optimal fermentation process conditions of a strain are as follows: a culture medium is concretely prepared from 4 to 6g / L of peptone, 2 to 4g / L of beef extract, 4 to 6g / L of NaCl, 1 to 1.5g / L of K2HPO4 and 0.1 to 0.2g / L of FeSO4.4H2O; a fermentation substrate is dextrose, and the concentration is 20g / l; fermentation adopts a mode of batch fermentation; constant temperature shaking culture is carried out under conditions that the pH is 6.0 to 7.0 and the temperature is 40 DEG C, and the shaking frequency is 170r / min; and the culture time is about 20 hours until hydrogen production stops. The hydrogen yield can reach 134mLH2 / 100mL of liquid culture medium, the hydrogen production efficiency is high, the requirement for fermentation environment conditions is low, and the invention has broad application aspect.

The invention relates to an enterobacter aerogenesa ZJB-17003 and application thereof. An enantio-selectivity value of the enterobacter aerogenesa ZJB-17003 for catalyzing N-phenylacetyl-DL-amino acidto prepare L-amino acid reaches 99% and an enantio-selectivity value for catalyzing 2-N-phenylacetyl-4-[hydroxy(methyl)phosphoryl]butyric acid to prepare 2-amino-4-[hydroxy(methyl)phosphoryl]butyricacid reaches 99%.