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Phyllostachys edulis endophytic Enterobacter aerogenes with phosphorus-dissolving, potassium-dissolving and nitrogen-fixation effects and application thereof

A technology of Enterobacter aerogenes and Phyllostachys pubescens, applied in the field of microorganisms, to achieve the effect of increasing chlorophyll content and good strain resources

Active Publication Date: 2018-09-28
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports on the isolation of culturable endophytic bacteria from different tissues of moso bamboo, the screening of endophytic bacteria with phosphorus, potassium, and nitrogen fixation functions, and the functional endophytic bacteria that can promote photosynthesis.

Method used

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  • Phyllostachys edulis endophytic Enterobacter aerogenes with phosphorus-dissolving, potassium-dissolving and nitrogen-fixation effects and application thereof
  • Phyllostachys edulis endophytic Enterobacter aerogenes with phosphorus-dissolving, potassium-dissolving and nitrogen-fixation effects and application thereof
  • Phyllostachys edulis endophytic Enterobacter aerogenes with phosphorus-dissolving, potassium-dissolving and nitrogen-fixation effects and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Screening of Enterobacter aerogenes in Phyllolytic Phosphorus, Potassium, and Nitrogen Fixing in Moso bamboo

[0025] (1) Screening process:

[0026] By gradient dilution method, 82 isolates were isolated from Moso bamboo central production areas of Wuyishan City, Changting County and Jiangle County, Fujian Province. The isolated endophytic bacteria were purified by the three-section line method, and whether the strain was purified was judged by microscopic examination. The purified bacteria were numbered, and single colonies were picked and transferred to the NA slope for future use. Through the determination of phosphorus, potassium, and nitrogen fixation effects on the plate, 20 endophytic bacteria strains with good phosphorus, potassium, and nitrogen fixation effects were initially screened out, and one endophytic bacteria with good phosphorus, potassium, and nitrogen fixation effects was finally screened out, labeled CT -B09-2.

[0027] (2) Colony chara...

Embodiment 2

[0054] Embodiment 2 Pot experiment

[0055] Enterobacter aerogenes ( Enterobacter aerogenes ) CT-B09-2 was activated on the NA slant, and a ring was picked and inserted into the NA medium (3g of beef extract, 10g of peptone, 5g of Nacl, 18g of agar, 1000ml of water, pH7.0-7.2), at 28℃, 180 rpm -1 Under the condition of shaking culture for 72 h, the culture solution was diluted with sterile water to make 1×10 8 cfu / mL bacterial suspension for inoculation. The net photosynthetic rate, transpiration rate, stomatal conductance and intercellular CO 2 Determination of photosynthesis indicators such as concentration.

[0056] The photosynthetic rate (Pn), transpiration rate (Tr), stomatal conductance (Gs) and intercellular CO of bamboo leaves were measured 15 days and 30 days after potted seedlings of Moso bamboo were inoculated with endophytic bacteria CT-B09-2. 2 Concentration (Gs). The results showed that the photosynthetic rate (Pn), transpiration rate (Tr) and stomatal ...

Embodiment 3

[0070] Embodiment 3 field experiment

[0071] Select the moso bamboo forest base in Longqishan Nature Reserve, Jiangle County, Fujian Province, select the moso bamboo of degree II, first use an electric drill to drill holes in the bamboo stems about 30cm away from the soil surface, and then take 30 mL of the above bacterial suspension and inject them with sterile syringes To the inside of the bamboo cavity of Moso bamboo, repeat inoculation with 30 mL the next day, and seal the cavity of the bamboo cavity with soil. 10 plants per treatment were repeated 3 times, and water was used as the control. 15d and 30d after treatment, 5-10 leaves of Moso bamboo in the four directions of east, south, west and north were collected by high branch shears respectively, and fully mixed as a mixed sample for chlorophyll content, catalase (CAT ), malondialdehyde (MDA), peroxidase (POD), superoxide dismutase (SOD), soluble protein content, soluble sugar and other physiological and biochemical i...

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Abstract

The present invention provides phyllostachys edulis endophytic Enterobacter aerogenes with phosphorus-dissolving, potassium-dissolving and nitrogen-fixation effects and application thereof. The phyllostachys edulis endophytic Enterobacter aerogenes is Enterobacter aerogenes CT-B09-2 which is preserved in a general microorganism center of the China committee for culture collection of microorganismson July 3, 2017, and the preservation number is CGMCC No.14377. The phyllostachys edulis endophytic Enterobacter aerogenes has the phosphorus-dissolving, potassium-dissolving and nitrogen-fixation effects, can be colonized in plants and improve the enzyme activity, and has the important effects on regulating plant growth and development.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a Phosphorus-decomposing and Potassium-decomposing, Nitrogen-fixing Enterobacter aerogenes plant and its application. Background technique [0002] Plant endophytic bacteria refer to those bacteria that live in various tissues and organs of healthy plants during a certain stage or all stages of their life history, and have established a harmonious relationship with plants. Due to their ecological advantages, endophytic bacteria in plants can colonize and transmit in plants for a long time, and are not easily affected by environmental conditions. They have a wide range of biological effects on plant growth and development, resistance to diseases and adverse environments, and are very rare natural bacteria. Biological resources have good research and development potential in agricultural production. People have isolated a variety of endophytic bacteria from various pl...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P21/00C12R1/01
CPCA01N63/10C12N1/20C12N1/205C12R2001/01
Inventor 袁宗胜刘芳张国防谢宝贵
Owner FUJIAN AGRI & FORESTRY UNIV
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