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Enterobacter aerogenes and klebsiella aerogenes combined fungicide, composition and application

A Klebsiella, Enterobacter aerogenes technology, applied in bacteria, microorganisms, microorganism-based methods, etc., can solve the problems of poor soil treatment effectiveness and long-term stability, improve diversity, avoid anti-solubility , to promote the effect of microbial phosphorus solubilization

Active Publication Date: 2022-04-29
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The main purpose of the present invention is to provide a bacterial agent, composition and application of Enterobacter aerogenes combined with Klebsiella aerogenes, aiming to solve the poor effectiveness and long-term stability of soil treatment in the prior art question

Method used

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  • Enterobacter aerogenes and klebsiella aerogenes combined fungicide, composition and application
  • Enterobacter aerogenes and klebsiella aerogenes combined fungicide, composition and application
  • Enterobacter aerogenes and klebsiella aerogenes combined fungicide, composition and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Screening and identification of Enterobacter aerogenes W6

[0080] 1. Culture conditions: aerobic, temperature 30° C., initial pH = 7 (that is, the initial pH of the culture medium is 7); wherein, 150 rpm / min shaker culture is used for culture under liquid conditions.

[0081] 2. Medium preparation: liquid medium 1, liquid medium 2, solid medium 2.

[0082] Wherein, the preparation process of liquid medium 1 is: calculate the quality of solid component according to the capacity and solid-liquid concentration (25g / L) prepared on demand, then account for 40% tryptone, 20% yeast powder, 40 % sodium chloride was weighed, and after weighing, the volume was constant;

[0083] The preparation process of liquid culture medium 2 is: according to the capacity and solid-liquid concentration (20.55g / L) prepared on demand, calculate the quality of solid component, then account for 48.7% glucose, 24.3% magnesium chloride, 1.2% magnesium sulfate by accounting for solid component mass...

Embodiment 2

[0099] Prepare lead and cadmium mother liquors: weigh 1.5985g of lead nitrate and 20.317g of cadmium chloride and dissolve them in a beaker filled with 500ml of ultrapure water. Tick ​​marks, labeled for later use.

[0100] 1. The role of Enterobacter aerogenes W6 in the passivation of lead and cadmium

[0101] 1. Pick a single colony from the flat plate of the W6 strain and culture overnight in 20 mL of liquid medium 1 (same as Example 1), then transfer to 100 mL of liquid medium 1 for expansion.

[0102] 2. Observe the growth density of the W6 strain, and measure its growth curve with a UV spectrophotometer at a wavelength of 600nm to determine the logarithmic growth phase and stable phase OD of the W6 strain 600 =1 for subsequent experiments; W6 reached OD at 5.5 hours of culture 600 =1 for a stable period.

[0103] 4, lead cadmium solution experiment is set, add 1ml lead mother liquor and 1ml cadmium mother liquor respectively (add 1ml lead mother liquor and 1ml cadmium...

Embodiment 3

[0116] The liquid medium 1 used in this embodiment is equal to that in Example 1, and additionally added glucose and tricalcium phosphate equivalent to the liquid medium 2; the liquid medium 2 used in this embodiment is equal to that in Example 1; The initial pH of the medium used in this example was 7.

[0117] 1. Effects of different media on Enterobacter aerogenes W6

[0118] Take 1% of the W6 culture solution and put them into medium 1 and medium 2 respectively as different nutrient sources for cultivation, place on a shaker at a constant temperature of 30°C, 150rpm / min, shake and cultivate for one week, and at the same time point every day in sterile Take a 3ml sample on the operating table in a 5ml centrifuge tube, centrifuge at 8000rpm for 5 minutes to get the supernatant, and use the molybdenum antimony anti-colorimetric method to measure the content change of available phosphorus, and record the corresponding pH value.

[0119] After the measurement, the result displ...

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Abstract

The invention provides a microbial agent combining enterobacter aerogenes and klebsiella aerogenes, the microbial agent is used for treating lead and cadmium polluted soil, and the microbial agent comprises enterobacter aerogenes and klebsiella aerogenes; the enterobacter aerogenes is Enterobacter aerogenes W6, the Latin classification name of the enterobacter aerogenes is Enterobacter aerogenes, the enterobacter aerogenes is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is July 13, 2021, and the preservation number is CGMCC No.22888. The enterobacter aerogenes can be used for preparing the feed additive, and the feed additive can be used for preparing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive for producing the feed additive. The Klebsiella aerogenes are Klebsiella aerogenes Wn, the classification name of the Klebsiella aerogenes is Klebsiella aerogenes Wn, the Klebsiella aerogenes Wn is the Klebsiella aerogenes Wn, the Klebsiella aerogenes Wn is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is July 13, 2021, and the preservation number is CGMCC No. 22889.

Description

technical field [0001] The invention relates to microorganisms and applications thereof, in particular to a bacterial agent, composition and application of Enterobacter aerogenes combined with Klebsiella aerogenes. Background technique [0002] After decades of industrial development, a large amount of mining and smelting has caused the heavy metal content in the soil to exceed the standard, posing a threat to human survival. According to statistics, the average annual discharge of lead (Pb) in the world is about 5 million tons and cadmium (Cd) is about 30,000 tons. Lead and cadmium pollution in soil has the ability to migrate. When subjected to rainwater leaching, vertical infiltration, surface runoff, etc., the lead and cadmium content in surface water and groundwater will exceed the standard. Drinking groundwater polluted by heavy metal lead and cadmium or eating food chain products polluted by lead and cadmium may cause cancer-causing, disease-causing and mutagenic heal...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/22C12R1/01
CPCC12N1/20B09C1/105Y02P10/20
Inventor 杨志辉刘梓欣廖骐杨卫春赵飞平禹林李鹏刚司梦莹
Owner CENT SOUTH UNIV
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