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Sample for verifying capacity of total coliform flora in domestic drinking water and preparation method thereof

A technology of drinking water and coliform bacteria, applied in the direction of biochemical equipment and methods, measurement/testing of microorganisms, resistance to vector-borne diseases, etc., can solve problems such as food-borne diseases

Inactive Publication Date: 2017-09-22
中检科(北京)测试认证有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the total coliform count exceeds a certain limit, it increases the risk of pathogenic E. coli and eventually leads to foodborne illness

Method used

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  • Sample for verifying capacity of total coliform flora in domestic drinking water and preparation method thereof
  • Sample for verifying capacity of total coliform flora in domestic drinking water and preparation method thereof
  • Sample for verifying capacity of total coliform flora in domestic drinking water and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Total coliform proficiency test samples in drinking water, including target flora and background flora, the target flora consists of Escherichia coli (E.coil), Klebsiella pneumoniae (Klebsiella pneumoniae), cloacae The background flora consisted of Staphylococcus aureus and Bacillus cereus.

[0030] The sample is based on trehalose, skimmed milk powder and sterile water, wherein the volume fraction of trehalose is 12%, and the volume fraction of skimmed milk powder is 0.1%.

[0031] The target concentration of the target flora in the sample is 10 2 ~10 3 CFU / 100mL, the target concentration of background flora is 10 2 CFU / mL.

Embodiment 2

[0033] Such as figure 1 Shown, a kind of preparation method of the total coliform group proficiency verification sample in drinking water in embodiment 1, comprises the selection of sample addition bacterial strain, the preparation of lyoprotectant, sample lyophilization, sample homogeneity and stability test Four steps, the specific steps are as follows:

[0034] 1. Selection of sample addition strains

[0035] By target flora: Escherichia coli (E.coil), Klebsiella pneumoniae (Klebsiella pneumoniae), Enterobacter cloacae (Enterobacter cloacae), Proteus mirabilis (Proteus mirabilis) and Enterobacter aerogenes (Enterobacter aerogenes) , Background flora: Staphylococcus aureus (Staphylococcus aureus), Bacillus cereus (Bacillus cereus) selected standard strains, all standard strains were purchased from institutions designated by the government, and a strain certificate was attached to ensure the traceability of the strains.

[0036] 2. Preparation of lyoprotectant

[0037] The...

Embodiment 3

[0070] The steps of the preparation method of the total coliform proficiency test sample in drinking water described in this example are the same as in Example 2, and the different technical parameters are: in the preparation of the bacterial suspension, in the target bacterial suspension The concentration of each target bacteria was 5×10 3 CFU / 100mL preparation, the concentration of each background bacteria in the background flora suspension according to 10 2 CFU / mL preparation; freeze-drying process 45h.

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Abstract

The invention belongs to the field of quality control of microbiological detection, and particularly relates to a sample for verifying capacity of total coliform flora in domestic drinking water and a preparation method thereof. The sample for verifying the capacity of the total coliform flora in the domestic drinking water comprises target flora and background flora, wherein the target flora consists of escherichia coli, klebsiella pneumoniae, enterobacter cloacae, proteus mirabilis and enterobacter aerogenes; the background flora consists of staphylococcus aureus and bacillus cereus. The uniformity and the stability of the sample meet capacity verifying requirements, so that the stability of a test sample can be ensured to the maximum extent, and even during transportation and storage, change of the escherichia coli content does not affect the total amount of the total coliform flora in the statistical sense; the preparation method of the sample is simple in process and high in successful rate.

Description

technical field [0001] The invention belongs to the quality control field of microbiological detection, and in particular relates to a sample for proficiency verification of total coliform bacteria in drinking water and a preparation method thereof. Background technique [0002] The field of microbiological testing of drinking water is very special. At present, although there are domestic and foreign precedents for accreditation institutions to organize drinking water microbiological proficiency testing and large-scale preparation of microbial proficiency testing samples, most of them are qualitative samples of total coliforms or pure strains, but samples for quantitative testing of total coliforms The preparation is difficult, and there is no better preparation method, and the uniformity and stability cannot meet the requirements of the total coliforms, that is, the total coliforms standard sample is still a blank field. [0003] The total coliform group is not a bacteriol...

Claims

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Application Information

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IPC IPC(8): C12Q1/10C12Q1/06C12Q1/24
CPCC12Q1/06C12Q1/10C12Q1/24Y02A50/30
Inventor 卢行安徐大军李宏赵红阳王秀君刘汉霞王伟陈若晞
Owner 中检科(北京)测试认证有限公司
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