Sika deer coronet antibacterial peptides/proteins with antibacterial effect and applications thereof
A technology of sika antlers and antimicrobial peptides, which is applied in the field of biological proteins, can solve the problem of not being able to comprehensively and objectively reflect the antibacterial conditions of sika antler proteins and peptides
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[0069] An antibacterial peptide / protein of sika antler disc with antibacterial effect, including sika antler disc small molecular polypeptide, sika antler disc collagen hydrolyzed peptide and sika antler disc small molecule protein monomer.
[0070] The small molecule polypeptide of sika antler disc is extracted according to the following steps:
[0071] A. Extraction of total peptides from antler discs:
[0072] Weigh 500g of antler disc powder, add 3000mL of acetic acid-sodium acetate buffer solution with a pH of 3.5 fully pre-cooled in a refrigerator at 4°C, stir thoroughly with a magnetic stirrer, centrifuge the homogenate, and take the supernatant; slowly add 4 Fully pre-cool 95% ethanol in the refrigerator until the final concentration of the supernatant reaches 55%, place in the refrigerator at 4°C and stir once every 1 hour, centrifuge after 4 hours, and take the supernatant; the above operations are performed at 4°C except for rotary evaporation under;
[0073] The ...
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