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Sika deer coronet antibacterial peptides/proteins with antibacterial effect and applications thereof

A technology of sika antlers and antimicrobial peptides, which is applied in the field of biological proteins, can solve the problem of not being able to comprehensively and objectively reflect the antibacterial conditions of sika antler proteins and peptides

Inactive Publication Date: 2017-05-31
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, the research on the activity of the water-soluble total peptides of the antler discs is mostly concentrated on animal experiments and anti-tumor cell research, and there are few reports on the antibacterial effect; The product is collagen hydrolysis peptide) most of the research on the anti-oxidation effect; the research on the antibacterial effect of sika antler disc only focuses on the protein and polypeptide monomer obtained by partial separation and purification, and because of these antibacterial experimental research Most of the bacteria used are no more than 4 kinds so that the actual bacteriostasis of the sika antler disc protein and polypeptide cannot be fully and objectively reflected. Therefore, the present invention has carried out preliminary research on the bacteriostatic activity of the sika antler disc protein polypeptide

Method used

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  • Sika deer coronet antibacterial peptides/proteins with antibacterial effect and applications thereof
  • Sika deer coronet antibacterial peptides/proteins with antibacterial effect and applications thereof
  • Sika deer coronet antibacterial peptides/proteins with antibacterial effect and applications thereof

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Embodiment 1

[0069] An antibacterial peptide / protein of sika antler disc with antibacterial effect, including sika antler disc small molecular polypeptide, sika antler disc collagen hydrolyzed peptide and sika antler disc small molecule protein monomer.

[0070] The small molecule polypeptide of sika antler disc is extracted according to the following steps:

[0071] A. Extraction of total peptides from antler discs:

[0072] Weigh 500g of antler disc powder, add 3000mL of acetic acid-sodium acetate buffer solution with a pH of 3.5 fully pre-cooled in a refrigerator at 4°C, stir thoroughly with a magnetic stirrer, centrifuge the homogenate, and take the supernatant; slowly add 4 Fully pre-cool 95% ethanol in the refrigerator until the final concentration of the supernatant reaches 55%, place in the refrigerator at 4°C and stir once every 1 hour, centrifuge after 4 hours, and take the supernatant; the above operations are performed at 4°C except for rotary evaporation under;

[0073] The ...

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Abstract

The invention provides sika deer coronet antibacterial peptides / proteins with antibacterial effect and applications thereof, including sika deer coronet micromolecular polypeptides, sika deer coronet collagen hydrolyzing peptides, and sika deer coronet micromolecular protein monomers. The peptides / proteins are subjected to inhibition ring tests so as to observe the inhibiting effect on Gram-negative bacteria, Gram-positive bacteria, and fungi; wherein the Gram-negative bacteria comprise enterobacter aerogenes, proteus mirabilis, pseudomonas aeruginosa, and escherichia coli. The Gram-positive bacteria comprise staphylococcus lentus, lactococcus garvieae, staphylococcus xylosus, bacillus subtilis, and staphylococcus intermedius. The fungi are candida albicans. Under the effect of various bacteriostatic agents, the enterobacter aerogenes, bacillus subtilis, escherichia coli, pseudomonas aeruginosa, staphylococcus intermedius, and staphylococcus xylosus are inhibited to a certain degree, and the candida albicans, lactococcus garvieae, proteus mirabilis, and staphylococcus lentus are not inhibited.

Description

technical field [0001] The invention relates to the technical field of biological proteins, in particular to a sika antler antibacterial peptide / protein with antibacterial effect and application thereof. Background technique [0002] Antler discs refer to the ossifications left on the horn handles of male deer after sawing the antlers. The disc-shaped substances that fall off automatically before the new antlers grow in the second year are also named "deer discs" and "antler caps". They mainly contain A variety of essential amino acids and a variety of mineral elements. The antler plate is helmet-shaped or flat-helmet-shaped, with a diameter of 2-5cm and a height of 2-4.5cm. The surface is grayish-yellow or taupe, honeycomb-shaped, the bottom surface is relatively flat and has a certain luster, and the upper part presents an irregular hemispherical shape. , the texture is relatively hard, and the color is off-white. In recent years, the composition of antler discs has been...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C07K1/20C07K1/34C12P21/06A61P31/04
CPCC12P21/06C07K1/145C07K1/20C07K1/34Y02A50/30
Inventor 胡薇
Owner JILIN AGRICULTURAL UNIV
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