The invention discloses
antimicrobial peptide HP10, a preparing method thereof and an application thereof, and belongs to the technical field of synthesis of
peptide with 5-20 pieces of
amino acid and the technical field of specific curative activity of compounds and medicinal preparations. The
amino acid sequence of the
antimicrobial peptide HP10 is HHHLHHIHKP, the molecular weight of the
antimicrobial peptide HP10 is 1,292.4 Da, and the
isoelectric point of the antimicrobial peptide HP10 is 8.79. The preparing method of the antimicrobial peptide HP10 includes the following steps that 1, the antimicrobial peptide HP10 with the
amino acid sequence of HHHLHHIHKP is activated and crosslinked from the C end to the N end with an FMOC protection synthesis method; 2,
chromatography,
desalination and purification are carried out on the product of the step 1 through an HPLC reversed-phase column, and the antimicrobial peptide HP10 is obtained. The antimicrobial peptide HP10 has the advantages that the molecular weight is small, artificial synthesis is convenient,
heat stability is high, the sterilizing effect is high, and
drug resistance is avoided; the antimicrobial peptide HP10 further has the advantages that when the antimicrobial peptide HP10 is applied to piglet feed, the intestinal health of piglets can be remarkably improved, body
immunity can be improved, and the growth performance of the piglets can be improved.