66 results about "Cathelicidin antimicrobial peptide" patented technology

The invention provides methods for the treatment of disease and promotion of healing that include providing a therapeutically effective amount of a mammalian antimicrobial peptide (AMP) or analog thereof, in particular a cathelicidin or cathelicidin fragment or cathelicidin analog, thereby treating the disease in the subject in need thereof. The invention also provides specific analogs or fragments of cathelicidin that function as agonists, as do endogenous cathelicidins, or as either dominant negatives or as inhibitors to endogenous cathelicidin or to other endogenous AMPs or that compete with pro-inflammatory agents or fragments of AMPs on cognate receptors without inducing disease.

Use of the antimicrobial cathelicidin peptide ll-37, N-terminal fragments of LL-37 or extended sequences of LL-37 having 1-3 amino acids in the C-terminal end, for stimulating proliferation of epithalial and stromal cells and thereby healing of wounds, such as chronic ulcers. The cytotoxic effect of LL-37 may be reduced by including a bilayer-forming polar lipid, especially a digalactosyldiacylglycerol, in pharmaceutical compositions and growth media comprising LL-37.

Antimicrobial peptides and methods for their use are provided. The peptides are optimized, truncated versions of chicken cathelicidins (“fowlicidins”).

The invention relates to antimicrobial cathelicidin polypeptides related to a 38 amino acid peptide having SEQ ID NO:4. The invention provides for polypeptides having broad spectrum antimicrobial activity, nucleic acids and expression vectors encoding such polypeptides, as well as host cells and methods of reducing survival of a microbe. In addition, the invention also provides compositions, as well as articles of manufacture, that comprise a broad spectrum antimicrobial polypeptide.

The invention relates to a variant cathelicidin-BF15 of bungarus fasciatus antibiosis peptide cathelicidin-BF and application thereof. The invention belongs to the field of biomedicine. The variant cathelicidin-BF15 of the bungarus fasciatus antibiosis peptide cathelicidin-BF is a straight chain polypeptide which contains 15 amino acid residues with the molecular weight of 1,939.47Da and an isoelectric point of 12.05. The total sequence of the cathelicidin-BF15 is as follows:valine-lysine-arginine-phenylalanine-lysine-lysine-phenylalanine-phenylalanine-arginine-lysine-leucine-lysine-lysine-serine-valine. The cathelicidin-BF15 which has strong bactericidal action can exterminate a plurality of clinical drug-fast bacteria. In addition, the cathelicidin-BF15 is also characterized by simple structure, convenient synthesis, broad spectrum antibiosis, and the like.

The invention discloses a compound premix for preventing and treating dairy cow mastitis recessive, and a preparation method and application thereof. The compound premix comprises the following components in percentage by mass: 15 to 25 percent of dandelion extract, 15 to 25 percent of honeysuckle extract, 5 to 15 percent of ricepaper pith extract, 5 to 15 percent of motherwort herb extract, 25 to 35 percent of biochemical fulvic acid, and 5 to 15 percent of antimicrobial peptide. According to the compound premix prepared by the preparation method, the occurrence rate of dairy cow mastitis recessive can be efficiently and stably reduced, the constitution of dairy cow can be reduced, and drugs are not remained. The compound premix can be applied to industrial production and practical operation in large scale.

The invention relates to the field of biomedicine and in particular relates to an antibacterial polypeptide. Part of active amino acids of the antibacterial polypeptide of Cathelicidin family is mutated based on the antibacterial polypeptide of Cathelicidin family, and then the antibacterial polypeptide with high inhibitory activity to penicilin-resistant bacterium is obtained by solid phase chemical synthesis. The result of pharmacodynamic tests shows that the antibacterial polypeptide provided by the invention has good in vitro antibacterial activity and is applicable to treating diseases about drug-resistant bacterial infection.

The invention relates to a preparation method of a submicron emulsion repairing stock solution composition and a cosmetic composition containing the submicron emulsion repairing stock solution composition. The submicron emulsion repairing stock solution composition is prepared from oligopeptide-3, oligopeptide-1, Indolicidin or an analogue thereof, Cathelicidin-BF, hyaluronic acid, glycerol, minkoil, lecithin, starch sodium octenylsuccinate, fatty alcohol polyoxyethylene ether, hydroxyethyl cellulose, propylene glycol / bis(hydroxymethyl)imidazolidinyl urea / methylparaben / iodopropynyl butylcarbamate and the like. A submicron emulsion repairing stock solution has the effects of keeping moisture and repairing, resisting bacteria and removing acnes. The repairing stock solution composition canbe used for promoting growth and cracking of various cells in epidermal tissues, promoting the growth of collagen, repairing aged collagen elastic fibers and recovering skin activity; especially, thesubmicron emulsion repairing stock solution composition has remarkable repairing effects on various epidermal wounds including acne marks, phototherapy, scalds and the like.

The invention discloses a rat-pig intestinal epithelial cell integrated model established by lipopolysaccharide (LPS) stimulation. Bacterial lipopolysaccharide (LPS) has a function in damaging intestinal epithelial barrier, and the integrated model disclosed by the invention aims at identifying whether an antimicrobial peptide Cathelicidin-BF (C-BF) derived from a snake has a protective effect on LPS-induced enteritidis or not. IPEC-J2 is an intestinal epithelial cell line isolated from the porcine jejunum segment and the porcine digestive system is very similar to the human digestive system. By in vivo rat model and in vitro porcine IPEC-J2 cell model, the protective effect of Cathelicidin-BF on impaired LPS-induced intestinal barrier function is tested. The test results show that the Cathelicidin-BF has protective effects on impaired LPS-induced intestinal barrier function and LPS-induced IPEC-J2 cell damage model.

The invention discloses skin repair peptide cathelicidin-NV of nanorana pleskei as well as a gene and application thereof, and belongs to the field of biomedicines. The skin repair peptide cathelicidin-NV is a ring type polypeptide encoded by a defense peptide gene of nanorana ventripunctata which is a special species belongs to the Chinese amphibian; the molecular weight is 2845.9 Dalton, and theisoelectric point is 9.90; the amino acid sequence of the skin repair peptide cathelicidin-NV is shown in SEQ ID NO: 1. The gene GenBank accession No.(MH010580) for encoding a skin repair peptide cathelicidin-NV precursor comprises 652 nucleotide sequences, and each nucleotide sequence is shown in SEQ ID NO: 2; nucleotides at sites from 367 to 438 are encoding genes of the skin repair peptide cathelicidin-NV of the nanorana pleskei. The invention further discloses the application of the skin repair peptide cathelicidin-NV of the nanorana pleskei in preparation of a treatment drug for promoting skin wound repairing. The skin repair peptide cathelicidin-NV has the beneficial effect that the skin repair peptide cathelicidin-NV has a relatively strong effect of promoting wound healing of a skin wound, and the wound healing promoting effect of the skin repair peptide cathelicidin-NV is similar to that of an epidermal growth factor (EGF).

The invention relates to defensin cathelicidin-PP of polypedates puerensis as well as a gene and application thereof, and belongs to the field of biomedicine. The defensin cathelicidin-PP is a cyclic polypeptide encoded by a gene of defensin of Chinese amphibian polypedates puerensis, and has a molecular weight of 3,366.08 daltons and an isoelectric point of 10.05; the amino acid sequence of the defensin cathelicidin-PP is as shown by SEQ ID NO: 1. A gene GenBank Accession KY610282 for encoding a precursor of the defensin cathelicidin-PP of the polypedates puerensis consists of 615 nucleotide sequences; the nucleotide sequence of the gene GenBank Accession KY610282 is as shown by SEQ ID NO: 2, wherein nucleotides at No. 346 to No. 441 sites are encoding genes of defensin cathelicidin-PP of mature polypedates puerensis. The invention relates to the application of the defensin cathelicidin-PP of the polypedates puerensis to the preparation of a therapeutic medicine for infectious diseases caused by escherichia coli, salmonella paratyphi A, pseudomonas aeruginosa and candida glabrata. The defensin cathelicidin-PP has the obvious effects of inhibiting the growth of a bacterium and a fungus for the escherichia coli, the salmonella paratyphi A, the pseudomonas aeruginosa and the candida glabrata.

The invention discloses children antimicrobial peptides cathelicidins, a preparation method and the applications thereof. The nucleotide sequence and the amino acid sequence of children antimicrobial peptides Cathelicidin1, Cathelicidin2 and Cathelicidin3 are shown as a sequence table. The preparation method of the antimicrobial peptides adopts a solid-phase chemical synthesis method or a geneticengineering method. The genetic engineering method comprises the following steps of: respectively cloning the encoding genes of mature children antimicrobial peptides Cathelicidin1-3 to expression vectors; and then, guiding into host cells to express. By applying the genetic engineering technology in the invention, the children antimicrobial peptides Cathelicidins are expressed in the prokaryotichost cells with high efficiency. Proved by experiments, the antimicrobial peptides have obvious inhibition on various gram-positive bacteria and negative bacteria.

Endogenously produced cationic antimicrobial peptides are ubiquitous components of host defenses in mammals, birds, amphibia, insects, and plants. Cationic peptides are also effective when administered as therapeutic agents. A practical drawback in cationic peptide therapy, however, is the cost of producing the agents. The methods described herein provide a means to efficiently produce cationic peptides from recombinant host cells. These recombinantly-produced cationic peptides can be rapidly purified from host cell proteins using anion exchange chromatography.

The invention discloses antimicrobial peptide HP10, a preparing method thereof and an application thereof, and belongs to the technical field of synthesis of peptide with 5-20 pieces of amino acid and the technical field of specific curative activity of compounds and medicinal preparations. The amino acid sequence of the antimicrobial peptide HP10 is HHHLHHIHKP, the molecular weight of the antimicrobial peptide HP10 is 1,292.4 Da, and the isoelectric point of the antimicrobial peptide HP10 is 8.79. The preparing method of the antimicrobial peptide HP10 includes the following steps that 1, the antimicrobial peptide HP10 with the amino acid sequence of HHHLHHIHKP is activated and crosslinked from the C end to the N end with an FMOC protection synthesis method; 2, chromatography, desalination and purification are carried out on the product of the step 1 through an HPLC reversed-phase column, and the antimicrobial peptide HP10 is obtained. The antimicrobial peptide HP10 has the advantages that the molecular weight is small, artificial synthesis is convenient, heat stability is high, the sterilizing effect is high, and drug resistance is avoided; the antimicrobial peptide HP10 further has the advantages that when the antimicrobial peptide HP10 is applied to piglet feed, the intestinal health of piglets can be remarkably improved, body immunity can be improved, and the growth performance of the piglets can be improved.

Agents and natural and synthetic formulations and extracts are useful in control of pathogen infection and infestation in humans, animals and plants, as well as pathogen contamination in environmental locales. A method inhibits growth or infection or infestation of a pathogen, including contacting the pathogen with an effective amount of a combination of a plant defensin or a functional natural or synthetic derivative or variant thereof and a non-defensin peptide, such as a cathelicidin peptide, a hairpin peptide, a hairpin peptide from which disulfide bridges have been removed, indolicidin, synthetic peptide CP-29, or a peptide derived from a cystatin. The combination of the defensin and peptide are synergistic compared to the use of each alone at the same individual dose as used in the combination.

Provided are methods for the treatment of disease and promotion of healing that include providing a therapeutically effective amount of a mammalian antimicrobial peptide (AMP) or analog thereof, in particular a cathelicidin or cathelicidin fragment or cathelicidin analog, thereby treating the disease in the subject in need thereof. Also provided are specific analogs or fragments of cathelicidin that function as agonists, as do endogenous cathelicidins, or as either dominant negatives or as inhibitors to endogenous cathelicidin or to other endogenous AMPs or that compete with pro-inflammatory agents or fragments of AMPs on cognate receptors without inducing disease.

The invention discloses [delta]Tlrgt2 trichoderma engineering bacteria with high yield of peptaibols and a construction method and application thereof. A Tlrgt2 knockout vector is constructed by upstream and downstream homologous arm sequences of a Tlrgt2 gene and a hygromycin resistance gene hph sequence, the [delta]Tlrgt2 trichoderma engineering bacteria is prepared by a gene knockout method, aTlrgt2 gene is knocked out, and the peptaibols production capacity of the bacteria can be effectively improved. The stable passage of the constructed [delta]Tlrgt2 trichoderma engineering bacteria canbe achieved, the yield of the trichoderma peptaibols can be significantly increased, and the production of the peptaibols by the [delta]Tlrgt2 trichoderma engineering bacteria is about 12 times higher than that of an original strain. The application of the trichoderma peptaibols in medicine and agriculture is facilitated.

The invention discloses a pinctada martensii antimicrobial peptide PmAMP and application thereof.The amino sequence of the inctada martensii antimicrobial peptide PmAMP is as shown in SEQ ID No.1.The pinctada martensii antimicrobial peptide PmAMP is characterized in that pinctada martensii is used as the research object, the serum of the pinctada martensii is separated and purified to screen out protein with antimicrobial activity, the obtained pinctada martensii antimicrobial peptide PmAMP can evidently inhibit the growth of escherichia coli and micrococcus luteus, the gene for encoding the antimicrobial peptide PmAMP are obtained through mass spectrum sequencing and using the RACE technology to clone the overall length of cDNA, and the nucleotide sequence of the gene is as shown in SEQ ID No.2 or SEQ ID No.3.When fluorescent quantitative PCR is used to detect the expression content of the PmAMP in tissue, the detection results show that the expression content of the PmAMP is the highest in adductor muscle and low in blood cells.After bacterium stimulation, the expression content of the PmAMP in the blood cells is the highest after 8 hours, so that the PmAMP is immune protein.By the pinctada martensii antimicrobial peptide PmAMP, a foundation is laid for the immunology researches and disease control of the pinctada martensii.

The invention discloses canine chewing gum with an oral care coating. The canine chewing gum comprises an inner core and the oral care coating for externally covering the surface of the inner core. The inner core is prepared by amylose, an edible binder, grease, insoluble cellulose, soluble dietary cellulose, a humectant, and a flavor agent. In solution of the oral care coating, a mass percentageof an effective component is 0.1-5%, and the rest is water. The effective component is a functional component and an auxiliary component. The functional component is formed by a persimmon extract, a grapefruit seed extract, a honeysuckle extract, a cranberry extract, a brown seaweed extract, a green tea extract, a yucca extract, tricortin, an antibacterial peptide, lysozyme, a negative ion salt vitamin, propolis extracting solution, calcium gluconate, polyphosphate, water-soluble silicon, and tea polyphenol; and the auxiliary component is arbutin. The canine chewing gum is capable of, in allusion to defects of an existing product, covering the surface of the inner core without water absorption or with slow water absorption by the oral care coating, and developing a multi-layer care effect.

The invention provides a pseudosciaena crocea hepcidin gene yeast expression product as well as a preparation method and application thereof, and relates to a pseudosciaena crocea hepcidin gene. The preparation method comprises the following steps of: cloning a full-length cDNA (Complementary Deoxyribonucleic Acid) sequence of the gene, amplifying a gene segment, then cloning into a pichia pastoris expression vector, and constructing to obtain a recombinant expression vector; converting a linearized recombinant expression vector into a pichia pastorris SMD1168 strain by electric shock, screening, carrying out PCR (Polymerase Chain Reaction) amplification and identification on a genome to obtain pichia pastorris SMD 1168 / pPICZalpah A-hepcidin, converting the expression vector by electric shock into pichia pastorris SMD 1168 strain serving as a contrast strain, inducing and culturing in a culture medium, centrifuging to collect supernatant, and obtaining a secreting type expression in the supernatant; purifying a recombination protein; adding NaCl to the supernatant till the final concentration is 0.2M, filtering liquid supernatant passing through columns to be subjected to column chromatography isolation, eluting a target protein, and dialyzing the purified protein, thus obtaining the pseudosciaena crocea hepcidin gene yeast expression product.

The invention discloses a high-moisture facial mask based on cellulose fibers and a preparation method of the high-moisture facial mask. The moisturizing facial mask comprises a modified facial mask base cloth and a facial mask base solution, and the facial mask base solution comprises the following raw materials in parts by weight: 8 to 10 parts of sodium hyaluronate, 8 to 12 parts of carbomer, 6to 8 parts of antibacterial peptide, 3 to 4 parts of N,N-dicyclohexylcarbodiimide, 10 to 15 parts of triethanolamine, 8 to 10 parts of glycerinum, 3 to 5 parts of collagen, 4 to 7 parts of sodium methylparaben and 6 to 8 parts of a traditional Chinese medicine additive, w herein the modified facial mask base cloth is prepared by treating a facial mask base cloth with isopropanol, sodium hydroxide, tetramethylammonium hydroxide, epoxypropane and glucose oxidase. The process design is reasonable, the component ratio is suitable, the prepared moisturizing facial mask has excellent effects of water supplementing, moisturizing and skin moisturizing, further has an excellent antibacterial effect, can inhibit the microbial growth on skin surfaces and avoid acne occurrence, and has better practicality.

The invention relates to a Brachymystax lenok Cathelicidin antimicrobial peptide CATH_BARLE, and a gene, a preparation and an application thereof, and belongs to the technical field of biomedicine. The gene of the CATH_BARLE is composed of 909 nucleotides, and a part coding the mature peptide is nucleotides from the 439th bit to the 597th bit. The mature peptide CATH_BARLE is rich in alkaline amino acids, and has a strong bactericidal effect on common pathogens in aquatic product culture. The CATH_BARLE has a simple structure, does not contain a disulfide bond or an annular structure, and is convenient for chemical synthesis and gene engineering preparation; and the CATH_BARLE also has a good killing effect on many clinical drug-resistant bacteria, especially fungi.

The invention belongs to the technical field of polypeptide drugs in biochemistry, and particularly relates to an application of tylototriton verrucosus skin repair peptide cathelicidin-TV2. The tylototriton verrucosus skin repair peptide cathelicidin-TV2 is obtained according to a conventional chemical synthesis method; the molecular weight of the tylototriton verrucosus skin repair peptide cathelicidin-TV2 is 1631.98Da, the isoelectric point is 11.02, and the primary structure of the complete sequence of the tylototriton verrucosus skin repair peptide cathelicidin-TV2 is RKCVRQNNKRVCK. The tylototriton verrucosus skin repair peptide cathelicidin-TV2 disclosed by the invention has the functions of reducing scar generated by skin trauma and accelerating the treatment or repair of scars, and can be applied to preparation of drugs for treating skin trauma, reducing scars and accelerating scar repair.

The invention discloses a comprehensive model for protecting mice and curing mice macrophage system UC (Ulcerative Colitis) by C-BF (Cathelicidin-BF). C-BF has broad-spectrum antibacterial activity, especially for gram-negative bacteria. IBDS (Inflammatory Bowel Diseases) include UC and CD (Crohn's Disease), and the pathological mechanisms of IBDS are not clear. The comprehensive model provided by the invention combines in-vivo mice experiments and in-vitro cell experiments to evaluate the effects of C-BF on relieving inflammations, reducing the cell apoptosis level and improving the intestinal barrier function.

The present invention provides new derivatives of CATH2 or CMAP27, one of the cathelicidins. These derivatives comprise N-terminally truncated peptides, cyclic peptides, D-amino acid variants of CATH2 and its truncated derivatives, inverso and retro-inverso CATH2 derivatives. These derivatives are useful as anti-infectives, in vaccines, and especially for in ovo applications. Further, for the above derivatives and also for the already known C-terminally truncated derivatives new immunoactivating functions have been described that are particularly advantageous for prophylactic treatments.

A food processing equipment disinfectant consists of the following raw materials by weight: 3-7 parts of fatty alcohol polyoxyethylene ether, 3-7 parts of sodium carbonate, 4-6 parts of citric acid, 1.2 -2.4 parts of Neroli oil, 6-9 parts of Hibiscus mutabilis L., 9-14 parts of japanese stephania root, 1-4 parts of glutaraldehyde, 4-7 parts of iodoanisole, 3-6 parts of zinc acetate, 1.2-2 parts of Tween, 2-4 parts of antimicrobial peptides, 1.5-3 parts of a thickener 2-3 parts of potassium iodide and 13-16 parts of distilled water. The invention has the beneficial effects that the food processing equipment disinfectant has excellent sterilization and disinfection effects, no harm to equipment and operating workers and good durability.

A method for diagnosing tuberculous meningitis (TBM) is described herein. A fluid sample from a subject suspected of having TBM is tested for the presence of at least two of Interleukin-13 (IL-13), Vascular endothelial growth factor (VEGF), Cathelicidin (LL-37), IL- 7, IL-12p70, IFN-gamma, IL-6, IL-10, IL-13, IP-10, MIP-1a, MIP-1b, RANTES and GM-CSF. Increased levels of at least any two of these biomarkers in the sample compared to levels in subjects without TBM are indicative that the subject has TBM.

A polytherapy of orally available compounds is disclosed that synergistically modulates and induces the expression of the cathelicidin gene (CAMP), which encodes the host defense peptide LL-37. By providing a number of different CAMP-inducing compounds together at the same time, stronger gene induction is achieved than with just one or two compounds, because the mechanism of induction broadens. Induction also may vary in different parts of the body depending on which compounds are used, and at what levels. We show for the first time that the polytherapy can induce cathelicidin expression in the brain, which may help to treat or prevent Alzheimer's Disease. Systemic cathelicidin gene induction may help treat numerous other conditions including Type 2 Diabetes / Metabolic Syndrome, or chronic bacterial, viral, or fungal infections associated with increased cancer risk or neurodegeneration. By increasing cellular autophagy and macroautophagy and supporting mitochondrial biogenesis and homeostasis, CAMP gene upregulation may reduce the effects of cellular aging and increase longevity.