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Rat-pig intestinal epithelial cell integrated model established by lipopolysaccharide (LPS) stimulation

An epithelial cell, comprehensive model technology, used in the field of biomedicine

Inactive Publication Date: 2014-09-10
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, whether the antimicrobial peptide C-BF has a protective effect on the maintenance and reconstruction of intestinal barrier function has never been studied

Method used

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  • Rat-pig intestinal epithelial cell integrated model established by lipopolysaccharide (LPS) stimulation
  • Rat-pig intestinal epithelial cell integrated model established by lipopolysaccharide (LPS) stimulation
  • Rat-pig intestinal epithelial cell integrated model established by lipopolysaccharide (LPS) stimulation

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Embodiment Construction

[0027] The present invention will be described in detail below in conjunction with the accompanying drawings, and the embodiments are only for describing but not limiting the present invention.

[0028] 1. C-BF can reduce the morphological changes of rat jejunal mucosa induced by LPS

[0029] To assess the potential role of C-BF in LPS-associated enteritis, we injected healthy adult rats (10 per group) intraperitoneally with LPS before and after the action of C-BF. As shown in Figure 1, LPS-treated rats developed enteritis accompanied by morphological changes in the intestinal mucosa, while adding C-BF before and after LPS injection could significantly reduce LPS-induced jejunal mucosal damage, which can be seen from H&E staining ( Figure 1A ). Significant reduction in villi height and increase in crypt depth were observed in the LPS-treated group (Table 1). Addition of 10 or 20 mg / kg C-BF before and after LPS treatment significantly increased villi height and decreased cryp...

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Abstract

The invention discloses a rat-pig intestinal epithelial cell integrated model established by lipopolysaccharide (LPS) stimulation. Bacterial lipopolysaccharide (LPS) has a function in damaging intestinal epithelial barrier, and the integrated model disclosed by the invention aims at identifying whether an antimicrobial peptide Cathelicidin-BF (C-BF) derived from a snake has a protective effect on LPS-induced enteritidis or not. IPEC-J2 is an intestinal epithelial cell line isolated from the porcine jejunum segment and the porcine digestive system is very similar to the human digestive system. By in vivo rat model and in vitro porcine IPEC-J2 cell model, the protective effect of Cathelicidin-BF on impaired LPS-induced intestinal barrier function is tested. The test results show that the Cathelicidin-BF has protective effects on impaired LPS-induced intestinal barrier function and LPS-induced IPEC-J2 cell damage model.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to the protective effect of Cathelicidin-BF on rat intestinal epithelial barrier dysfunction induced by lipopolysaccharide and the damage model of pig intestinal epithelial cell barrier induced by lipopolysaccharide. Background technique [0002] The small intestinal mucosal epithelium is a continuous single layer in vivo, and it plays a key role in the resistance to microbial-induced gastrointestinal diseases. Defects in barrier integrity, a major pathophysiological feature of mucosal inflammatory diseases, lead to increased permeability leading to leakage of water and plasma proteins into the lumen, with concomitant translocation of intestinal bacteria and / or microbial by-products into the systemic circulation leading to systemic sepsis . Therefore, maintaining the integrity of the intestinal epithelial barrier is important for intestinal homeostasis. It is well known that ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071C12Q1/68C12Q1/26C12Q1/02G01N33/53G01N21/64
Inventor 汪以真张海文金灵红
Owner ZHEJIANG UNIV
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