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Skin repair peptide cathelicidin-NV of nanorana pleskei as well as gene and application thereof

A technology of skin repair and bonobo, which is applied in the field of biomedicine to achieve a strong skin repair activity and a significant effect of promoting skin wound repair

Active Publication Date: 2018-09-04
KUNMING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Nanorana ventripunctata (Nanorana ventripunctata) is an amphibian of the genus Nanorana (Ranaidae) of the amphibian order Anura. It is a unique species in China and a unique species on the Qinghai-Tibet Plateau. The skin repair peptides of the cathelicidin family in its skin secretions have not been reported yet.

Method used

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  • Skin repair peptide cathelicidin-NV of nanorana pleskei as well as gene and application thereof
  • Skin repair peptide cathelicidin-NV of nanorana pleskei as well as gene and application thereof
  • Skin repair peptide cathelicidin-NV of nanorana pleskei as well as gene and application thereof

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Embodiment Construction

[0016] The present invention will be described in detail below in conjunction with the accompanying drawings.

[0017] 1. Preparation and amino acid sequence determination of cathelicidin-NV, a skin repair peptide from Pyrenophagus punctatus

[0018] 1. Collect the skin secretions of the frog

[0019] The live bonobo frog was cleaned with water, and placed in a cylindrical sample bottle with a diameter of 10 cm and a height of 10 cm. After ether stimulation for 2 minutes, the frog's back foam was rinsed with 10 ml of 0.1M pH 6.0 phosphate buffered saline (PBS). The secreted liquid was centrifuged at 12000 rpm for 15 minutes at 4°C, and the supernatant was stored at -80°C.

[0020] 2. Separation and purification of cathelicidin-NV, a skin repair peptide from Pyrenophagus chinensis

[0021] Using the supernatant collected above as a raw material, purify the skin repair peptide cathelicidin-NV of Pygnus chinensis according to the following procedures, and each component of the...

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Abstract

The invention discloses skin repair peptide cathelicidin-NV of nanorana pleskei as well as a gene and application thereof, and belongs to the field of biomedicines. The skin repair peptide cathelicidin-NV is a ring type polypeptide encoded by a defense peptide gene of nanorana ventripunctata which is a special species belongs to the Chinese amphibian; the molecular weight is 2845.9 Dalton, and theisoelectric point is 9.90; the amino acid sequence of the skin repair peptide cathelicidin-NV is shown in SEQ ID NO: 1. The gene GenBank accession No.(MH010580) for encoding a skin repair peptide cathelicidin-NV precursor comprises 652 nucleotide sequences, and each nucleotide sequence is shown in SEQ ID NO: 2; nucleotides at sites from 367 to 438 are encoding genes of the skin repair peptide cathelicidin-NV of the nanorana pleskei. The invention further discloses the application of the skin repair peptide cathelicidin-NV of the nanorana pleskei in preparation of a treatment drug for promoting skin wound repairing. The skin repair peptide cathelicidin-NV has the beneficial effect that the skin repair peptide cathelicidin-NV has a relatively strong effect of promoting wound healing of a skin wound, and the wound healing promoting effect of the skin repair peptide cathelicidin-NV is similar to that of an epidermal growth factor (EGF).

Description

technical field [0001] The invention relates to a skin repair peptide cathelicidin-NV of Nanorana ventripunctata and its gene and application, belonging to the field of biomedicine. Background technique [0002] The skin is the first barrier for the body to contact with the outside world, the first line of defense for the body to avoid dehydration, injury, and infection, and the barrier to maintain the stability of the internal environment and prevent the invasion of microorganisms and chemical substances. Large-area skin defects and refractory skin ulcers caused by various acute and chronic injuries such as inflammation, ulcers, severe burns, trauma, tumor surgery, and congenital deformities will trigger many local and systemic reactions. Bacteria can easily invade and multiply after skin defects, which can cause water and electrolyte disorders in the body, septic shock, hypermetabolic response, and multiple organ failure, making the body unable to maintain a normal self-st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/46C12N15/12A61K38/17A61P17/02
CPCA61K38/00A61P17/02C07K14/463
Inventor 木丽仙杨海龙武静刘彤米鍇沈艳杨媛吕靖
Owner KUNMING MEDICAL UNIVERSITY
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