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New cath2 derivatives

A derivative, CMAP27-technology, applied to CATH2. , the peptide domain of CMAP27

Active Publication Date: 2017-03-08
乌得勒支大学控股有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these have worked well, there is still a need for other active derivatives of CMAP27

Method used

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Examples

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preparation example Construction

[0042] Some broader summaries of methods that can be applied in the preparation of peptides are described in: W.F. Anderson, Nature 392 Supp., April 30, 1998, p. 25-30; Pharmaceutical Biotechnology, Ed.D.J.A. Crommelin and R.D. Sindelar , Harwood Academic Press, 1997, p.53-70, 167-180, 123-152, 8-20; Protein Synthesis: Methods and Protocols, Ed.R. Martin, Humana Press, 1998, p.1-442; Solid-Phase Peptide Synthesis, Ed.G.B. Fields, Academic Press, 1997, p.1-780; Amino Acid and Peptide Synthesis, Oxford University Press, 1997, p.1-89.

[0043] Novel peptides as disclosed herein can be readily prepared by those skilled in the art.

[0044] The CMAP27- or CATH2-derivatives of the present invention can be used alone or in combination in the form of a multimer. Suitable combinations of the peptides of the invention include concatemers of the peptides of the invention sequentially linked to each other by spacers, e.g., in the form of peptide dimers, peptide trimers, etc., wherein the...

Embodiment 1

[0077] Example 1 - Efficacy of CMAP-27 derivative peptides administered in ovo

[0078] DCATH2 peptide (complete D-amino acid analog of CMAP1-26) was suspended in cholesterol / PBS formulation. At the 18th day of the embryonic period, 100 μl of DCATH2-containing suspension was used to inject Rose 308 eggs in the amniotic fluid, and then returned to the incubator for incubation (at the embryonic day of about 21 days). Two control groups were injected in the amniotic fluid with the cholesterol / PBS formulation. Peptide doses of 1 mg / kg body weight were calculated based on an embryo weight of 22 g. After hatching, the birds were transferred to 4 pens per treatment (n=40 / group). 3 days after hatching, in addition to the negative control, 5 × 10 6 Dose of CFU / bird, all birds were inoculated subcutaneously with the avian pathogenic Salmonella enteritidis pt13a strain. Two days after the challenge, 12 birds per group were sacrificed and samples were collected for blood and organ ana...

Embodiment 2

[0083] Example 2 - Prevention of E. coli infection following in ovo administration of peptides.

[0084] DCATH2 peptide was suspended in cholesterol / PBS formulation. At the 18th day of the embryonic period, 100 μl of DCATH2-containing suspension was used to inject Rose 308 eggs in the amniotic fluid, and then returned to the incubator for incubation (at the embryonic day of about 21 days). Two control groups were injected in the amniotic fluid with the cholesterol / PBS formulation. Based on an embryo weight of 22 g, a DCATH2 peptide dose of 1 mg / kg body weight was calculated. After hatching, the birds were transferred to each treatment isolation unit (n=54 / group). 3 days after hatching, in addition to the negative control, 1 × 10 6 The dose of CFU / bird, all birds were inoculated with avian pathogenic Escherichia coli (Escherichia coli) 506 strain (UU strain) in the trachea. Two days after challenge, 13 birds per group were sacrificed and used to collect samples for blood an...

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Abstract

The present invention provides new derivatives of CATH2 or CMAP27, one of the cathelicidins. These derivatives comprise N-terminally truncated peptides, cyclic peptides, D-amino acid variants of CATH2 and its truncated derivatives, inverso and retro-inverso CATH2 derivatives. These derivatives are useful as anti-infectives, in vaccines, and especially for in ovo applications. Further, for the above derivatives and also for the already known C-terminally truncated derivatives new immunoactivating functions have been described that are particularly advantageous for prophylactic treatments.

Description

technical field [0001] The present invention relates to the field of antibiotics and immune stimulating substances and in particular to peptides having such activity, more particularly peptides derived from CMAP27, also known as CATH2. Background technique [0002] CATH2 or CMAP27 are members of the class of antimicrobial peptides. So far, various types of antimicrobial peptides have been isolated and sequenced from multiple sources over the past few decades (for a selected review, see: Otvos Jr., L. Cell. Mol. Life Sci. 2002, 59:1138; Otvos, Jr., L.J. Peptide Sci. 2000, 6:497; Tan, Y.-T. et al., Mol. Med. Today 2000, 6:309; Scott, M.G. and Hancock, R.E.W., Crit. Rev.Immunol.2000,20:407; Hancock, R.E.W. and Chapple, D.S.Antimicrob.AgentsChemother.1999,43:1317; Hetru, C. et al., In: Molecular Mechanisms of ImmuneResponses in Insects; Brey, P. and Hultmark, D. Editors, Chapman and Hall, London, 1998, pp. 40-66; Hancock, R.E.W. et al., Adv. Microb. Physiol. 1995 37:135; Vaara...

Claims

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Application Information

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IPC IPC(8): C07K14/465A61K38/17A61P31/04
CPCC07K14/465A61K38/00A61P3/02A61P31/00A61P31/04A61P37/04A61P43/00A61K38/17C07K7/08
Inventor 亨德里克·彼得·哈赫斯曼阿尔贝特·范·戴克埃德温·约翰内斯·阿德里亚努斯·费尔德赫伊曾
Owner 乌得勒支大学控股有限公司
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